생체재료학회지 (Biomaterials Research)

  • 제20권4호
  • /
  • Pages.228-235
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  • 2016
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  • 1226-4601(pISSN)
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  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지
DOI QR코드

DOI QR Code

A branched TAT cell-penetrating peptide as a novel delivery carrier for the efficient gene transfection

  • Jeong, Chanuk (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Yoo, Jisang (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Lee, DaeYong (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Kim, Yeu-Chun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
  • 투고 : 2016.06.21
  • 심사 : 2016.08.18
  • 발행 : 2016.12.01
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Cell penetrating peptides (CPPs) as one class of non-viral vectors, have been widely explored as a delivery tool due to their cell-penetrating capability with low cytotoxicity. However, CPPs have reported to have low gene transfection efficiency mainly due to the fact that DNA is larger than other biomolecules. On the other hand, the conventional linear CPPs are unstable for constructing the DNA complexes with it. Thus, here we designed a branched CPP using disulfide bridges based on the linear TAT peptide, to enhance the gene delivery efficiency in a better way. Results: The branched TAT (BTAT) was synthesized by the DMSO oxidation method and showed high-molecular-weight about 294 kDa. The resulting BTAT was complexed with plasmid green fluorescence protein (pGFP) gene at various N/P ratios. The gene transfection efficiency was assessed on HeLa cells after treating with BTAT/pGFP complexes, showed high gene transfection efficiency as conformed by flowcytometry followed by confocal laser scanning microscopy (CLSM) visualization. Conclusion: The novel BTAT/pGFP complex exhibited significantly higher stability and redox cleavability by reducing agent. In addition, BTAT showed higher transfection efficiency approximately 40-fold than those of the TAT and mTAT complexes. Our primary experiments demonstrated the potential of BTAT as a suitable candidate for gene delivery and it could be applied for various types of gene delivery platforms.

키워드

과제정보

연구 과제 주관 기관 : Advanced Biomass R&D Center (ABC), Ministry of Science, ICT and Future Planning

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