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Variation of Phenolics Contents and Antioxidant Activity of Vaccinium oldhamii Miq.

국내 자생 정금나무의 페놀류 함량과 항산화 활성의 다양성

  • Kim, Hyeusoo (Division of Special-purpose Trees, National Institute of Forest Science) ;
  • Lee, Uk (Division of Special-purpose Trees, National Institute of Forest Science) ;
  • Song, Jeong-Ho (Center of Forest Medicinal Resources Research, National Institute of Forest Science) ;
  • Yun, Kyeong Won (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Kim, Sea-Hyun (Division of Special-purpose Trees, National Institute of Forest Science) ;
  • Kim, Moon-Sup (Division of Special-purpose Trees, National Institute of Forest Science)
  • 김혜수 (국립산림과학원 특용자원연구과) ;
  • 이욱 (국립산림과학원 특용자원연구과) ;
  • 송정호 (국립산림과학원 산림약용자원연구소) ;
  • 윤경원 (순천대학교 한약자원개발학과) ;
  • 김세현 (국립산림과학원 특용자원연구과) ;
  • 김문섭 (국립산림과학원 특용자원연구과)
  • Received : 2016.06.01
  • Accepted : 2016.06.16
  • Published : 2016.06.30

Abstract

Vaccinium oldhamii Miq. is a Korean native tree, which is deciduous and shrub tree with broad leaf. Fruits were used primarily for edible or medicinal purposes for bladder infection in Korea and China. However, there is a lack of information on the degree of variation of phytochemical contents and antioxidant activity of these native clonal. We investigated morphological characteristics, phytochemical contents (polyphenol, flavonoid, anthocyanin) and antioxidant activity for the fruits of 24 individual variation from 4 different regions in Korea. The results in terms of horticultural traits and biochemical characteristics showed significant differences among populations and among individuals within population. The total phenolic contents ranged from 4.40 to 10.58 mg GAE/g and the total flavonoid contents ranged from 2.02 to 8.09 mg NE/g. The total anthocyanin contents ranged from 232.5 to 684.3 mg CGE/100g. Reducing power fruits ranged from 0.15 to 1.43 and DPPH free radical scavenging activity ranged from 49.1 to 97.5% at 400 ppm. Results revealed that the region of origin greatly influenced the phytochemical contents and antioxidant activity of V. oldhamii, which might help for the selection and validation of the most productive cultivars in the food and pharmaceutical industries.

우리나라 자생수종인 정금나무는 낙엽활엽관목으로 열매는 식약용으로 사용되어 왔으나 기능성성분 함량 및 항산화 활성에 관한 연구는 미진한 실정이다. 따라서 본 연구는 국내 자생 정금나무 4집단 24개체의 열매에 대한 형태적 특성, 식물생리활성물질(폴리페놀, 플라보노이드, 안토시아닌) 및 항산화활성을 분석하였다. ANOVA 분석결과 모든 특성들에서 집단 및 개체간의 유의적인 차이가 인정되었다. 총 페놀 함량은 4.40~10.58 mg GAE/g, 총 플라보노이드 함량은 2.02~8.09 mg NE/g, 총 안토시아닌 함량은 232.5~684.3 mg CGE/100g, 환원력은 0.15~1.43, DPPH 자유라디컬 소거능은 400 ppm 농도에서 49.1-97.5% 범위를 각각 나타냈다. 특히, 정금나무 열매의 기능성 성분 함량 및 항산화활성이 국내재배 및 북미산 블루베리에 비해 우수한 것으로 나타나 육종소재 및 기능성 성분 소재로서의 이용 가치가 높은 수종으로 판단된다.

Keywords

References

  1. Abeysinghe, D.C., Li, X., Sun, C., Zhang, W., Zhou, C., and Chen, K. 2007. Bioactive compounds and antioxidant capacities in different edible tissues of citrus fruit of four species. Food Chemistry 104: 1338-1344. https://doi.org/10.1016/j.foodchem.2007.01.047
  2. Akhavan, M., Jahangiri, S., and Shafaghar A. 2015. Studies on the antioxidant and antimicrobial activity and flavonoid derivatives from the fruit of Trigonosciadium brachytaenium (Boiss.) Alava. Industrial Crops and Products 63: 114-118. https://doi.org/10.1016/j.indcrop.2014.10.023
  3. Barbosa, A. de P. 2014. Gastroprotective and immunoadjuvant activities of butanolic extract of Calliandra haematocephala. Journal of Medicinal Plant Research 8: 727-730. https://doi.org/10.5897/JMPR2014.5359
  4. Brand-Williams, W., Cuvelier, M.E., and Berset. C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28: 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  5. Candido, T.L.N., Silva, M.R., and Agostini-Costa. 2015. Bioactive compounds and antioxidant capacity of buriti (Mauritia flexuosa L.f.) from the Cerrado and Amazon biomes. Food Chemistry 177: 313-319. https://doi.org/10.1016/j.foodchem.2015.01.041
  6. Chae, J.W., Jo, B.S., Joo, S.H., Ahn, D.H., Chun, S.S., and Cho, Y.J. 2012. Biological and antimicrobial activity of Vaccinium oldhami fruit. Journal of the Korean Society and Food Science and Nutrition 41: 1-6. https://doi.org/10.3746/jkfn.2012.41.1.001
  7. Choi, I.S., Moon, Y.S., and Kwak. E.J. 2012. Composition of resveratrol and other bioactive compounds, and antioxidant activities in different mulberry cultivars. Korean Journal of Horticultural Science and Technology 30: 301-307. https://doi.org/10.7235/hort.2012.12040
  8. Dragovi -Uzelac, V., Savi , Z., Brala, A., Levai, B., Kova evi , D.B., and Bisko, A. 2010. Evaluation of phenolic content and antioxidant capacity of blueberry cultivars (Vaccinium corymbosum L.) grown in the northwest Croatia. Food Technology and Biotechnololy 48: 214-221.
  9. Gu, G.S., Kim, I.H., Jeong, C.H., Kum, D.C., Jang, C.W., Kim, Y.S., Lee, K.Y., and Shim, K.H. 2014. Nutrition components and antioxidative activities of sweet dark cherry (Prunus avium L.) fruits. Journal of Agriculture and Life Science 48: 151-161.
  10. Gulcin, I., Sat, I.G., Beydemir, S., Elmastas, M., and Kufrevioglu. O.I. 2004. Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb.) buds and lavender (Lavandula stoechas L.). Food Chemistry 87: 393-400. https://doi.org/10.1016/j.foodchem.2003.12.008
  11. Hirotoshi, T., Hisato, K., Ryoko, K.T., Kazuo, N., Masao, Y., Haruki, K., and Chizuko Y. 2013. Antioxidant activities and anti-cancer cell proliferation properties of Natsuhaze (Vaccinium oldhamii Miq.), Shashanbo (V. bracteatum Thunb.) and Blueberry cultivars. Plants 2: 57-71. https://doi.org/10.3390/plants2010057
  12. Kang, H.H. 2009. Determination of biological activities of Korean berries and their anthocyanin identification. PhD Diss., Gyeongsang National University, Korea.
  13. Kim, H., Kim, M.S., Kim, S.H., Yun, K.W., and Song, J.H. 2013. Analysis of total phenolic content and antioxidant activity from fruits of Vaccinium oldhamii Miq. Journal of Korean Forestry Society 102: 566-570. https://doi.org/10.14578/jkfs.2013.102.4.566
  14. Kim, M.H., Lee, Y.J., Kim, D.S., and Kim, D.H. 2003. Quality characteristics of fruits dressing. The Journal of Korean Society of Food & Cookery Science 19: 165-173.
  15. Kim, M.S., Kim, S.H., Han, J., and Park, I.H. 2012. Morphological characteristics and classification analysis of selected population of Vaccinium oldhami Miq. Korean Journal of Plant Research 25: 72-79. https://doi.org/10.7732/kjpr.2012.25.1.072
  16. Kim, S.J., Lee, J.J., Lee, S.I., Park, H.W., Lee, J.H., Park, J.S., Kim, S.H., Baek, N.I., Kwon, Y.E., Yang, J.H., Chae, B.S., Lim, J.P., Shin, T.Y., Jeaon, H., Eun, J.S., and Kim. D.K. 2007. Lignan glycosides of Vaccinium oldhami Miquel. Korean Journal of Pharmacognosy 38: 296-298.
  17. Kim, T.C., Bae, K.S., Kim, I.K., and Chun, H.J. 2005. Antioxidative activities of solvent extracts from blueberry. Korean Journal of Oriental physiology and Pathology 19: 179-183.
  18. Kim, T.J. 1996. Korean resources plants III. Seoul National University Korea. pp. 230.
  19. Lee, H.H., Moon, Y.S., Yun, H.K., Park, P.J., and Kwak E.J. 2014. Contents of bioactive constituents and antioxidant activities of cultivated and wild raspberries. Korean Journal of Horticultural Science and Technology 32: 115-122. https://doi.org/10.7235/hort.2014.13114
  20. Lee, J. 2005. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative Study. Journal of AOAC International 88: 1269-1278.
  21. Lee, J.H., Lee, K.T., Yang, J.H., Baek, N.I., and Kim, D.K. 2004. Acetylcholinesterase inhibitors from the Twigs of Vaccinium oldhami Miquel. Archives of Pharmacal Research 27: 53-56. https://doi.org/10.1007/BF02980046
  22. Lee, S.M., You, Y., Kim, K., Park, J., Jeong, C., Jhon, D.Y., and Jun, W. 2012. Antioxidant activities of native Gwongyang Rubus coreanus Miq. Journal of Korean Society of Food Science and Nutrition 41: 327-332. https://doi.org/10.3746/jkfn.2012.41.3.327
  23. Lee, W.G. and Lee, J.A. 2012. Quality characteristics of yogurt dressing prepared with Blueberry juice. The Korean Journal of Culinary Research 18: 255-265.
  24. Lee, Y.N. 1997. Flora of Korea. Kyo-Hak Publishing Co., Seoul, Korea. pp. 590-592.
  25. Lidija, J., Marijan, S., Martina, M.K., and Ivana, N. 2007. Antioxidant activity and polyphenols of Aronia in comparison to other berry species. Agriculturae Conspectus Scientificus 27: 301-306.
  26. Moon, H.K., Lee, S.W., and Kim. J.K. 2013. Physicochemical and quality characteristics of the Korean and American blueberries. Korean Journal of Food Preservation 20: 524-531. https://doi.org/10.11002/kjfp.2013.20.4.524
  27. Nijveldt, R.J., Van, N.E., Van Hoorn, D.E., Boelens, P.G., Van Norren, K., and Van Leeuwen, P.A. 2001. Flavonoids: A review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutrition 74: 418-425. https://doi.org/10.1093/ajcn/74.4.418
  28. Oh, S. and Koh. S.C. 2009. Screening of antioxidative activity and ${\alpha}$-amylase inhibitory activity in angiosperm plants native to Jeju island. Korean Journal of Plant Resources 22: 71-77.
  29. Oyaizu, M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  30. Park, Y., Choi, S.H., Kim, S.H., Jang, Y.S., Han, J., and Chung, H.G. 2008. Functional composition and antioxidant activity from the fruits of Rubus coreanus according to cultivars. Mokchae Konghak 36: 102-109.
  31. Sekizawa, H., Ikuta, K., Mizuta, K., Takechi, S., and Suzutani, T. 2013. Relationship between polyphenol content and anti-influenza viral effects of berries. Journal of the Science of Food and Agriculture 93: 2239-2241. https://doi.org/10.1002/jsfa.6031
  32. Staj i , S.M., Tepi , A.N., Djulas, S.M., Sumi , Z.M., anadanovi -Brunet, J.M., etkovi , G.S., Vuli , J.J., and Tumbas, V.Y. 2012. Chemical composition and antioxidant activity of berry fruits. Acta Periodica Technologica 43: 94-105.
  33. The Korean Nutrition Society. 2011. Phytonutrient nutrition. Life Science Publishing Co, Seoul, Korea. pp. 2-3.
  34. Wang, S.Y., Chen, C.T., Sciarappa, W., Wang, C.Y., and Camp, M.J. 2008. Fruit quality, antioxidant capacity, and flavonoid content of organically and conventionally grown blueberries. Journal of Agricultural and Food Chemistry 56: 5788-5794. https://doi.org/10.1021/jf703775r