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First Report of Curvularia Leaf Blight Caused by Curvularia trifolii on Creeping Bentgrass in Korea

Curvularia trifolii에 의한 크리핑벤트그래스 잎마름병 발생

  • 성창현 ((주)한울 기업부설 잔디과학연구소) ;
  • 구준학 ((주)한울 기업부설 잔디과학연구소) ;
  • 김정호 (한국잔디연구소) ;
  • 윤정호 ((주)한울 기업부설 잔디과학연구소) ;
  • 이정한 (한국잔디연구소) ;
  • 심규열 (한국잔디연구소) ;
  • 곽연식 (경상대학교 농업생명과학연구원) ;
  • 장석원 (한국골프대학 골프코스매니지먼트과)
  • Received : 2016.05.09
  • Accepted : 2016.06.08
  • Published : 2016.06.30

Abstract

Curvularia leaf blight of creeping bentgrass (Agrostis stolonifera) putting green by caused Curvularia trifolii was observed in Hapcheon, Korea. In July to September 2014, curvularia leaf blight developed on leaf blades of creeping bentgrass as small water-soaked lesions that subsequently turned into dark-colored, necrotic spots. The spots were expanded and became gray, grayish-brown, or light brown, circular to oblong lesions with purple to dark brown borders that often were surrounded by a yellow halo. The necrotic lesions coalesced, became irregular in shape and caused tip or complete blighting of the leaves. Blighted leaf blades appeared grayish-white to tan. The fungus was identified by morphological characters and 16S rDNA sequencing as C. trifolii. Conidia of the pathogen were short, with predominantly 3-septa, straight or often curved, with end cells frequently paler than intermediate cells. Size of the 3-septate conidia in culture are $26{\sim}28{\times}11{\sim}12{\mu}m$. Pathogenicity of the fungus was proved by artificial inoculation on the host. This is the first report of C. trifolii causing leaf blight on creeping bentgrass in Korea.

2014년 7월부터 9월까지 경상남도 합천 지역의 크리핑벤트그래스(Agrostis stolonifera) 퍼팅 그린에서 Curvularia trifolii에 의한 Curvularia 잎마름병이 관찰되었다. Curvularia 잎마름병은 크리핑벤트그래스 잎몸에서 수침상으로 시작되어 진한 괴사반점으로 변한 다음, 크기가 확대되어 회갈색 또는 밝은 갈색의 동그란 타원형 병반이 되었다. 반점의 둘레는 자주 분홍 또는 진한 갈색의 경계를 갖으며 둥그런 무리(halo)를 보였다. 괴사 반점들은 융합되어 불규칙한 모양이 되었고 잎끝 또는 잎 전체가 말랐다. 마른 잎몸은 회백색이나 황갈색을 나타냈다. 그 균은 균학적 특성과 16S rDNA 서열 분석에 의해 동정되었다. 병원균의 분생포자는 크기가 짧았고, 보통 3개의 격막을 가졌으며, 곧거나 자주 휘었는데 양끝 세포는 일반적으로 가운데 세포보다 투명하였다. 3개의 격막을 지닌 분생포자의 배지상 크기는 $26{\sim}28{\times}11{\sim}12{\mu}m$였다. 기주에 대한 균의 인공접종으로 병원성이 확인되었다. 본 논문은 C. trifolii가 크리핑벤트그래스에 Curvularia 잎마름병을 일으키는 우리나라 최초의 보고이다.

Keywords

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