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Glucosyl Rubusosides by Dextransucrases Improve the Quality of Taste and Sweetness

  • Ko, Jin-A (Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ryu, Young Bae (Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Ji-Young (Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Cha Young (Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Joong Su (Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Nam, Seung-Hee (Department of Food Science and Technology and BK21 Plus Program, Graduate School of Chonnam National University and Bioenergy Research Center) ;
  • Lee, Woo Song (Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Young-Min (Department of Food Science and Technology and BK21 Plus Program, Graduate School of Chonnam National University and Bioenergy Research Center)
  • Received : 2016.01.04
  • Accepted : 2016.01.25
  • Published : 2016.03.28

Abstract

Glucosyl rubusosides were synthesized by two dextransucrases. LcDexT was obtained from Leuconosotoc citreum, that LlDexT was obtained from Leuconostoc lactis. LcDexT and LlDexT regioselectively transferred a glucosyl residue to the 13-O-glucosyl moiety of rubusoside with high yield of 59-66% as analyzed by TLC and HPLC. Evaluation of the sweetness of these glucosyl rubusosides showed that their quality of taste, in particular, was superior to that of rubusoside. These results indicate that transglucosylation at the 13-O-glucosyl moiety of rubusoside by different regioselective dextransucrases can be applicable for increasing its sweetness and quality of taste.

Keywords

References

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