Journal of Plant Biotechnology

  • 제43권1호
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  • Pages.132-137
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  • 2016
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Effects of altering medium strength and sucrose concentration on in vitro germination and seedling growth of Cypripedium macranthos Sw.

  • Huh, Yoon Sun (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Lee, Joung Kwan (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Nam, Sang Young (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Hong, Eui Yon (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Paek, Kee Yoeup (Department of Horticultural Science, Chungbuk National University) ;
  • Son, Sung Won (Plant Conservation Division, Korea National Arboretum of the Korea Forest Service)
  • 투고 : 2016.02.11
  • 심사 : 2016.03.15
  • 발행 : 2016.03.31
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초록

The genus Cypripedium, known as lady's slipper orchid, has been considered to have a considerable marketability as a potted and garden plant with beautiful flowers; however, this species is becoming endangered and, in some places extinct, due to habitat destruction and illegal collections. As such, an optimized artificial propagation system is necessary for its conservation and horticultural cultivation. For the establishment of the in vitro proliferation of the endangered Cypripedium macranthos Sw., native to Korea, the effects of medium strength and sucrose concentrations on germination, protocorm formation and seedling growth were investigated through asymbiotic seed culture. The highest germination rate and protocorm formation rate were obtained with a 1/4 MS medium; higher MS medium strengths did not generate the favorable conditions required for germination and protocorm growth. The overall growth characteristics of roots and buds developed from protocorms were best in cultures of 1/4 MS medium. On this medium containing $10g{\cdot}L^{-1}$ sucrose, both the germination rate and protocorm formation rate increased significantly. The general growth properties of seedlings developed on the medium with $10g{\cdot}L^{-1}$ sucrose were best, showing the highest bud formation rate and root number. Our results demonstrate that the 1/4 MS medium, supplemented with $10g{\cdot}L^{-1}$ sucrose, could improve in vitro germination, and facilitate the growth of seedlings developed from the protocorms of C. macranthos Sw.

키워드

참고문헌

  1. Arditti J (1967) Factors affecting the germination of orchid seeds. Bot Rev 33:1-97 https://doi.org/10.1007/BF02858656
  2. Arditti J (2009) Micropropagation of orchids (Ed.). John Wiley and Sons, Inc., New York, USA. pp. 489
  3. Arditti J, Ernst R (1984) Physiology of germinating orchid seeds. In: Arditti J. (ed.). Orchid biology: reviews and perspectives III. Cornell University Press, Ithaca, New York, USA. pp. 176-222
  4. Arditti J, Ernst R (1992) Micropropagation of Orchids. John Wiley and Sons, Inc, New York, USA. pp. 682
  5. Ballard WW (1987) Sterile propagation of Cypripedium reginae from seeds. Amer Orchid Soc Bull 56:935-46
  6. Cribb P (1997) The genus Cypripedium. Timber Press, Portland, USA. pp. 301
  7. Cribb P, Sandison M (1998) A preliminary assessment of the conservation status of species in the wild. Bot J Linnean Soc 126:183-190
  8. Deb CR, Pongener A (2011) Asymbiotic seed germination and in vitro seedling development of Cymbidium aloifolium (L.) Sw.: a multipurpose orchid. J Plant Biochem Biotechnol 20:90-95 https://doi.org/10.1007/s13562-010-0031-4
  9. Deng L, Zhang Y, Wang MM, Zhao SW (2012) Study on non-asymbiotic germination of Cypripedium macranthos seed. Seed 31:31-39
  10. De Pauw MA, Remphery WR (1993) In vitro germination of three Cypripedium species in relation to time of collection, media and cold treatment. Can J Bot 71:879-885 https://doi.org/10.1139/b93-100
  11. Fast G (1982) European terrestrial orchids (Symbiotic and asymbiotic methods). In: Arditti, J. (ed.) Orchid Biology. II. Reviews and perspectives. Orchid seed germination and seedling culture - a manual. Cornell University Press, Ithaca, New York, USA pp. 309-326
  12. Gibson SI (2000) Plant sugar response pathways. Part of a complex regulatory web. Plant Physiol 124:1532-1539 https://doi.org/10.1104/pp.124.4.1532
  13. Gould AR, Evereet NP, Wang TL, Street HE (1981) Studies on the control of cell cycle in cultured plant cells. I. Effect of nutrient limitation and nutrient starvation. Protoplasma 106:1-13 https://doi.org/10.1007/BF02115957
  14. Harvais G (1974) Notes on the biology of some native orchids of Thunder Bay, their endophytes and symbionts. Can J Bot 52:451-460 https://doi.org/10.1139/b74-059
  15. Harvais G (1982) An improved culture medium for growing the orchid Cypripedium reginae axenically. Can J Bot 60:2547-2555 https://doi.org/10.1139/b82-309
  16. Huh YS (2015) Establishment of in vitro proliferation and cultural practice of endangered Cypripedium macranthos Sw. through asmybiotic seed culture. Chungbuk National Unv. Korea. PhD Diss
  17. Johnson TR, Kane ME, Perez HE (2011) Examining the interaction of light, nutrients and carbohydrates on seed germination and early seedling development of Bletia purpurea (Orchidaceae). Plant Growth Regul 63:89-99 https://doi.org/10.1007/s10725-010-9516-3
  18. Knudson L (1946) A new nutrient solution for the germination of orchid seeds. Bot Gaz 73:1-25
  19. Lee JS, Choi BH (2006) Distribution and red data of wild orchids in the Korean Peninsula. Kor J Plant Taxon 36:335-360 https://doi.org/10.11110/kjpt.2006.36.4.335
  20. Malmgren S (1988) Froforokning av Dactylorhiza i stor skala - en kort manual. Svensk Botanisk Tidskrift 82:61-166
  21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  22. Piao RZ, Wang YL, Zhao HY (2011) Preliminary exploration in in vitro seed culture conditions of Cypripedium macrnathos Sw. J Anhui Agri Sci 39:18428-18429, 18445
  23. Rannback L (2007) Propagation, cultivation and breeding of terrestrial temperate orchids, with focus on Cypripedium spp. Dept of Crop Science, SLU, Bachelor project in the Danish-Swedish Horticulture programme 1
  24. Rasmussen HN (1995) Terrestrial Orchids: From Seed to Mycotrophic Plant. Cambridge University Press, New York, USA. pp. 444
  25. Shim SW, Hahn EJ, Paek KY (2003) In vitro and ex vitro growth of grapevine rootstock '5BB' as influenced by number of air exchanges and the presence or absence of sucrose in culture media. Plant Cell Tiss Organ Cult 75:57-62 https://doi.org/10.1023/A:1024695510994
  26. Shohael AM, Chakrabarty D, Ali MB, Yu KW, Hahn EJ, Lee HL, Paek KY (2006) Enhancement of eleutherosides production in embryogenic cultures of Eleutherococcus sessiliflorus in response to sucrose-induced osmotic stress. Process Biochem 41:512-518 https://doi.org/10.1016/j.procbio.2005.09.005
  27. Texeira da Silva JA (2013) Orchids: Advances in tissue culture, genetics, phytochemistry and transgenic biotechnology. Floriculture and Ornamental Biotechnology 7:1-52
  28. Udomdee W, Wen PJ, Lee CY, Chin SW, Chen FC (2014) Effect of sucrose concentration and seed maturity on in vitro germination of Dendrobium nobile hybrids. Plant Growth Regul 72:249-255 https://doi.org/10.1007/s10725-013-9856-x
  29. Van Waes J (1984) In vitro studie van de kiemingsfysiologie van Westeuropese orchideeen. Diss, Rijksuniversiteit Gent, Faculteit van de Landbouwwetenschappen
  30. Van Waes JM, Debergh PC (1986) In vitro germination of some Western European orchids. Physiologia Plantarum 67:253-261 https://doi.org/10.1111/j.1399-3054.1986.tb02452.x
  31. Wu CH, Dewir YS, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. Plant Biol 49:193-199 https://doi.org/10.1007/BF03030532
  32. Wu ZY, Raven PH, Hong DY (2009) Flora of China. (Ed.) Vol. 25 (Orchidaceae). Science Press and St. Louis: Missouri Botanical Garden Press, Beijing, China. pp. 22-33
  33. Yan N, Hu H, Huang JL, Xu K, Wang H, Zhou ZK (2006) Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds. Plant Cell Tiss Org Cult 84:113-117
  34. Zeng S, Zhang Y, Jaime A, Teixeira da Silva JA, Wu K, Zhang J, Duan J (2014) Seed biology and in vitro seed germination of Cypripedium. Crit Rev Biotechnol 34:358-371 https://doi.org/10.3109/07388551.2013.841117
  35. Zha XQ, Luo JP, Jiang ST, Wang JH (2007) Enhancement of polysaccharide production in suspension cultures of protocormlike bodies from Dendrobium huoshanense by optimization of medium compositions and feeding of sucrose. Process Biochem 42:344-351 https://doi.org/10.1016/j.procbio.2006.09.008
  36. Zhang YY, Wu KL, Zhang JX, Deng RF, Duan J, Da Silva JAT, Huang WC, Zeng SJ (2015) Embryo development in association with asymbiotic seed germination in vitro of Paphiopedilum armeniacum S.C. Chen et F.Y. Liu. Sci Rep 5:16356;doi:10.1038/srep16356

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