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Differential Chemokine Signature between Human Preadipocytes and Adipocytes

  • Rosa Mistica C. Ignacio (Department of Biochemistry and Cancer Biology, Meharry Medical College) ;
  • Carla R. Gibbs (Department of Biochemistry and Cancer Biology, Meharry Medical College) ;
  • Eun-Sook Lee (Department of Physiology, Meharry Medical College) ;
  • Deok-Soo Son (Department of Biochemistry and Cancer Biology, Meharry Medical College)
  • Received : 2016.02.20
  • Accepted : 2016.05.25
  • Published : 2016.06.30
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Abstract

Obesity is characterized as an accumulation of adipose tissue mass represented by chronic, low-grade inflammation. Obesity-derived inflammation involves chemokines as important regulators contributing to the pathophysiology of obesity-related diseases such as cardiovascular disease, diabetes and some cancers. The obesity-driven chemokine network is poorly understood. Here, we identified the profiles of chemokine signature between human preadipocytes and adipocytes, using PCR arrays and qRT-PCR. Both preadipocytes and adipocytes showed absent or low levels in chemokine receptors in spite of some changes. On the other hand, the chemokine levels of CCL2, CCL7-8, CCL11, CXCL1-3, CXCL6 and CXCL10-11 were dominantly expressed in preadipocytes compared to adipocytes. Interestingly, CXCL14 was the most dominant chemokine expressed in adipocytes compared to preadipocytes. Moreover, there is significantly higher protein level of CXCL14 in conditioned media from adipocytes. In addition, we analyzed the data of the chemokine signatures in adipocytes obtained from healthy lean and obese postmenopausal women based on Gene Expression Omnibus (GEO) dataset. Adipocytes from obese individuals had significantly higher levels in chemokine signature as follows: CCL2, CCL13, CCL18-19, CCL23, CCL26, CXCL1, CXCL3 and CXCL14, as compared to those from lean ones. Also, among the chemokine networks, CXCL14 appeared to be the highest levels in adipocytes from both lean and obese women. Taken together, these results identify CXCL14 as an important chemokine induced during adipogenesis, requiring further research elucidating its potential therapeutic benefits in obesity.

Keywords

Acknowledgement

This research was supported, in whole or in part, by National Institutes of Health as the following grants: NIGMS SC1 089630 and R01ES024756 (E.L.), and NIAID SC1AI089073 and NCI SC1CA200519 (D.S.). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NIH.

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