Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Luteinising Hormone Releasing Hormone Analogue and Human Chorionic Gonadotropin on Spermiation in the Tongue Sole, Cynoglossus semilaevis

Luteinising Hormone Releasing Hormone Analogue과 Human Chorionic Gonadotropin 처리가 박대(Cynoglossus semilaevis)의 배정에 미치는 영향

  • Received : 2016.03.21
  • Accepted : 2016.04.11
  • Published : 2016.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Experiments were carried out examine short-term effects of human chorionic gonadotropin (hCG) and long-term effects of luteinising hormone releasing hormone analogue (LHRHa) on milt production of the tongue sole Cynoglossus semilaevis. In the first experiment, each fish was implanted with a blank cholesterol pellet (control), 100 and $200{\mu}g$ LHRHa per kg body weight. In the second experiment, fish were injected with either 100, 200, 400 and 800 IU hCG per kg body weight or same volume of marine fish Ringer's solution. In the first experiment, milt volume was increased in male implanted with $200{\mu}g$ LHRHa pellet compared with other groups at day 10. Injection of 400 and 800 IU hCG resulted in an increase in the milt volume at hour 96 after the treatment. Although statistical difference is unable to confirm because of small milt volume, compared with the control group, hormone pellet-treated groups had a reduction in the mean spermatocrit (Sct) and sperm concentration (Sc). The results suggest that the increase in milt volume is at least partially gonadotropin (GtH)-dependent and increased milt volume has a relationship with milt hydration.

Keywords

References

  1. Clearwater, S. J. & Crim, L. W.(1998). Gonadotropin releasing hormone-analogue treatment increases sperm motility, seminal plasma pH and sperm production in yellowtail flounder Pleuronectes ferrugineus, Fish Physiol. BIochem. 19, 349-357. https://doi.org/10.1023/A:1007759620936
  2. Garcia, L. M. B.(1991). Spermiation response of mature rabbitfish, Siganus guttatus bloch, to luteinzing hormone-releasing hormone analogue (LHRHa) injection, Aquaculture 97, 291-300 https://doi.org/10.1016/0044-8486(91)90271-8
  3. Garcia, L. M. B.(1993). Sustained production of milt in rabbitfish, Siganus guttatus Bloch, by weekly injection of luteinzing hormone-releasing hormone analogue (LHRHa), Aquaculture 113, 261-267. https://doi.org/10.1016/0044-8486(93)90479-I
  4. Harmin, S. A. & Crim, L. W.(1993). Influence of gonadotropic hormone re1easing hormone analog (GnRH-A) on plasma sex steroid profiles and milt production in male winter flounder, Pseudopleuronectes americanus (Walbaum), Fish Physiol. Biochem. 10, 399-619. https://doi.org/10.1007/BF00004506
  5. Kang, H. W..Lim, H. K..Kang, D. Y..Han H. S..Do, Y. H. & Park, J. S.(2012). Maturation and spawning of the female Tongue sole, Cynoglossus semilaevis in the West Coast of Korea, Dev. Reprod. 16, 87-93.
  6. King, H. R. & Young, G.(2001). Milt production bynon-spermiating male Atlantic salmon (Salmo salar) after injection of a commercial gonadotropin releasinghormone analog preparation, 17-dihydroxy -4-pregnen-3-one, alone or in combination, Aquaculture 193, 179-195. https://doi.org/10.1016/S0044-8486(00)00472-5
  7. Lee, C. S..Tamaru, C. S..Kelley, C. D. & Banno, J. E.(1986). Induced spawning of milkfish, Chanos chanos, bya single application of LHRH-analogue, Aquaculture 58, 87-98. https://doi.org/10.1016/0044-8486(86)90158-4
  8. Lim, H. K.(2016). Effects of exogenous hormones on ovulation and gonadal steroid plasma levels in starry flounder, Platichthys stellatus, Aquaculture International, DOI 10.1007/s10499-016-9971-6.
  9. Lim, H. K..Han, H. S. & Chang, Y. J.(2002). Effects of gonadotropin-releasing hormone analog on milt production enhancement in starry flounder Platichthys stellatus, Fisheries Science 68, 1197-1204. https://doi.org/10.1046/j.1444-2906.2002.00555.x
  10. Lim, H. K. & Kim, S. Y.(2007). Effect of exogenous hormones on spermiation in the starry flounder Platichthys stellatus, J. Kor. Fish. Soc. 40(6), 374-379.
  11. Lim, H. K..Pankhurst, N. W. & Fitzgibbon, Q. P.(2004). Effects of slow release gonadotropin releasing hormone analog on milt characteristics and plasma levels of gonadal steroids in greenback flounder, Rhomobosolea tapirina, Aquaculture 240, 505-516. https://doi.org/10.1016/j.aquaculture.2004.06.017
  12. Linhart, O. & Billard, R.(1994). Spermiation and spermquality of European catfish (Silurus glanis L.) after implantation of GnRH analogues and injection of carp pituitary extract, J. Appl. Ichthyol. 10, 182-188. https://doi.org/10.1111/j.1439-0426.1994.tb00157.x
  13. Linhart, O..Peter, R. E..Rothbard, S..Zohar, Y. & Kvasnicka, P.(1995). Spermiation of common tench (Tinca tinca L.) stimulated with injection or implantation of GnRH analogues and injection of carp pituitary extract, Aquaculture 129, 119-121.
  14. Mananos, E..Carrillo, M..Sorbera, L. A..Mylonas, C. C..Asturiano, J. F..Bayarri, M. J..Zohar, Y. & Zanuy, S.(2002). Luteinizing hormone and sexual steroid plasma levels after treatment of European sea bass with sustained-release delivery systems for gonadotropin releasing hormone analogue, J. Fish Biol. 60, 328-339. https://doi.org/10.1111/j.1095-8649.2002.tb00283.x
  15. Moon, S. H..Lim, H. K..Kwon, J. Y..Lee, J. K. & Chang, Y. J.(2003). Increased plasma 17-hydroxyprogesterone and milt production in response to gonadotropin-releasing hormone agonist in captive male starry flounder, Platichthys stellatus, Aquaculture 218, 703-716.
  16. Mylonas, C. C..Gissis, A..Magnus, Y. & Zohar, Y.(1997). Hormonal changes in male white bass (Morone chrysops) and evaluation of mi1t quality after treatment with a sustained-release GnRHa-delivery system, Aquaculture 153, 301-313. https://doi.org/10.1016/S0044-8486(97)00021-5
  17. Mylonas, C. C. & Zohar, Y.(2001). Endocrine regulation and artificial induction of oocyte maturation and spermiation in basses of the genus Morone, Aquaculture 202, 205-220. https://doi.org/10.1016/S0044-8486(01)00772-4
  18. Overseas Fishery Cooperation Foundation of Japan (2009). Names and illustrations of fishes from the East china Sea and Yellow Sea, Nihon Shiko Printing Co. LTD, 784.
  19. Pankhurst N. W.(1994). Effects of gonadotropin releasing hormone analogue, human chorionic gonadotropin and gonadal steroids on mi1t volume in the New Zealand snapper, Pagrus auratus (Sparidae), Aquaculture 125, 185-197. https://doi.org/10.1016/0044-8486(94)90295-X
  20. Pankhurst, N. W. & Poortenaar, C. W.(2000). Milt characteristics and plasma levels of gonadal steroids in greenback flounder Rhombosolea tapirina following treatment with exogenous hormones, Mar. Fresh. Behav. Physiol. 33, 141-159. https://doi.org/10.1080/10236240009387087
  21. Shangguan, B. & Crim, L. W.(1999). Seasonal variationsin spenn production and sperm quality in male winter flounder, Pleuronectes americanus: the effects of hypophysectomy, pituitary replacement therapy, and GnRH-A treatment, Mar. Biol. 134, 19-27. https://doi.org/10.1007/s002270050521
  22. Sorbera, L. A..Mylonas, C. C..Zanuy, S..Carrillo, M. & Zohar, Y.(1996). Sustained administration of GnRHa increases milt volume without altering sperm counts in the sea bass, J. Exp. Zool. 276, 361-368. https://doi.org/10.1002/(SICI)1097-010X(19961201)276:5<361::AID-JEZ6>3.0.CO;2-M
  23. Ueda, H..Kambegawa, A. & Nagahama, Y.(1985). Involvement of gonadotropin and steroid hormones in spermiation in the amago salmon, Oncorhynchus rhodurus and goldfish, Carassius auratus, Gen. Comp. Endocrinol. 59, 24-30.
  24. Vermeirssen, E. L. M..Shields, R. J..Mazorra de Quero, C. & Scott, A. P.(2000). Gonadotrophin-realeasing hormone agonist raises plasma concentrations of progestogens and enhances milt fluidity in male Atlantic halibut (Hippoglossus hippoglossus), Fish Physiol. Biochem. 22, 77-87. https://doi.org/10.1023/A:1007820913009
  25. Zohar, Y. & Mylonas, C.(2001). Endocrine manipulation of spawning in cultured fish: from hormones to genes, Aquaculture 197, 99-136. https://doi.org/10.1016/S0044-8486(01)00584-1