Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Suppressive Effect of Der p 2 on Constitutive Neutrophil Apoptosis by Cytokine Secretion of Normal and Allergic Lymphocytes

정상인과 알레르기 환자의 림프구에서 Der p 2에 의한 사이토카인 분비가 호중구의 세포고사 억제에 미치는 효과

  • Kim, In Sik (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Lee, Na Rae (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Lee, Ji-Sook (Department of Clinical Laboratory Science, Wonkwang Health Science University)
  • 김인식 (을지대학교 대전캠퍼스 임상병리학과) ;
  • 이나래 (을지대학교 대전캠퍼스 임상병리학과) ;
  • 이지숙 (원광보건대학교 임상병리과)
  • Received : 2016.04.26
  • Accepted : 2016.05.30
  • Published : 2016.06.30
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Abstract

Der p 2 is the major allergen of the house dust mite (HDM) associated with the development of allergic diseases. The pathogenic mechanism of the allergy is related to cytokine release of lymphocytes and constitutive apoptosis of neutrophils. In the present study, we examined whether Der p 2 induces cytokine release of lymphocytes, which is involved in regulation of neutrophil apoptosis. In normal and allergic subjects, Der p 2 enhanced the secretion of IL-6, IL-8, MCP-1, and GM-CSF in a time-dependent manner. Although Der p 2 was weakly effective against neutrophil apoptosis, conditioned media collected from normal and allergic lymphocytes after Der p 2 treatment inhibited the apoptosis of normal and allergic neutrophils. Der p 2 showed stronger inhibition of apoptosis of allergic neutrophils cocultured with allergic lymphocytes than normal neutrophils cocultured with normal lymphocytes. These findings improve our understanding of the role of Der p 2 in regulation of lymphocytes and neutrophils and will enable elucidation of allergy pathogenesis.

Der p 2는 알레르기 질환과 관련이 있는 집먼지 진드기의 주요 알러젠이다. 알레르기 질환의 병인기전은 림프구의 사이토카인 분비와 호중구의 세포고사와 관련이 있다. 본 연구에서는 Der p 2가 림프구의 사이토카인 분비를 유도하고, 유도된 사이토카인이 호중구의 세포고사 조절에 효과가 있는 지를 확인하였다. Der p 2는 정상인과 알레르기 질환의 림프구에서 IL-6, IL-8, MCP-1, GM-CSF의 분비를 증가시켰다. Der p 2는 호중구의 세포고사에 어떠한 효과도 없었지만, Der p 2로 림프구를 자극한 뒤 모은 상층액이 호중구의 자발적 세포고사를 억제시켰다. Der p 2는 정상인의 림프구와 호중구를 공동배양에서 자극한 것 보다 알레르기 질환의 림프구와 호중구의 공동배양에서 자극했을 때 호중구의 세포고사를 더 크게 억제시켰다. Der p 2의 림프구와 호중구의 조절기전 규명은 알레르기 질환의 병인기전을 규명하는데 유용한 결과가 될 것이다.

Keywords

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