DOI QR코드

DOI QR Code

Combined Treatment of Chlorine Dioxide Gas, Mild Heat, and Fumaric Acid on Inactivation of Listeria monocytogenes and Quality of Citrus unshiu Marc. during Storage

이산화염소 가스 훈증, 중온 열수 및 푸마르산 병합처리가 감귤의 미생물학적 안전성 및 저장 중 품질에 미치는 영향

  • Kim, Hyun Gyu (Department of Food Science and Technology, Chungnam National University) ;
  • Min, Sea Cheol (Department of Food Science and Technology, Seoul Women's University) ;
  • Oh, Deog Hwan (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Koo, Ja Jun (Eco Biotech) ;
  • Song, Kyung Bin (Department of Food Science and Technology, Chungnam National University)
  • Received : 2016.04.11
  • Accepted : 2016.05.24
  • Published : 2016.08.31

Abstract

Combined treatment of chlorine dioxide ($ClO_2$) gas, mild heat, and fumaric acid was performed to reduce microbial growth and maintain quality of Citrus unshiu during storage at $4^{\circ}C$. Citrus unshiu fruits were treated with $ClO_2$ gas (15 or 30 ppmv), mild heat (40, 50, or $60^{\circ}C$), and fumaric acid (0.1, 0.3, or 0.5%). Combined treatment of 15 or 30 ppmv $ClO_2$ gas, $50^{\circ}C$ mild heat, and 0.5% fumaric acid reduced populations of inoculated Listeria monocytogenes by 3.5~3.7 log CFU/g. In addition, combined treatment decreased populations of yeast and molds in Citrus unshiu by 2.54 log CFU/g after 30-day storage at $4^{\circ}C$. Combined treatment also reduced the decay rate by 48% after 30 days of storage compared with the control. Total solid content, titratable acidity, and color values were not significantly affected by the combined treatment. Therefore, combined treatment of $ClO_2$ gas, mild heat, and fumaric acid can be a useful hurdle technology to improve microbial safety and quality of Citrus unshiu during storage.

감귤의 저장 중 미생물학적 안전성 확보 및 품질 향상을 위해 0.5% 푸마르산과 $50^{\circ}C$ 열수 세척처리 후 15, 30 ppmv 이산화염소 가스 훈증 처리하여 $4{\pm}1^{\circ}C$에서 30일간 저장하였다. 푸마르산, 열수 세척처리, 15와 30 ppmv 병합처리 시 접종된 Listeria monocytogenes를 3.5~3.7 log CFU/g 감소시켰다. 또한, 감귤의 병합처리 후 30 ppmv 병합처리구에서 저장 30일 후에 효모 및 곰팡이 수에 있어서 2.54 log CFU/g의 감소 효과를 나타냈고, 대조구와 비교하여 부패율을 48% 낮추는 효과를 보였다. 저장 중 당도, 산도는 대조구와 비교하여 유의적인 차이를 나타내지 않았고, 병합처리는 감귤 시료의 표면 색도에 부정적인 영향을 끼치지 않았다. 따라서 본 연구 결과 이산화염소 가스, 중온 열수 세척, 푸마르산 병합처리는 감귤 시료의 품질에 영향을 끼치지 않으면서 미생물학적 안전성 및 품질을 향상할 수 있는 효과적인 허들기술이라고 판단된다.

Keywords

References

  1. Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC. 2004. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J Agric Food Chem 52: 3389-3393. https://doi.org/10.1021/jf049899k
  2. Lee HH, Hong SI, Son SM, Kim DM. 2011. Effect of onsite postharvest hot water treatment on storage quality of commercial greenhouse satsuma mandarin. Korean J Food Sci Technol 43: 577-582. https://doi.org/10.9721/KJFST.2011.43.5.577
  3. Korea Agricultural Marketing Information Service. http://www.kamis.co.kr/customer/circulation/domestic/item.do (accessed Feb 2016).
  4. Nongsaro. http://www.nongsaro.go.kr/portal/ps/pst/psta/trendYearStats.ps?menuId=PS00211&categoryCode=C0300&subCode=1#chartTab (accessed Feb 2016).
  5. Sharma RR, Singh D, Singh R. 2009. Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A review. Biol Control 50: 205-221. https://doi.org/10.1016/j.biocontrol.2009.05.001
  6. Weis J, Seeliger HPR. 1975. Incidence of Listeria monocytogenes in nature. Appl Environ Microbiol 30: 29-32.
  7. Centers for Disease Control and Prevention. http://www.cdc.gov/listeria/outbreaks/index.html (accessed Feb 2016).
  8. Brown GE, Dezman DJ. 1990. Uptake of imazalil by citrus fruit after postharvest application and the effect of residue distribution on sporulation of Penicillium digitatum. Plant Dis 74: 927-930. https://doi.org/10.1094/PD-74-0927
  9. Ito Y, Goto T, Oka H, Matsumoto H, Miyazaki Y, Takahashi N, Nakazawa H. 2003. Simple and rapid determination of thiabendazole, imazalil, and o-phenylphenol in citrus fruit using flow-injection electrospray ionization tandem mass spectrometry. J Agric Food Chem 51: 861-866. https://doi.org/10.1021/jf020809q
  10. Palou L, Usall J, Smilanick JL, Aguilar MJ, Vinas I. 2002. Evaluation of food additives and low-toxicity compounds as alternative chemicals for the control of Penicillium digitatum and Penicillium italicum on citrus fruit. Pest Manag Sci 58: 459-466. https://doi.org/10.1002/ps.477
  11. Lee JH, Seo MW, Kim HG. 2012. Isolation and characterization of an antagonistic endophytic bacterium Bacillus velezensis CB3 the control of citrus green mold pathogen Penicillium digitatum. Korean J Mycol 40: 118-123. https://doi.org/10.4489/KJM.2012.40.2.118
  12. Porat R, Pavoncello D, Peretz J, Weiss B, Daus A, Cohen L, Ben-Yehoshua S, Fallik E, Droby S, Lurie S. 2000. Induction of resistance to Penicillium digitatum and chilling injury in 'Star Ruby' grapefruit by a short hot-water rinse and brushing treatment. J Hortic Sci Biotechnol 75: 428-432. https://doi.org/10.1080/14620316.2000.11511263
  13. Youm HJ, Ko JK, Kim MR, Song KB. 2004. Inhibitory effect of aqueous chlorine dioxide on survival of Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes in pure cell culture. Korean J Food Sci Technol 36: 514-517.
  14. Singh N, Singh RK, Bhunia AK, Stroshine RL. 2002. Efficacy of chlorine dioxide, ozone, and thyme essential oil or a sequential washing in killing Escherichia coli O157:H7 on lettuce and baby carrots. LWT-Food Sci Technol 35: 720-729. https://doi.org/10.1006/fstl.2002.0933
  15. Han Y, Linton RH, Nielsen SS, Nelson PE. 2001. Reduction of Listeria monocytogenes on green peppers (Capsicum annuum L.) by gaseous and aqueous chlorine dioxide and water washing and its growth at $7^{\circ}C$. J Food Prot 64: 1730-1738. https://doi.org/10.4315/0362-028X-64.11.1730
  16. Chun HH, Song KB. 2014. Optimisation of the combined treatments of aqueous chlorine dioxide, fumaric acid and ultraviolet-C for improving the microbial quality and maintaining sensory quality of common buckwheat sprout. Int J Food Sci Technol 49: 121-127. https://doi.org/10.1111/ijfs.12283
  17. Lamikanra O, Bett-Garber KL, Ingram DA, Watson MA. 2005. Use of mild heat pre-treatment for quality retention of fresh-cut cantaloupe melon. J Food Sci 70: C53-C57. https://doi.org/10.1111/j.1365-2621.2005.tb09020.x
  18. Kang JH, Park SM, Kim HG, Son HJ, Song KJ, Cho M, Kim JR, Lee JY, Song KB. 2015. Gaseous chlorine dioxide treatment to produce high quality paprika for export. J Korean Soc Food Sci Nutr 44: 1072-1078. https://doi.org/10.3746/jkfn.2015.44.7.1072
  19. Kondo N, Murata M, Isshiki K. 2006. Efficiency of sodium hypochlorite, fumaric acid, and mild heat in killing native microflora and Escherichia coli O157:H7, Salmonella Typhimurium DT104, and Staphylococcus aureus attached to fresh-cut lettuce. J Food Prot 69: 323-329. https://doi.org/10.4315/0362-028X-69.2.323
  20. Park SH, Kang DH. 2015. Combination treatment of chlorine dioxide gas and aerosolized sanitizer for inactivating foodborne pathogens on spinach leaves and tomatoes. Int J Food Microbiol 207: 103-108. https://doi.org/10.1016/j.ijfoodmicro.2015.04.044
  21. Han Y, Floros JD, Linton RH, Nielsen SS, Nelson PE. 2001. Response surface modeling for the inactivation of Escherichia coli O157:H7 on green peppers (Capsicum annuum L.) by chlorine dioxide gas treatments. J Food Prot 64: 1128-1133. https://doi.org/10.4315/0362-028X-64.8.1128
  22. Chung SK, Lee DS, Koh JS. 1996. Interrelation between respiration rate, peel permeability and internal atmosphere for sealed and wax-coated Satsuma mandarin oranges. Food Biotechnol 5: 330-333.
  23. Lee HH, Hong SI, Son SM, Kim D. 2004. Storage quality of early harvested Satsuma mandarin as influenced by hot air treatment. Korean J Food Preserv 11: 304-312.
  24. Hong SI, Lee HH, Kim D. 2007. Effects of hot water treatment on the storage stability of satsuma mandarin as a postharvest decay control. Postharvest Biol Tec 43: 271-279. https://doi.org/10.1016/j.postharvbio.2006.09.008
  25. Gomez-Lopez VM, Rajkovic A, Ragaert P, Smigic N, Devlieghere F. 2009. Chlorine dioxide for minimally processed produce preservation: a review. Trends Food Sci Tech 20: 17-26. https://doi.org/10.1016/j.tifs.2008.09.005

Cited by

  1. 노지감귤 단기 저장가능성에 대한 경제적 효과 검토 vol.19, pp.8, 2016, https://doi.org/10.5762/kais.2018.19.8.392
  2. Evaluation of Nonthermal Plasma Treatment by Measurement of Stored Citrus Properties vol.43, pp.4, 2016, https://doi.org/10.5307/jbe.2018.43.4.401
  3. Development and Application of Cast Steel Numerical Simulation System for Heat Treatment vol.13, pp.3, 2016, https://doi.org/10.1007/s40962-019-00305-4