Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Tumorigenicity Evaluation of Umbilical Cord Blood-derived Mesenchymal Stem Cells

  • Park, Sang-Jin (Department of Toxicological Evaluation and Research, Korea Institute of Toxicology) ;
  • Kim, Hyun-Jung (Department of Toxicological Evaluation and Research, Korea Institute of Toxicology) ;
  • Kim, Woojin (Department of Toxicological Evaluation and Research, Korea Institute of Toxicology) ;
  • Kim, Ok-Sun (Department of Toxicological Evaluation and Research, Korea Institute of Toxicology) ;
  • Lee, Sunyeong (Department of Toxicological Evaluation and Research, Korea Institute of Toxicology) ;
  • Han, Su-Yeon (Department of Toxicological Evaluation and Research, Korea Institute of Toxicology) ;
  • Jeong, Eun Ju (Department of Toxicological Evaluation and Research, Korea Institute of Toxicology) ;
  • Park, Hyun-shin (Research Institute, Genexine Co.) ;
  • Kim, Hea-Won (Research Institute, Genexine Co.) ;
  • Moon, Kyoung-Sik (Department of Toxicological Evaluation and Research, Korea Institute of Toxicology)
  • Received : 2015.11.24
  • Accepted : 2016.06.01
  • Published : 2016.07.15
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Abstract

Mesenchymal stem cells (MSCs) have been identified in multiple types of tissue and exhibit characteristic self-renewal and multi-lineage differentiation abilities. However, the possibility of oncogenic transformation after transplantation is concerning. In this study, we investigated the tumorigenic potential of umbilical cord blood-derived MSCs (hUCB-MSCs) relative to MRC-5 and HeLa cells (negative and positive controls, respectively) both in vitro and in vivo. To evaluate tumorigenicity in vitro, anchorage-independent growth was assessed using the soft agar colony formation assay. hUCB-MSCs and MRC-5 cells formed few colonies, while HeLa cells formed a greater number of larger colonies, indicating that hUCB-MSCs and MRC-5 cells do not have anchorage-independent proliferation potential. To detect tumorigenicity in vivo, hUCB-MSCs were implanted as a single subcutaneous injection into BALB/c-nu mice. No tumor formation was observed in mice transplanted with hUCB-MSCs or MRC-5 cells based on macro- and microscopic examinations; however, all mice transplanted with HeLa cells developed tumors that stained positive for a human gene according to immunohistochemical analysis. In conclusion, hUCB-MSCs do not exhibit tumorigenic potential based on in vitro and in vivo assays under our experimental conditions, providing further evidence of their safety for clinical applications.

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References

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