Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Simultaneous Determination and Recognition Analysis of Coumarins in Angelica decursiva and Peucedanum praeruptorum by HPLC-DAD

  • Kim, Hye Mi (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Jeong, Su Yang (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kim, Sun Min (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Lee, Kyu Ha (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kim, Jong Hwan (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Seong, Rack Seon (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
  • Received : 2015.10.26
  • Accepted : 2016.02.22
  • Published : 2016.09.30
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Abstract

Peucedani Radix is the root of Angelica decursiva Franchet et Savatier (=Peucedanum decursivum Maximowicz) or Peucedanum praeruptorum Dunn in several Asian countries. The coumarins contained in Peucedani Radix were quantitatively analyzed using HPLC-DAD to develop a simultaneous determination for the quality control of A. decursiva and P. praeruptorum. For quantitative analysis, four major coumarins contained in these medicinal plants were assessed. Nodakenin (1), nodakenetin (2), praeruptorin A (3), and praeruptorin B (4) were separated with a Phenomenex Luna C18 column ($5{\mu}m$, $4.6{\times}250mm$) under the gradient conditions using distilled water with 0.1% phosphoric acid and acetonitrile with 0.1% phosphoric acid as the mobile phase, at a flow rate of 1.0 ml/min and a detection wavelength of 330 nm. This method was fully validated for linearity, accuracy, precision, recovery, and limit of detection and quantification. As a result, A. decursiva and P. praeruptorum were clearly classified by the quantification of four major coumarins in extracts. Also, the pattern recognition analysis based on HPLC indicates that all of the samples were largely clustered into two groups. Therefore, it is possible to distinguish between A. decursiva and P. praeruptorum and contribute to quality control.

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References

  1. Miyazawa, M.; Shimamura, H.; Bhuva, R. C.; Nakamura, S. I.; Kameoka, H. J. Agric. Food Chem. 1996, 44, 3444-3448. https://doi.org/10.1021/jf960025a
  2. Zhao, D.; Islam, M. N.; Ahn, B. R.; Jung, H. A.; Kim, B. W.; Choi, J. S. Arch. Pharm. Res. 2012, 35, 179-192. https://doi.org/10.1007/s12272-012-0120-0
  3. Korea Food and Drug Administration, The Korean Herbal Pharmacopoeia 4th ed. (KHP), 2013, p 316.
  4. The Ministry of Health, Labour and Welfare. Dissolution Test. The Japanese Pharmacopoeia, 16th ed. (J.P.), The Ministry of Health, Labour and Welfare, Tokyo, 2011, pp 1706-1707.
  5. Pharmacopoeia Commission of the People's Republic of China (ChP). Pharmacopoeia of the People's Republic of China 2010. Beijing, China Medical Science and Technology Press, 2010, pp 318-320.
  6. Hata, K.; Sano, K. Tetrahedron Lett. 1966, 7, 1461-1465. https://doi.org/10.1016/S0040-4039(00)75716-9
  7. Liu, R.; Sun, Q.; Shi, Y.; Kong, L. J. Chromatogr. A. 2005, 1076, 127-132. https://doi.org/10.1016/j.chroma.2005.04.041
  8. Hou, Z.; Xu, D.; Yao, J.; Luo, J.; Kong, L.; J. Chromatogr. B. 2009, 877, 2571-2578. https://doi.org/10.1016/j.jchromb.2009.06.042
  9. Hou, Z.; Luo, J.; Wang, J.; Kong, L. Sep. Purif. Technol. 2010, 75, 132-137. https://doi.org/10.1016/j.seppur.2010.08.007
  10. Kong, L.Y.; Li, Y.; Min, Z. D.; Li, X.; Zhu, T. R. Phytochemistry. 1996, 41, 1423-1426. https://doi.org/10.1016/0031-9422(95)00783-0
  11. Islam, M. N.; Choi, R. J.; Jin, S. E.; Kim, Y. S.; Ahn, B. R.; Zao, D.; Jung, H. A. Choi, J. S. J. Ethnopharmacol. 2012, 144, 175-181. https://doi.org/10.1016/j.jep.2012.08.048
  12. Lee, G. S.; Doh, E. J.; Jeong, S. I.; Kim, H. J.; Lee, J. C.; Oh, S. E.; Ju, Y. S.; Cho, S. I. Kor. J. Herbology. 2011, 26, 57-63.
  13. Choo, B. K.; Moon, B. C.; Ji, Y.; Kim, B. B.; Choi, G.; Yoon, T.; Kim, H. K. Biol. Pharm. Bull. 2009, 32, 24-30. https://doi.org/10.1248/bpb.32.24
  14. Liu, R.; Feng, L.; Sun, A.; Kong, L. J. Chromatogr. A. 2004, 1057, 89-94. https://doi.org/10.1016/j.chroma.2004.09.047
  15. Jeong, S. I.; Kim, J. H. Kor. Herb. Med. Inf. 2013, 1.

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