한국토양비료학회지 (Korean Journal of Soil Science and Fertilizer)

  • 제49권3호
  • /
  • Pages.251-258
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  • 2016
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  • 0367-6315(pISSN)
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  • 2288-2162(eISSN)
한국토양비료학회 (Korean Society of Soil Science and Fertilizer)
권호 표지
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Plant Growth Promotion and Antagonistic Activities Against Anthracnose of Burkholderia sp. LPN-2 Strain

  • Kim, WonChan (School of Applied of Life Science, Kyungpook National University) ;
  • Seo, SangHyun (Research institute, DaeWon Chemical Inc.) ;
  • Lee, ChangHee (School of Applied of Life Science, Kyungpook National University) ;
  • Park, JunHong (Gyeongsangbuk-Do Agricultural Research and Extension Services) ;
  • Kang, SangJae (School of Applied of Life Science, Kyungpook National University)
  • 투고 : 2016.04.01
  • 심사 : 2016.06.14
  • 발행 : 2016.06.30
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초록

A rhizobacterium LPN-2, which showed strong antifungal activity and auxin producing ability, was isolated from a farmland in North Gyeongsang Province, South Korea. Based on analysis of the 16S rDNA sequence, strain LPN-2 was identified as a novel strain of Burkholderia and was designated as Burkholderia sp. LPN-2. In vitro experiments showed that the isolated stain LPN-2 significantly produced auxin within 48 hr incubation. In order to check for PGPR function we performed in vivo growth promoting test in different crops, including mung bean, pea and cabbage. Application of Burkholderia sp. LPN-2 showed dramatic growth promoting effect on all the tested plants. We also confirmed siderophore and cellulase productions by Burkholderia sp. LPN-2 using CAS blue agar and CMC plate test. Further treatment with LPN-2 and the crude culture broth was effective in suppressing anthracnose in vitro test and also reduced incidence and severity of anthracnose in apple and pepper. Taken together, we conclude that Burkholderia sp. LPN-2 might be used as organic fertilizer for effective crop production in organic farming.

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