DOI QR코드

DOI QR Code

Antigenicity of Purified Bee Venom Gel from Honeybee (Apis mellifera L.) in Guinea Pigs

기니픽에서 정제봉독겔의 광독성 평가

  • Han, Sang Mi (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Hong, In Phyo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Woo, Soon Ok (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kim, Se Gun (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Jang, Hye Ri (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Park, Kyun Kyu (Catholic University of Daegu School of Medicine)
  • 한상미 (국립농업과학원 농업생물부) ;
  • 홍인표 (국립농업과학원 농업생물부) ;
  • 우순옥 (국립농업과학원 농업생물부) ;
  • 김세건 (국립농업과학원 농업생물부) ;
  • 장혜리 (국립농업과학원 농업생물부) ;
  • 박관규 (대구가톨릭대학교 의과대학)
  • Received : 2015.12.14
  • Accepted : 2016.02.25
  • Published : 2016.04.30

Abstract

This study was performed to examine the skin phototoxicity of purified bee venom (Apis mellifera L.) collected using bee venom collector. To confirm whether the gel containing purified bee venom (BV gel) causes photototoxicity when used for the skin medicinal products, phototoxicity testing was conducted using guinea pig models. The BV gel (0.1 ml/site) was administered transdermally to guinea pigs. 8-MOP was used to introduce positive control response. After administration, the guinea pigs were irradiated with UVA ($15J/cm^2$) with doses based on standard phototoxicity study guidelines. In the weight measurement and clinical observation, BV gel groups didn't show any significant changes compared with control group. BV gel groups did not show any symptoms such as erythema and edema formation of skin. This study demonstrated that BV gel has promising potential external treatment for topical uses that do not induce significant levels of skin phototoxicity.

Keywords

References

  1. 김민정, 박상동, 이아람, 김경호, 장준혁, 김갑성 : 쥐의 Collagen 유발 관절염의 활액에서 단백분해효소의 활성 및 유리기 손상에 미치는 봉독약침의 억제효과. 대한침구학회지 19, 161 (2002).
  2. Kim, H. W., Kwon, Y. B., Ham, T. W., Roh, D. H., Yoon, S. Y., Lee, H. J., Han, H. J., Yang, I. S., Beitz, A. J. and Lee, J. J. : Acupoint stimulation using bee venom attenuates formalininduced pain behavior and spinal cord fos expression in rats. J. Vet. Med. Sci. 65, 349 (2003). https://doi.org/10.1292/jvms.65.349
  3. Piek, T. : Venoms of the Hymenoptera. London, Academic Press, p. 330 (1986).
  4. Habermann, E. and Reiz, K. G. : On the biochemistry of bee venom pep-tides, melittin and apamin. Biochemistry 343, 192 (1965).
  5. Fennell, J. F., Shipman, W. H. and Cole, L. J. : Antibacterial action of a bee venom fraction (melittin) against a penicillinresistant Staphylococcus and other microorganisms. Res. Dev. Tech. Rep. 5, 1 (1967).
  6. Curcio-Vonlanthen, V., Schneider, C. H., Frutig, K., Blaser, K. H. and Kalbacher, H. : Molecular parameters in melittin immunogenicity. J. Pept. Sci. 3, 267 (1997). https://doi.org/10.1002/(SICI)1099-1387(199707)3:4<267::AID-PSC106>3.0.CO;2-7
  7. Rudenko, S. V. and Nipot, E. E. : Modulation of melittininduced hemolysis of erythocytes. Biokhimiia. 61, 2116 (1996).
  8. 한상미, 이광길, 여주홍, 우순옥, 권해용 : 봉독의 간이 정제 방법, 대한민국특허 10-075881 (2007).
  9. 한상미, 김정민, 이광길, 박관규, 장영채 : 정제봉독의 멜라닌 생성 억제 효과. 약학회지 56, 254 (2012).
  10. Han, S. M., Lee, K. G., Yeo, J. H. and Pak, S. C. : Dermal and ocular irritaion studies of honeybee (Apis mellifera L.) venom. Am. J. Chin. Med. 40, 759 (2012).
  11. Han, S. M., Lee, K. G., Park, K. K. and Pak, S. C. : Skin sensitization study of bee venom (Apis mellifera L.) in guinea pigs and rats. Cutan. Ocul. Toxicol. 32, 27 (2013). https://doi.org/10.3109/15569527.2012.698402
  12. Han, S. M., Hong, I. P., Woo, S. O., Chun, S. N., Park, K. K., Nicholls, Y. M. and Pak, S. C. : The beneficial effects of honeybee-venom serum on facial wrinkles in humans. Clin. Interv. Aging 10, 1587 (2015)
  13. Han, S. M., Yeo, J. Y., Baek, H. J., Lin, S. M., Meyer, S. and Molan, P. : Postantibiotic effect of purified melittin from honeybee (Apis mellifera) venom against Escherichia coli and Staphylococcus aureus. J. Asian Nat. Prod. Res. 11, 796 (2009) https://doi.org/10.1080/10286020903164277
  14. Kim, J. Y., Lee, W. R., Kim, K. H., An, H. J., Chang, Y. C., Han, S. M., Park, Y. Y., Pak, S. C. and Park, K. K. : Effects of bee venom against Propionibacterium acnes-induced inflammation in human keratinocytes and monocytes. Int. J. Mol. Med. 35, 1651 (2015). https://doi.org/10.3892/ijmm.2015.2180
  15. 조병군, 권기록 : 기니픽을 이용한 Sweet Bee Venom의 항원성 평가. J. Pharmacopunct. 14, 23 (2011). https://doi.org/10.3831/KPI.2011.14.4.023
  16. 한상미, 홍인표, 우순옥, 김세건, 장혜리, 박관규, 장영채 : 정제 봉독의 아나필락시스 쇼크 반응 연구. 생약학회지 46, 203 (2015).
  17. Lovell, W. W. and Sanders, D. J. : Phototoxicity testing in guinea pigs. Fd. Chem. Toxic. 30, 155 (1992). https://doi.org/10.1016/0278-6915(92)90151-A
  18. Okaumura, Y., Yamacuchi, H., Takyma, S., Kato, H. and Kokubu, M. : Phototoxicity study of a ketoprofen poultice in guinea pigs. J. Toxicol. Sci. 30, 19 (2005). https://doi.org/10.2131/jts.30.19
  19. Draize, J. H. : Dermal toxicity. Appraisal of the safety of chemicals in foods, drugs, and cosmetics, The Association of Food and Drug Officials of the United States, Austin, Texas State Department of Health, p. 46 (1959).

Cited by

  1. 세균을 이용한 정제봉독의 복귀돌연변이시험 vol.32, pp.3, 2016, https://doi.org/10.13103/jfhs.2017.32.3.228