Advances in nano research

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Gadobutrol-dendrimer effects on metastatic and apoptotic gene expression

  • Kebriaezadeh, Abbas (Departments of Toxicology/ Pharmacology and Pharmacoeconomy and Pharmaceutical Management, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Ashrafi, Sepehr (Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Rasouli, Rahimeh (Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Ebrahimi, Seyed Esmaeil Sadat (Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Hamedani, Morteza Pirali (Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Assadi, Artin (Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Saffari, Mostafa (Islamic Azad University Pharmaceutical Sciences Branch (IAUPS)) ;
  • Ardestani, Mehdi Shafiee (Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences)
  • Received : 2016.03.20
  • Accepted : 2016.06.20
  • Published : 2016.06.25
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Abstract

Dendrimers are one of the most appropriate nanocaries for imaging moieties in imaging applications.The purpose of this study was the evalution of cytotoxicity and inducing apoptosis of dendrimers. This study was conducted in order to investigate the metastasis suppression effect of dendrimer in human breast MCF-7 cell line and finding the nanoparticle protein corona in biological enviromental. Dendrimer cytotoxicity effect was assessed by MTT assay. The mRNA experession level of KAI1 as a metastasis suppressor gene, Bax as Pro- apoptotic gene, Bcl-2 as an anti-apoptotic gene and GAPDH as a housekepping gene were determined by real-time PCR assays.concentration-dependent nanoparticle cytotoxicity effect was proofed at range of 1-2 mg/mL in 24 hours, significant upregulation of mRNA expression of Bax, was observed whereas expression of anti-apoptotic Bcl-2 was down-regulated, also expression of metastasis suppressor gene KAI1 was up-regulated. So far a few studies confirmed apoptosis enhancement effect of dendrimers in MCF-7 cell line via bax/bcl-2 pathways. dendrimer nanoparticles was able to act as metastase inhibitor via upregulation of KAI1 gene.

Keywords

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