Journal of Plant Biotechnology

  • 제43권1호
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  • Pages.66-75
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  • 2016
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Efficiency of transformation mediated by Agrobacterium tumefaciens using vacuum infiltration in rice (Oryza sativa L.)

  • Safitri, Fika Ayu (Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University) ;
  • Ubaidillah, Mohammad (Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University) ;
  • Kim, Kyung-Min (Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University)
  • 투고 : 2016.01.25
  • 심사 : 2016.02.15
  • 발행 : 2016.03.31
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초록

Agrobacterium-mediated gene transfer has recently been developed to improve rice transformation. In this study, 3 different transformation methods were tested including soaking, co-cultivation, and vacuum infiltration. Agrobacterium tumefaciens GV3101 harboring the binary vector pGreen:: LeGSNOR was used in this experiment. This study aimed to identify the most appropriate method for transferring LeGSNOR into rice. Vacuum infiltration of the embryonic calli for 5 min in Ilpum resulted in high transformation efficiency based on confirmation by PCR, RT-PCR, and qRT-PCR analyses. In conclusion, we described the development of an efficient transformation protocol for the stable integration of foreign genes into rice; furthermore, the study results confirmed that PCR is suitable for efficient detection of the integrated gene. The vacuum infiltration system is a potentially useful tool for future studies focusing on transferring important genes into rice seed calli, and may help reduce time and effort.

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피인용 문헌

  1. Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus vol.18, pp.1, 2018, https://doi.org/10.1186/s12896-018-0459-5