Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Shrimp (Pandalopsis dispar) waste hydrolysate as a source of novel β-secretase inhibitors

  • Li-Chan, Eunice C.Y. (Food, Nutrition & Health Program, Faculty of Land & Food Systems, The University of British Columbia) ;
  • Cheung, Imelda W.Y. (Food, Nutrition & Health Program, Faculty of Land & Food Systems, The University of British Columbia) ;
  • Byun, Hee-Guk (Department of Marine Biotechnology, Gangneung-Wonju National University)
  • Received : 2016.01.04
  • Accepted : 2016.01.28
  • Published : 2016.04.30
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Abstract

In this study, purified peptides from shrimp waste hydrolysates (SWHs) were examined for their inhibitory effects against ${\beta}$-secretase. During consecutive purification using a Sephadex G-25 column chromatography and high performance liquid chromatography on a C18 column, a potent ${\beta}$-secretase inhibitory peptide Asp-Val-Leu-Phe-His (629 Da) was isolated and identified from SWH24 by Q-TOF MS/MS and the $IC_{50}$ value was determined to be $92.70{\mu}M$. The ${\beta}$-secretase inhibition patterns of the purified peptides were found to be competitive. Among synthesized ${\beta}$-secretase inhibitory peptides, Leu-Phe-His had higher ${\beta}$-secretase inhibitory activity than the others. The result of this study suggests that the ${\beta}$-secretase inhibitory peptide derived from SWH24 could be potential candidates to develop nutraceuticals and pharmaceuticals.

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References

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