Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Cytotoxic, Anti-Inflammatory and Adipogenic Effects of Inophyllum D, Calanone, Isocordato-oblongic acid, and Morelloflavone on Cell Lines

  • Taher, Muhammad (Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia) ;
  • Aminuddin, Amnani (Department of Biomedical Science, Faculty of Science, International Islamic University Malaysia) ;
  • Susanti, Deny (Department of Chemistry, Faculty of Science, International Islamic University Malaysia) ;
  • Aminudin, Nurul Iman (Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia) ;
  • On, Shamsul (Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia) ;
  • Ahmad, Farediah (Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia) ;
  • Hamidon, Hanisuhana (Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia)
  • Received : 2015.11.23
  • Accepted : 2016.01.24
  • Published : 2016.06.30
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Abstract

This paper reports in vitro cytotoxic, anti-inflammatory and adipocyte diffentiation with adipogenic effects of coumarins inophyllum D (1) and calanone (2), and a chromanone carboxylic acid namely isocordato-oblongic acid (3) isolated from Calophyllum symingtonianum as well as a biflavonoid morelloflavone (4) isolated from Garcinia prainiana on MCF-7 breast adenocarcinoma RAW 264.7 macrophages and 3T3-L1 preadipocytes cells, respectively. The cytotoxicity study on MCF-7 cell was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation, respectively. In the cytotoxicity study, inophyllum D (1) was the only compounds that exhibited significant cytotoxic effect against MCF-7 cell with $IC_{50}$ of $84{\mu}g/mL$. Further, all by inhibiting the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages of nitrite concentration with production. In addition, the compounds also exhibited adipogenic effects on 3T3-L1 pre-adipocytes by stimulating lipid formation. Thus, this study may provide significant input in discovery of the potential effects cytotoxic, anti-inflammatory and adipogenic agents.

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