한국해양환경ㆍ에너지학회지 (Journal of the Korean Society for Marine Environment & Energy)

  • 제19권3호
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  • Pages.236-245
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  • 2016
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  • 2288-0089(pISSN)
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  • 2288-081X(eISSN)
한국해양환경•에너지학회 (The Korean Society for Marine Environment and Energy)
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염분과 온도의 동시 영향에 따른 해양 미세조류 Nannochloropsis granulata와 Chlorella vulgaris의 중성지질 및 녹말 축적에 관한 연구

Simultaneous Effect of Salinity and Temperature on the Neutral Lipid and Starch Accumulation by Oceanic Microalgae Nannochloropsis granulata and Chlorella vulgaris

  • 고경준 (제주대학교 해양생명과학과) ;
  • 이치헌 (제주대학교 해양생명과학과) ;
  • 문혜나 (제주대학교 해양생명과학과) ;
  • 이연지 (제주대학교 해양생명과학과) ;
  • 양진주 (대한피부과학연구소) ;
  • 조기철 (한국기초과학지원연구원 제주센터) ;
  • 김대경 (한국기초과학지원연구원 제주센터) ;
  • 여인규 (제주대학교 해양생명과학과)
  • Ko, Kyungjun (Department of Marine life Science, Jeju National University) ;
  • Lee, Chi-Heon (Department of Marine life Science, Jeju National University) ;
  • Moon, Hye-Na (Department of Marine life Science, Jeju National University) ;
  • Lee, Yeon-Ji (Department of Marine life Science, Jeju National University) ;
  • Yang, Jinju (Korea Dermatology Research Institute) ;
  • Cho, Kichul (Jeju Center, Korea Basic Science Institute (KBSI)) ;
  • Kim, Daekyung (Jeju Center, Korea Basic Science Institute (KBSI)) ;
  • Yeo, In-Kyu (Department of Marine life Science, Jeju National University)
  • 투고 : 2016.06.29
  • 심사 : 2016.08.05
  • 발행 : 2016.08.25
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초록

미세조류는 육상 식물과 비교하여 높은 성장률을 나타내며 다량의 지질과 탄수화물을 축적할 뿐 아니라 카로테노이드, 폴리페놀과 같은 생리활성 물질들을 체내에 축적하므로 바이오 에너지 및 기타 산업의 유망한 재료로 인식 되어왔다. 미세조류의 온도, 염분, 빛 등 비생물적 스트레스와 다양한 배양 조건에 따른 생화학 물질의 축적 변화 양상에 대한 연구는 많이 진행되어 왔지만 그러한 조건들의 동시적인 효과에 따른 성장과 생화학물질 조성 변화에 대한 연구는 거의 진행되지 않았다. 따라서 이번 연구에서는 산업적으로 많이 활용되고 있는 두 해양 미세조류인 Chlorella vulgaris와 Nannochloropsis granulata의 염분(10, 30, 50 psu) 및 온도(20, 25, $30^{\circ}C$)의 동시 배양 조건에 따른 바이오 매스의 변화와 바이오 에너지에 사용되는 중성지질 및 녹말의 축적 변화를 회분배양의 실험적 조건에서 측정하였다. 그 결과 $30^{\circ}C$, 30 psu 조건에서 C. vulgaris 및 N. granulata 모두 가장 높은 성장을 나타냈고, 광합성 색소인 chlorophyll a 및 carotenoid의 축적 양상이 온도 의존적으로 증가하였으며 중성지질과 녹말의 축적은 염분과 온도의 조합에 따라 두 종의 양상이 서로 다르게 나타나는 사실을 확인할 수 있었다. 이를 통해 미세조류의 염분에 의한 성장과 중성지질 및 녹말의 축적 양상은 서로 다른 온도 조건에 따라 그 변화 정도가 다르게 나타날 수 있다는 사실을 확인할 수 있었다.

Because microalgae represent high growth rate than terrestrial plants, and it can accumulate significant lipid and carbohydrate content, and other bioactive compounds such as carotenoid and polyphenol in their body, it has been considered as one of the promising resources in bio-energy, and other industries. Although many studies has been performed about the microalgae-derived biochemical accumulation under various abiotic conditions such as different temperatures, salinities and light intensities, the studies about simultaneous effect of those parameters has rarely been performed. Therefore, this study focused on evaluation of simultaneous effect of different salinity (10, 30, 50 psu) and temperatures (20, 25, $30^{\circ}C$) on the changes of biomass, lipid, starch and photosynthetic pigment accumulation. As results, the highest growth rate was achieved at $30^{\circ}C$ and 30 psu in the both algal cultures, and the photosynthetic pigment, chlorophyll a and total carotenoid content, were increased in a temperature-dependent manner. The accumulation of lipid and starch contents exhibited different aspects under different combinations of temperature and salinity. From the results, it is suggested that the changes of microalgal lipid and starch accumulation under different salinities may be affected by the different temperatures.

키워드

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