한국가금학회지 (Korean Journal of Poultry Science)

  • 제43권3호
  • /
  • Pages.177-189
  • /
  • 2016
  • /
  • 1225-6625(pISSN)
  • /
  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지
DOI QR코드

DOI QR Code

닭의 스트레스 연관 표지인자들의 발현도와 생산능력 간의 상관 분석

The Relationship of the Expressions of Stress-related Markers and Their Production Performances in Korean Domestic Chicken Breed

  • 박지애 (경남과학기술대학교 동물생명과학과) ;
  • 조은정 (경남과학기술대학교 동물생명과학과) ;
  • 최은식 (경남과학기술대학교 동물생명과학과) ;
  • 홍영호 (중앙대학교 생명자원공학부) ;
  • 최연호 (신구대학교 자원동물과) ;
  • 손시환 (경남과학기술대학교 동물생명과학과)
  • Park, Ji Ae (Dept. of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Cho, Eun Jung (Dept. of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Choi, Eun Sik (Dept. of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Hong, Yeong Ho (School of Bioresource and Bioscience, Chung-Ang University) ;
  • Choi, Yeon Ho (Dept. of Animal Science, Shingu College) ;
  • Sohn, Sea Hwan (Dept. of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology)
  • 투고 : 2016.08.26
  • 심사 : 2016.09.12
  • 발행 : 2016.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 닭의 개체 별 스트레스 연관 표지의 발현도와 이들의 생산능력 간의 연관성을 살펴보기 위한 것으로 토종종계 25계통을 대상으로 텔로미어 함량, 열 스트레스 단백질(HSPs) 유전자 발현율 및 DNA 손상율을 분석하고, 동일 개체들의 생존율, 성장능력 및 산란능력을 조사하여 이들 간의 상관관계를 분석 고찰하였다. 분석 결과, 생산능력 및 스트레스 표지 값들 간에 계통별로 유의한 차이가 나타났는데, 대체적으로 고체중 순계들의 스트레스 지표가 상대적으로 높았으며, 저체중 교잡계들의 스트레스 지표가 낮게 나타났다. 텔로미어 함량과 생산능력 간의 상관성은 20주령 이후부터 유의한 상관관계를 보였는데, 성장능력과는 약한 부(-)의 상관 양상을, 산란능력 및 생존율과는 약한 정(+)의 상관관계를 나타내었다. HSPs 유전자 발현율과 생산능력과의 상관에서 생존율 및 산란지수와는 저도 및 중도의 부의 상관을 보였고, 체중과는 약한 정의 상관관계를 나타내었다. DNA 손상율 분석 또한 체중과는 약한 정의 상관을, 산란지수 및 생존율과는 중도 및 저도의 부의 상관관계를 나타내었다. 이는 체중이 증가할수록 HSPs 유전자 발현율, DNA 손상율 및 텔로미어 감축량이 증가함을 의미하고, 반면 산란능력 및 생존율이 높을수록 HSPs 유전자 발현율과 DNA 손상율은 낮아지고, 텔로미어 함량은 상대적으로 높다는 것을 시사한다. 스트레스 표지 인자들 간의 상호 관계에서 모든 표지 값 간에 유의한 상관관계가 나타났는데, HSPs 발현값과 텔로미어 함량과는 부의 상관을, DNA 손상율과는 정의 상관을 보이고, 텔로미어 함량과 DNA 손상율과는 높은 부의 상관을 나타내었다. 이상의 스트레스 표지 값과 생산능력 간의 상관분석 결과에 따라 닭에 있어 HSPs 및 DNA 손상율의 증가는 텔로미어 감축을 촉진하고, 이에 따라 개체의 항병성 및 강건성을 저하시켜 산란능력과 생존율에 나쁜 영향을 미치는 것으로 판단된다.

This study was conducted to verify the relationships between the expression values of stress-related markers and their production performances in 25 strains of Korean domestic chicken breeds. For stress response markers, the amount of telomeric DNA; expression levels of heat shock protein (HSP)-70, $HSP-90{\alpha}$, and $HSP-90{\beta}$; and comet scores were analyzed. Production performances were measured by the survival rate, body weights, days at first egg laying, egg weight and hen housed egg production. The results showed that the production traits and values of stress-related markers showed significant differences between strains. In general, the stress response of pure bred chickens with heavy weights was relatively high, while that of hybrid chickens with light weights was relatively low. The correlation coefficients between telomere contents and body weights showed that there were weak negative relationships. However, the correlations of telomere content with the survival rate and egg production were weakly positive after 20 weeks old. The expression levels of HSP genes and DNA damage rate (comet scores) were positively correlated to body weight, but were negatively correlated to the survival rate and egg production. The results implied that increasing body weight was associated with increasing HSPs expression and the DNA damage rate was associated with decreasing telomere content. In addition, increasing HSPs expression and the DNA damage rate decreased the survival rate and egg production, but the relationships with the telomere content was the reverse. Correlations among the stress-related markers showed that there were significant correlation coefficients between all of the marker values. HSPs expression was negatively correlated to the telomere content, while it was positively correlated to the DNA damage rate. There was a highly negative correlation between the telomere content and DNA damage rate. In conclusion, increasing the HSP values and DNA damage rate can promote telomere reduction, which led to a decrease in disease resistance and robustness of the chicken. Thus, increasing the stress response was verified to adversely affect the laying performance and viability of chickens.

키워드

참고문헌

  1. Al-Fataftah AR, Abu-Dieyeh ZHM 2007 Effect of chronic heat stress on broiler performance in Jordan. Int J Poul Sci 6(1):64-70. https://doi.org/10.3923/ijps.2007.64.70
  2. Baos R, Jovani R, Pastor N, Tella JL, Jimenez B, Gomez G, Gonzalez MJ, Hiraldo F 2006 Evaluation of genotoxic effects of heavy metals and arsenic in wild nestling white storks (Ciconia ciconia) and black kites (Milvus migrans) from southwestern Spain after a mining accident. Environ Toxicol Chem 25(10):2794-2803. https://doi.org/10.1897/05-570R.1
  3. Beloor J, Kang HK, Kim YJ, Subramani VK, Jang IS, Sohn SH, Moon YS 2010 The effect of stocking density on stress related genes and telomeric length in broiler chickens. Asian-Aust J Anim Sci 23:437-443. https://doi.org/10.5713/ajas.2010.90400
  4. Beuving G, Vonder GM 1978 Effect of stressing factors on corticosterone levels in the plasma of laying hens. Gen Comp Endocrinol 35(2):153-159. https://doi.org/10.1016/0016-6480(78)90157-0
  5. Bobek S, Niezgoda J, Pietras M, Kacinska M, Ewy Z 1980 The effect of acute cold and warm ambient temperatures on the thyroid hormone concentration in blood plasma, blood supply, and oxygen consumption in Japanese quail. Gen Comp Endocrinol 40:201-210. https://doi.org/10.1016/0016-6480(80)90123-9
  6. Cahaner A, Ajuh JA, Siegmund-Schultze M, Azoulay Y, Druyan S, Zarate AV 2008 Effects of the genetically reduced feather coverage in naked neck and featherless broilers on their performance under hot conditions. Poult Sci 87(12):2517-2527. https://doi.org/10.3382/ps.2008-00284
  7. Chen JH, Hales CN, Ozanne SE 2007 DNA damage, cellular senescence and organismal ageing: Causal or correlative? Nucleic Acids Res 35:7417-7428. https://doi.org/10.1093/nar/gkm681
  8. Cho EJ, Park JA, Choi ES, Sohn SH 2016 Comparison of stress response in diallel crossed Korean domestic chicken breeds. Korean J Poult Sci 43(2):77-88. https://doi.org/10.5536/KJPS.2016.43.2.77
  9. Cottliar AS, Slavutsky IR 2001 Telomeres and telomerase activity: Their role in aging and in neoplastic development. Medicina 61:335-342.
  10. Davis GS, Siopes TD 1987 Plasma corticosterone response of turkeys to adrenocorticotropic hormone: Age, dose, and route of administration effects. Poult Sci 66(10):1727-1732. https://doi.org/10.3382/ps.0661727
  11. Delezie E, Swennen Q, Buyse J, Decuypere E 2007 The effect of feed withdrawal and crating density in transit on metabolism and meat quality of broilers at slaughter weight. Poult Sci 86:1414-1423. https://doi.org/10.1093/ps/86.7.1414
  12. Davis GS, Anderson KE, Carroll AS 2000 The effects of longterm caging and molt of Single Comb White Leghorn hens on heterophil to lymphocyte ratios, corticosterone and thyroid hormones. Poult Sci 79(4):514-518. https://doi.org/10.1093/ps/79.4.514
  13. Elnagar SA, Scheideler SE, Beck MM 2010 Reproductive hormones, hepatic deiodinase messenger ribonucleic acid, and vasoactive intestinal polypeptide-immunoreactive cells in hypothalamus in the heat stress-induced or chemically induced hypothyroid laying hen. Poult Sci 89:2001-2009. https://doi.org/10.3382/ps.2010-00728
  14. Faullimel C, Ennahar S, Aoude-Werner D, Guterl P, Marchioni E 2005 DNA comet assay for the detection of time-temperature abuse during the storage of poultry. J Food Prot 68(7):1414-1420. https://doi.org/10.4315/0362-028X-68.7.1414
  15. Felver-Gant JN, Mack LA, Dennis RL, Eicher SD, Cheng HW 2012 Genetic variations alter physiological responses following heat stress in 2 strains of laying hens. Poult Sci 91(7):1542-1551. https://doi.org/10.3382/ps.2011-01988
  16. Frankic T, Pajk T, Rezar V, Levart A, Salobir J 2006 The role of dietary nucleotides in reduction of DNA damage induced by T-2 toxin and deoxynivalenol in chicken leukocytes. Food Chem Toxicol 44(11):1838-1844. https://doi.org/10.1016/j.fct.2006.06.002
  17. Garriga C, Hunter RR, Amat C, Planas JM, Mitchell MA, Moreto M 2006 Heat stress increases apical glucose transport in the chicken jejunum. Am J Physiol Reg Integ Comp Physiol 290:R195-R201. https://doi.org/10.1152/ajpregu.00393.2005
  18. Geraert PA, Padilha JC, Guillaumin S 1996 Metabolic and endocrine changes induced by chronic heat exposure in broiler chicken: Biological and endocrinological variables. Br J Nutr 75:205-216.
  19. Gornati R, Papis E, Simona R, Genciana T, Marco S, Giovanni B 2004 Rearing density influences the expression of stressrelated genes in sea bass (Dicentrarchus labrax, L.). Gene 341:111-118. https://doi.org/10.1016/j.gene.2004.06.020
  20. Gross WB 1989 Factors affecting chicken thrombocyte morphology and the relationship with heterophil:lymphocyte ratios. Br Poult Sci 30(4):919-925. https://doi.org/10.1080/00071668908417218
  21. Gross WB, Siegel HS 1983 Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis 27(4):972-979. https://doi.org/10.2307/1590198
  22. Imik H, Ozlu H, Gumus R, Atasever MA, Urcarand S, Atasever M 2012 Effect of ascorbic acid and $\alpha$-lipoic acid on performance and meat quality of broiler subjected to heat stress. Br Poult Sci 53:800-808. https://doi.org/10.1080/00071668.2012.740615
  23. Jang IS, Ko YH, Moon YS, Sohn SH 2014 Effects of vitamin C or E on the pro-inflammatory cytokines, heat shock protein 70 and antioxidant status in broiler chicks under summer conditions. Asian-Aust J Anim Sci 27(5):749-756.
  24. Kamboh AA, Hang SQ, Bakhetgul M, Zhu WY 2013 Effects of genistein and hesperidin on biomarkers of heat stress in broilers under persistent summer stress. Poult Sci 92(9): 2411-2418. https://doi.org/10.3382/ps.2012-02960
  25. Kregel KC 2002 Heat shock proteins: Modifying factors in physiological stress responses and acquired thermotolerance. J Appl Physiol 92(5):2177-2186. https://doi.org/10.1152/japplphysiol.01267.2001
  26. Lay DC Jr, Fulton RM, Hester PY, Karcher DM, Kjaer JB, Mench JA, Mullens BA, Newberry RC, Nicol CJ, O'Sullivan NP, Porter RE 2011 Hen welfare in different housing systems. Poult Sci 90(1):278-294. https://doi.org/10.3382/ps.2010-00962
  27. Lee RF, Steinert S 1995 Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutat Res 544(1):43-64.
  28. Lew SH, Kim DW, Suh JK, Seung IS, Shim JC, Cheong MA, Park JH 2004 Genotoxicity of enflurance in human periphral blood lymphocytes studied in vivo by Single Cell Gel Electrophoresis. Korean J Anesthesiol 47(2):162-166. https://doi.org/10.4097/kjae.2004.47.2.162
  29. Lin H, Decuypere E, Buyse J 2004 Oxidative stress induced by corticosterone administration in broiler chickens (Gallus gallus domesticus) 2. Short-term effect. Comp Biochem Physiol B Biochem Mol Biol 139(4):745-751. https://doi.org/10.1016/j.cbpc.2004.09.014
  30. Lin H, Buyse J, Decuypere E 2006 Acute heat stress induces oxidative stress in broiler chickens. Comp Biochem Physiol A Integr Physiol 144:11-17. https://doi.org/10.1016/j.cbpa.2006.01.032
  31. Lindquist S 1986 The heat-shock response. Ann Rev Biochem 55:1151-1191. https://doi.org/10.1146/annurev.bi.55.070186.005443
  32. Lindquist S, Craig EA 1988 The heat-shock proteins. Annu Rev Genet 22:631-677. https://doi.org/10.1146/annurev.ge.22.120188.003215
  33. Livak KJ, Schmittgen TD 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402-408. https://doi.org/10.1006/meth.2001.1262
  34. Mader J, Arad Z 1989 Panting and acid-base regulation in heat stressed birds. Comp Biochem Physiol A Comp Physiol 94:395-400. https://doi.org/10.1016/0300-9629(89)90112-6
  35. Mashaly MM, Hendricks GL 3rd, Kalama MA, Gehad AE, Abbas AO, Patterson PH 2004 Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult Sci 83(6):889-894. https://doi.org/10.1093/ps/83.6.889
  36. Maxwell MH 1993 Avian blood leucocyte responses to stress. World's Poult Sci J 49:34-43. https://doi.org/10.1079/WPS19930004
  37. Meeker AK, Coffey DS 1997 Telomerase: A promising marker of biological immortality of germ, stem, and cancer cells. Biochemistry 62:1323-1331.
  38. Muiruri HK, Harrison PC 1991 Effect of roost temperature on performance of chickens in hot ambient environments. Poult Sci 70(11):2253-2258. https://doi.org/10.3382/ps.0702253
  39. Mujahid A, Pumford NR, Bottje W, Nakagawa K, Miyazawa T, Akiba Y, Toyomizu M 2007 Mitochondrial oxidative damage in chicken skeletal muscle induced by acute heat stress. Jpn Poult Sci 44:439-445. https://doi.org/10.2141/jpsa.44.439
  40. Mumma JO, Thaxton JP, Vizzier-Thaxton Y, Dodson WL 2006 Physiological stress in laying hens. Poult Sci 85(4):761-769. https://doi.org/10.1093/ps/85.4.761
  41. Mustaf S, Kahraman NS, Firat MZ 2009 Intermittent partial surface wetting and its effect on body-surface temperatures and egg production of white brown domestic laying hens in Antalya(Turkey) Br Poult Sci 50:30-38.
  42. Njoya J, Picard M 1994 Climatic adaptation of laying hens. Trop Anim Health Prod 26(3):180-186. https://doi.org/10.1007/BF02241082
  43. Novero RP, Beck MM, Gleaves EW, Johnson AL, Deshazer JA 1991 Plasma progesterone, luteinizing hormone concentration, and granulose cell responsiveness in heat-stressed hens. Poult Sci 70:2335-2339. https://doi.org/10.3382/ps.0702335
  44. Powers MV, Workman P 2007 Inhibitors of the heat shock response: Biology and pharmacology. FEBS Lett 581(19): 3758-3769. https://doi.org/10.1016/j.febslet.2007.05.040
  45. Quintero-Filho WM, Ribeiro A, Ferraz-de-Paula V, Pinheiro ML, Sakai M, Sa LRM, Ferreira AJP, Palermo-Neto J 2010 Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens. Poult Sci 89:1905-1914. https://doi.org/10.3382/ps.2010-00812
  46. Reece FN, Deaton JW, Kubena LF 1972 Effects of high temperature and humidity on heat prostration of broiler chickens. Poult Sci 51:2021-2025. https://doi.org/10.3382/ps.0512021
  47. Richter T, Proctor C 2007 The role of intracellular peroxide levels on the development and maintenance of telomeredependent senescence. Exp Gerontol 42:1043-1052. https://doi.org/10.1016/j.exger.2007.08.004
  48. Rimoldi S, Lasagna E, Sarti FM, Marelli SP, Cozzi MC, Bernardini G, Terova G 2015 Expression profile of six stressrelated genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions. Meta Gene 31(6):17-25.
  49. Ritossa FM 1962 A new puffing pattern induced by temperature shock and DNP in Drosphila. Experientia 18:571-573. https://doi.org/10.1007/BF02172188
  50. Rozenboim I, Tako E, Gal-Garber O, Proudman JA, Uni Z 2007 The effect of heat stress on ovarian function of laying hens. Poult Sci 86:1760-1765. https://doi.org/10.1093/ps/86.8.1760
  51. Ryu JC, Kim HJ, Seo YR, Kim KR 1997 Single cell gel electrophoresis (comet assay) to detect DNA damage and apoptosis in cell level. Environ Mutagens Carcinogens 17: 71-77.
  52. Sandercock DA, Hunter RR, Mitchell MA, Hocking PM 2006 Thermoregulatory capacity and muscle membrane integrity are compromised in broilers compared with layers at the same age or body weight. Br Poult Sci 47:322-329. https://doi.org/10.1080/00071660600732346
  53. Scanes CG 2016 Biology of stress in poultry with emphasis on glucocorticoids and the heterophil to lymphocyte ratio. Poult Sci doi:10.3382
  54. Singh NP, McCoy MT, Tice RR, Schneider EL 1988 A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175(1):184-191. https://doi.org/10.1016/0014-4827(88)90265-0
  55. Slijepcevic P 2001 Telomere length measurement by Q-FISH. Cell Sci 23:14-22.
  56. Sohn SH, Cho EJ, Park DB, Jang IS, Moon YS 2014 Comparison of stress response between Korean Native Chickens and Single Comb White Leghorns subjected to a high stocking density. Korean J Poult Sci 41(2):115-125. https://doi.org/10.5536/KJPS.2014.41.2.115
  57. Sohn SH, Cho EJ, Park JA, Hong YH, Kim CD 2015 Analysis of stress response of domestic chicken breeds for the development of a new synthetic parent stock. Korean J Poult Sci 42(2):157-167. https://doi.org/10.5536/KJPS.2015.42.2.157
  58. Sohn SH, Jang IS, Son BR 2011 Effect of housing systems of cage and floor on the production performance and stress response in layer. Korean J Poult Sci 38(4):305-313. https://doi.org/10.5536/KJPS.2011.38.4.305
  59. Sohn SH, Subramani VK 2014 Dynamics of telomere length in the chicken. World's Poult Sci J 70:721-735. https://doi.org/10.1017/S0043933914000804
  60. Sohn SH, Subramani VK, Moon YS, Jang IS 2012 Telomeric DNA quantity, DNA damage, and heat shock protein gene expression as physiological stress markers in chickens. Poult Sci. 91(4):829-836. https://doi.org/10.3382/ps.2011-01904
  61. Soleimani AF, Zulkifli I, Omar AR, Raha AR 2011 Physiological responses of 3 chicken breeds to acute heat stress. Poult Sci 90(7):1435-1440. https://doi.org/10.3382/ps.2011-01381
  62. Song Z, Liu L, Sheikhahmadi A, Jiao H, Lin H 2012 Effect of heat exposure on gene expression of feed intake regulatory peptides in laying hens. J Biomed Biotechnol 2012: 1-8.
  63. Star L, Decuypere E, Parmentier HK, Kemp B 2008a Effect of single or combined climatic and hygienic stress in four layer lines: 2. Endocrine and oxidative stress responses. Poult Sci 87(6):1031-1038. https://doi.org/10.3382/ps.2007-00143
  64. Star L, Kemp B, van den Anker I, Parmentier HK 2008b Effect of single or combined climatic and hygienic stress in four layer lines: 1. Performance. Poult Sci 87(6):1022-1030. https://doi.org/10.3382/ps.2007-00142
  65. Tamzil MH, Noor RR, Hardjosworo PS, Manalu W, Sumantri C 2013 Acute heat stress responses of three lines of chickens with different heat shock protein(HSP)-70 genotypes. Int J Poult Sci 12(5):264-272. https://doi.org/10.3923/ijps.2013.264.272
  66. Wang R, Pan XJ, Peng ZQ 2009 Effects of heat exposure on muscle oxidant and protein functionalities of pectoralis majors in broilers. Poult Sci 88:1078-1084. https://doi.org/10.3382/ps.2008-00094
  67. Woelfel RL, Owens CM, Hirschler EM, Martinez-Dawson R, Sams AR 2002 The chracterization and incidence of pale, soft, and exudative broiler meat in a commercial processing plant. Poult Sci 81(4):579-584. https://doi.org/10.1093/ps/81.4.579
  68. Yahav S, Luger D, Cahaner A, Dotan M, Rusal M, Hurwitz S 1998 Thermoregulation in naked neck chickens subjected to different ambient temperatures. Br Poult Sci 39(1):133-138. https://doi.org/10.1080/00071669889510
  69. Young JC, Moarefi I, Hartl FU 2001 Hsp90: a specialized but essential protein-folding tool. J Cell Biol 154(2):267-273. https://doi.org/10.1083/jcb.200104079
  70. Zulkifli I, Najafi P, Nurfarahin AJ, Soleimani AF, Kumari S, Aryani AA, O'Reilly EL, Eckersall PD 2014 Acute phase proteins, interleukin 6, and heat shock protein 70 in broiler chickens administered with corticosterone. Poult Sci 93(12)3112-3118. https://doi.org/10.3382/ps.2014-04099
  71. Zulkifli I, Siegel HS, Mashaly MM, Dunnington EA, Siegel PB 1995 Inhibition of adrenal steroidogenesis, neonatal feed restriction and pituitary-adrenal axis response to subsequent fasting in chickens. Gen Comp Endocrinol 97(1):49-56. https://doi.org/10.1006/gcen.1995.1005