Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models

  • Kwon, Han Ol (Department of Medical Nutrition, Kyung Hee University) ;
  • Lee, Minhee (Department of Medical Nutrition, Kyung Hee University) ;
  • Kim, Ok-Kyung (Department of Medical Nutrition, Kyung Hee University) ;
  • Ha, Yejin (Department of Medical Nutrition, Kyung Hee University) ;
  • Jun, Woojin (Division of Food and Nutritional Science, Chonnam National University) ;
  • Lee, Jeongmin (Department of Medical Nutrition, Kyung Hee University)
  • Received : 2015.08.26
  • Accepted : 2016.01.06
  • Published : 2016.06.01
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Abstract

BACKGROUND/OBJECTIVES: The inhibitory effect of Hijikia fusiforme (HF) extracts on degenerative osteoarthritis was examined in primary cultured rat cartilage cells and a monosodium iodoacetate (MIA)-induced osteoarthritis rat model. MATERIALS/METHODS: In vitro, cell survival and the expression of matrix metalloproteinases (MMPs), collagen type I, collagen type II, aggrecan, and tissue inhibitor of metalloproteinases (TIMPs) was measured after $H_2O_2$ ($800{\mu}M$, 2 hr) treatment in primary chondrocytes. In vivo animal study, osteoarthritis was induced by intra-articular injection of MIA into knee joints of rats, and then RH500, HFE250 and HFE500 were administered orally once a day for 28 days. To determine the anti-inflammatory effects of HFE, nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$) expression were measured. In addition, real-time PCR was performed to measure the genetic expression of MMPs, collagen type I, collagen type II, aggrecan, and TIMPs. RESULTS: In the in vitro assay, cell survival after $H_2O_2$ treatment was increased by HFE extract (20% EtOH). In addition, anabolic factors (genetic expression of collagen type I, II, and aggrecan) were increased by HFE extract (20% EtOH). However, the genetic expression of MMP-3 and 7, known as catabolic factors were significantly inhibited by treatment with HFE extract (20% EtOH). In the in vivo assay, anabolic factors (genetic expression of collagen type I, II, aggrecan, and TIMPs) were increased by oral administration of HFE extract. However, the genetic expression of MMP-3 and 7, known as catabolic factors, and production of NO and $PGE_2$ were significantly inhibited by treatment with oral administration of HFE extract. CONCLUSION: HFE extract inhibited articular cartilage degeneration through preventing extracellular matrix degradation and chondrocyte injury.

Keywords

References

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