Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Biological activities of Brassica rapa (Turnip) callus extracts by plant cell culture technology

식물세포배양기술을 이용한 순무 캘러스 추출물의 생리활성

  • Shin, Su Young (Division of Ecological Conservation, National Institute of Ecology (NIE)) ;
  • Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Hwang, You Jin (Department of Biomedical Engineering, College of Health Science, Gachon University)
  • 신수영 (국립생태원 생태보전연구실) ;
  • 모상현 ((주)바이오에프디엔씨) ;
  • 황유진 (가천대학교 의용생체공학과)
  • Received : 2016.03.04
  • Accepted : 2016.04.30
  • Published : 2016.06.30
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Abstract

The purpose of this study was to investigate biological activities of Brassica rapa (Turnip) plant callus extracts of Ganghwa-gun of Incheon city using water, ultrasonic wave and ethanol extractions to develop functional materials. DPPH radical scavenging activities of the callus extracts were increased in a concentration-dependent manner, as compared with control. The astringent effects of the ethanol extracts were higher, as compared to water and ultrasonic extracts. In the collagen synthesis assay, the ethanol extract showed significant anti-wrinkle effects of 59% and 78% at a concentration of 5 ppm and 10 ppm, respectively. These results suggested that water, ultrasonic wave and ethanol extracts of turnip plant calluses are natural antioxidant sources. Especially, the ethanol extract can be regarded as a functional, natural cosmetic material with astringent and anti-wrinkle effects.

본 연구에서는 인천광역시 강화군의 특화작물을 이용한 기능성 소재를 개발하기 위해 순무 캘러스를 배양하고 열수, 초음파 그리고 에탄올 추출하여 생리활성을 평가하였다. 순무 캘러스의 열수, 초음파 그리고 에탄올 추출물에서 DPPH radical소거활성이 대조군에 비해 농도 의존적으로 증가하였다. 순무 캘러스 에탄올 추출물의 수렴효과는 열수 및 초음파 추출물보다 높게 나타났다. 콜라겐 생합성량을 측정한 결과 순무 캘러스 에탄올 추출물의 5 ppm과 10 ppm 농도에서 59%와 78%의 주름개선 효과를 확인하였다. 이러한 결과들은 순무 캘러스의 열수, 초음파 그리고 에탄올 추출물이 천연 항산화 소재로의 활용이 가능할 것으로 사료되며 특히, 에탄올 추출물은 수렴효과와 주름개선 효과가 뛰어나 기능성 화장품 천연소재로서의 활용이 가능할 것으로 기대된다.

Keywords

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