한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제44권2호
  • /
  • Pages.140-144
  • /
  • 2016
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

사람 장내세균군집 유래 Bacteorides stericoris HJ-15의 Chondroitinase ABC의 클로닝

Cloning of Chondroitinase ABC from Bacteroides stercoris HJ-15, a Human Intestinal Anaerobic Bacterium

  • 투고 : 2015.12.16
  • 심사 : 2016.02.28
  • 발행 : 2016.06.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

사람의 분변으로부터 분리한 Bacteroides stericoris HJ-15로부터 chondroitinase ABC 유전자를 클로닝하였다. 클로닝한 chondroitinase ABC 유전자는 3,090 bp, 1,029 아미노산으로 구성되어 있었다. B. stercoris chondroitinase ABC 유전자는 이미 보고된 chondroitinase ABC 유전자들과 호몰로지가 없었으나, 아미노산서열에서는 82% 호몰로지를 보였다. T7 promoter를 가진 pET-26b+ expression vector에 클로닝한 chondroitinase ABC 유전자를 Escherichia coli BL21 (DE3)에서 발현하여 정제한 재조합 chondroitinase ABC는 chondroitin sulfate A, B 및 C를 모두 분해하였다.

The gene encoding chondroitinase ABC from a genomic library of Bacteroides stercoris HJ-15, which was isolated from human feces, was cloned. The cloned gene consisted of 3,090 bp and was predicted to encode a 1,029−amino-acid protein. The B. stercoris chondroitinase ABC gene was not homologous to other chondroitinase ABC genes; however, its amino acid sequence showed 71% homology to that of Bacteroides thetaiotaomicron. The gene was cloned in the pET-26b+ expression vector and expressed under the T7 promoter in Escherichia coli BL21(DE3). The purified recombinant chondroitinase ABC degraded chondroitin sulfates A, B, and C.

키워드

참고문헌

  1. Ahn MY, Shin KH, Kim DH, Jung EA, Toida T, Linhardt RJ, et al.1998. Characterization of a Bacteroides species from human intestine that degrades glycosaminoglycans. Can. J. Microbiol. 44: 423−429. https://doi.org/10.1139/cjm-44-5-423
  2. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72: 248−254. https://doi.org/10.1016/0003-2697(76)90527-3
  3. Ernst S, Langer R, Cooney CL, Sasisekharan R. 1995. Enzymatic degradation of glycosaminoglycans. Crit. Rev. Biochem. Mol. Biol. 30: 387−444. https://doi.org/10.3109/10409239509083490
  4. Fidler PS, Schuette K, Asher RA, Dobbertin A, Thornton SR, Calle-Patino Y, et al. 1999. Comparing astrocytic cell lines that are inhibitory or permissive for axon growth: the major axon-inhibitory proteoglycan is NG2. J. Neurosci. 19: 8778−8788.
  5. Hong SW, Kim BT, Shin HY, Kim WS, Lee KS, Kim YS, et al. 2002. Purification and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium. Eur. J. Biochem. 269: 2934−2940. https://doi.org/10.1046/j.1432-1033.2002.02967.x
  6. Ingber DE. 1989. Mechanochemical switching between growth and differentiation during fibroblast growth factor-stimulated angiogenesis in vitro: role of extracellular matrix. J. Cell. Biol. 109: 317−330. https://doi.org/10.1083/jcb.109.1.317
  7. Jackson RL, Busch SJ, Cardin AD. 1991. Glycosaminoglycans; molecular properties, protein interactions, and role in physiological processes. Physiol. Rev. 71: 481−539. https://doi.org/10.1152/physrev.1991.71.2.481
  8. Johnson SL, Daligault HE, Davenport KW, Jaissle J, Frey KG, Ladner JT, et al. 2015. Thirty-two complete genome assemblies of nine Yersinia species, including Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica., Genome. Announc. 3: e00148(15). https://doi.org/10.1128/genomeA.00148-15
  9. Patwari P, Kurz B, Sandy JD, Grodzinsky AJ. 2000. Mannosamine inhibits aggrecanase-mediated changes in the physical properties and biochemical composition of articular cartilage. Arch. Biochem. Biophys. 374: 79−85. https://doi.org/10.1006/abbi.1999.1538
  10. Plass AH, Wong-Palms S, Roughley PJ, Midura RJ, Hascall VC. 1997. Chemical and immunological assay of the nonreducing terminal residues of chondroitin sulfate from human aggrecan. J. Biol. Chem. 272: 20603−20610. https://doi.org/10.1074/jbc.272.33.20603
  11. Plass AH, West LA, Wong-palms S, Nelson FR. 1998. Glycosaminoglycan sulfation in human osteoarthritis. Disease-related alterations at the non-reducing termini of chondroitin and dermatan sulfate. J. Biol. Chem. 273: 12642−12649. https://doi.org/10.1074/jbc.273.20.12642
  12. Prabhakar V, Capila I, Soundararajan V, Raman R, Sasisekharan R. 2009. Recombinant expression, purification, and biochemical characterization of chondroitinase ABC II from Proteus vulgaris. J. Biol. Chem. 284: 974−982. https://doi.org/10.1074/jbc.M806630200
  13. Shaya D, Hahn BS, Park NY, Sim JS, Kim YS, Cygler M. 2008. Characterization of chondroitin sulfate lyase ABC from Bacteroides thetaiotaomicron WAL2926. Biochem. J. 47: 6550−6661.
  14. Shim KW, Kim DH. 2007. Cloning and expression of chondroitinase AC from Bacteroides stercoris HJ-15. Protein. Expr. Purif. 58: 222−228.
  15. Smith-Thomas LC, Stevens J, Fok-Seang J, Faissner A, Rogers JH, Fawcett JW. 1995. Increased axon regeneration in astrocytes grown in the presence of proteoglycan synthesis inhibitors. J. Cell. Sci. 108: 1307−1315.
  16. Voldavsky I, Fuks Z, Isai-Michaeli R, Bashkin P, Levi E, Korner G, et al. 1991. Extracellular matrix resident basic fibroblast growth factor: Implication for the control of angiogenesis. J. Cell. Biochem. 45: 167−176. https://doi.org/10.1002/jcb.240450208