Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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LAMP-3 (Lysosome-Associated Membrane Protein 3) Promotes the Intracellular Proliferation of Salmonella typhimurium

  • Lee, Eun-Ju (Department of Biochemistry, College of Medicine, Chungbuk National University) ;
  • Park, Kwan-Sik (Department of Biochemistry, College of Medicine, Chungbuk National University) ;
  • Jeon, In-Sook (Department of Biochemistry, College of Medicine, Chungbuk National University) ;
  • Cho, Jae-Woon (Department of Surgery, College of Medicine, Chungbuk National University) ;
  • Lee, Sang-Jeon (Department of Surgery, College of Medicine, Chungbuk National University) ;
  • Choy, Hyun E. (Department of Microbiology, Chonnam National University Medical School) ;
  • Song, Ki-Duk (Department of Animal Biotechnology, Chonbuk National University) ;
  • Lee, Hak-Kyo (Department of Animal Biotechnology, Chonbuk National University) ;
  • Choi, Joong-Kook (Department of Biochemistry, College of Medicine, Chungbuk National University)
  • Received : 2016.04.26
  • Accepted : 2016.05.11
  • Published : 2016.07.31
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Abstract

Lysosomes are cellular organelles containing diverse classes of catabolic enzymes that are implicated in diverse cellular processes including phagocytosis, autophagy, lipid transport, and aging. Lysosome-associated membrane proteins (LAMP-1 and LAMP-2) are major glycoproteins important for maintaining lysosomal integrity, pH, and catabolism. LAMP-1 and LAMP-2 are constitutively expressed in Salmonella-infected cells and are recruited to Salmonella-containing vacuoles (SCVs) as well as Salmonella- induced filaments (Sifs) that promote the survival and proliferation of the Salmonella. LAMP-3, also known as DC-LAMP/CD208, is a member of the LAMP family of proteins, but its role during Salmonella infection remains unclear. DNA microarray analysis identified LAMP-3 as one of the genes responding to LPS stimulation in THP-1 macrophage cells. Subsequent analyses reveal that LPS and Salmonella induced the expression of LAMP-3 at both the transcriptional and translational levels. Confocal Super resolution N-SIM imaging revealed that LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella. Knockdown of LAMP-3 by specific siRNAs decreased the number of Salmonella recovered from the infected cells. Therefore, we conclude that LAMP-3 is induced by Salmonella infection and recruited to the Salmonella pathogen for intracellular proliferation.

Keywords

References

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