Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of Styela clava Tunic after Alkaline Treatment

알칼리처리에 따른 미더덕 껍질의 이화학적 특성

  • Kim, Min Jung (Department of Food Science and Nutrition, and Kimchi Research Institute) ;
  • Kim, Won Baek (Department of Food Science and Nutrition, and Kimchi Research Institute) ;
  • Hwang, Ji Hoe (Department of Food Science and Nutrition, and Kimchi Research Institute) ;
  • Kim, Suae (Department of Food Science and Nutrition, and Kimchi Research Institute) ;
  • Kim, Bo Ram (Department of Food Science and Nutrition, and Kimchi Research Institute) ;
  • Koo, Kyoung Yoon (Department of Food Science and Nutrition, and Kimchi Research Institute) ;
  • Son, Hong Joo (Department of Life Science and Environment Biochemistry, Pusan National University) ;
  • Hwang, Dae Youn (Department of Biomaterials Science, Pusan National University) ;
  • Jung, Young Jin (Department of Biomaterials Science, Pusan National University) ;
  • Lee, Heeseob (Department of Food Science and Nutrition, and Kimchi Research Institute)
  • 김민정 (부산대학교 식품영양학과 및 김치연구소) ;
  • 김원백 (부산대학교 식품영양학과 및 김치연구소) ;
  • 황지회 (부산대학교 식품영양학과 및 김치연구소) ;
  • 김수애 (부산대학교 식품영양학과 및 김치연구소) ;
  • 김보람 (부산대학교 식품영양학과 및 김치연구소) ;
  • 구경윤 (부산대학교 식품영양학과 및 김치연구소) ;
  • 손홍주 (부산대학교 생명환경화학과) ;
  • 황대연 (부산대학교 바이오소재과학과) ;
  • 정영진 (부산대학교 바이오소재과학과) ;
  • 이희섭 (부산대학교 식품영양학과 및 김치연구소)
  • Received : 2015.12.07
  • Accepted : 2016.04.15
  • Published : 2016.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to evaluate the effect of alkaline treatment on Styela clava tunic (SCT). Considerable damage to the surface of alkali-treated SCT was observed by scanning electron microscopy (SEM) in a concentration-dependent manner upon alkaline treatment. The amount of crystalline region in SCT gradually increased with increasing NaOH concentration, which was analyzed by X-ray diffraction and thermogravimetric analysis. The initial enzymatic reaction of Celluclast toward SCT was elevated by treatment with NaOH up to 1.0 N concentration due to disruption of the SCT surface by promoting binding of enzymes with SCT. However, in the late stage of the enzyme reaction, hydrolysis rate decreased with elevation of NaOH concentration, thereby increasing the amount of non-reacted residuals. This result was due to the increase in the crystalline regions in SCT.

본 연구에서는 미더덕 껍질(SCT)의 전처리과정에 사용되는 수산화나트륨을 농도별로 처리하여 알칼리처리에 따른 SCT 셀룰로오스의 물리화학적 특성 및 효소학적 특성에 대한 연구를 수행하였다. 알칼리처리 한 SCT를 주사전자현미경으로 분석한 결과 알칼리처리에 의해 표면에 농도 의존적으로 상당히 많은 손상을 초래하는 것을 확인할 수 있었으며, X-선 회절 분석을 통한 결정화도의 분석으로 알칼리처리에 따라서 SCT 셀룰로오스의 결정화도가 증가함을 알 수 있었다. Celluclast를 이용한 효소의 초기반응에서는 알칼리처리에 따른 SCT 표면 손상과 효소반응이 밀접한 관계가 있었으나 장시간 동안 완전분해를 하는 경우에는 알칼리처리로 인한 결정화도의 증가로 인해 분해되지 않고 남는 SCT가 많음을 확인할 수 있었다. 따라서 SCT의 전처리 시에 알칼리 사용을 최대한 적게 사용하거나 결정화도를 감소시킬 수 있는 대체 기술에 대한 연구가 필요할 것으로 판단된다.

Keywords

References

  1. Houghton DR, Millar RH. 1960. Spread of the ascidian, Styela mammiculata Carlisle. Nature 185: 862.
  2. Hillock KA, Costello MJ. 2013. Tolerance of the invasive tunicate Styela clava to air exposure. Biofouling 29: 1181-1187. https://doi.org/10.1080/08927014.2013.832221
  3. Wonham MJ, Carlton JT. 2005. Trends in marine biological invasions at local and regional scales: the Northeast Pacific Ocean as a model system. Biol Invasions 7: 369-392. https://doi.org/10.1007/s10530-004-2581-7
  4. Davis MH, Davis ME. 2005. Styela clava (Tunicata: Ascidiacea) - a new addition to the fauna of the Portuguese coast. J Mar Biol Assoc UK 85: 403-405. https://doi.org/10.1017/S002531540501132Xh
  5. Ko SC, Lee JK, Byun HG, Lee SC, Jeon YJ. 2012. Purification and characterization of angiotensin I-converting enzyme inhibitory peptide from enzymatic hydrolysates of Styela clava flesh tissue. Process Biochem 47: 34-40. https://doi.org/10.1016/j.procbio.2011.10.005
  6. Lee KH, Kim MG, Hong BI, Jung BC, Lee DH, Park CS. 1995. Seasonal variations of taste components in warty sea squirt (Styela clava). J Korean Soc Food Nutr 24: 274-279.
  7. Lee KH, Park CS, Hong BI, Jung BC, Cho HS, Jea YG. 1995. Seasonal variations of nutrients in warty sea squirt (Styela clava). J Korean Soc Food Nutr 24: 268-273.
  8. Information System Division. 2014. Statistics for oceans and fisheries. Ministry of Oceans and Fisheries, Sejong, Korea. p 69,74.
  9. Ahn SH, Jung SH, Kang SJ, Jeong TS, Choi BD. 2003. Extraction of glycosaminoglycans from Styela clava tunic. Korean J Biotechnol Bioeng 18: 180-185.
  10. Jung YJ. 2008. Properties of regenerated cellulose films prepared from the tunicate Styela clava. J Kor Fish Soc 41: 237-242.
  11. National Institute of Fisheries Science. 2009. Chemical composition of marine products in Korea 2009. National Institute of Fisheries Science, Busan, Korea. p 88-89.
  12. Seong KY, Lee S, Yim SG, Son HJ, Lee YH, Hwang DY, Jung YJ. 2015. Study for biodegradability of cellulose derived from Styela clava tunics. Text Coloration Finish 27: 149-154. https://doi.org/10.5764/TCF.2015.27.2.149
  13. Choi SY, Choi EY, Lee KE, Song AS, Park SH, Lee SC. 2012. Preparation and quality analysis of fish paste containing Styela clava tunic. J Korean Soc Food Sci Nutr 41: 1591-1595. https://doi.org/10.3746/jkfn.2012.41.11.1591
  14. Kim SK, Kim SW, Noh SJ, Kim YJ, Kang JH, Lee SC. 2013. Qualities of konjac containing tunic extract from Styela clava. J Korean Soc Food Sci Nutr 42: 410-414. https://doi.org/10.3746/jkfn.2013.42.3.410
  15. Seong KY, Koh EK, Lee S, Kwak MH, Son HJ, Lee HS, Hwang DY, Jung YJ. 2015. Preparation and characterization of high absorptive cellulose film derived from Styela Clava tunic for wound dressing. Text Coloration Finish 27: 70-79. https://doi.org/10.5764/TCF.2015.27.1.70
  16. Kim SM, Lee JH, Jo JA, Lee SC, Lee SK. 2005. Development of a bioactive cellulose membrane from sea squirt skin for bone regeneration - a preliminary research. J Korean Assoc Oral Maxillofac Surg 10: 440-453.
  17. Segal L, Creely JJ, Martin AE, Conrad CM. 1959. An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29: 786-794. https://doi.org/10.1177/004051755902901003
  18. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428. https://doi.org/10.1021/ac60147a030
  19. Kim SM, Park JM, Kang TY, Kim YS, Lee SK. 2012. Purification of squirt cellulose membrane from the cystic tunic of Styela clava and identification of its osteoconductive effect. Cellulose 20: 655-673.
  20. Gabhane J, William SP, Vaidya AN, Das S, Wate SR. 2015. Solar assisted alkali pretreatment of garden biomass: Effects on lignocellulose degradation, enzymatic hydrolysis, crystallinity and ultra-structural changes in lignocellulose. Waste Manag 40: 92-99. https://doi.org/10.1016/j.wasman.2015.03.002
  21. Kim TH, Kim JS, Sunwoo C, Lee YY. 2003. Pretreatment of corn stover by aqueous ammonia. Bioresour Technol 90: 39-47. https://doi.org/10.1016/S0960-8524(03)00097-X
  22. Gabhane J, William P, Vaidya AN, Mahapatra K, Chakrabarti T. 2011. Influence of heating source on the efficacy of lignocellulosic pretreatment - a cellulosic ethanol perspective. Biomass Bioenergy 35: 96-102. https://doi.org/10.1016/j.biombioe.2010.08.026
  23. Krässig HA. 1993. Cellulose, structure, accessibility and reactivity. Gordon and Breach Publishers, Philadelphia, PA, USA. p 64-103.
  24. Meng A, Chen S, Zhou H, Long Y, Zhang Y, Li Q. 2015. Pyrolysis and simulation of typical components in wastes with macro-TGA. Fuel 157: 1-8. https://doi.org/10.1016/j.fuel.2015.04.056

Cited by

  1. 오만둥이(Styela plicata)에서 글리코스아미노글리칸의 최적 추출조건 설정 및 주름개선 효능 vol.53, pp.5, 2020, https://doi.org/10.5657/kfas.2020.0717
  2. Physicochemical properties and antioxidant activities of spray-dried powder from safflower extract vol.28, pp.2, 2021, https://doi.org/10.11002/kjfp.2021.28.2.218