한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제31권1호
  • /
  • Pages.33-38
  • /
  • 2016
  • /
  • 2508-755X(pISSN)
  • /
  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지
DOI QR코드

DOI QR Code

Isoproterenol과 Melatonin의 첨가가 돼지 단위발생란의 체외발달에 미치는 영향

Effects of Isoproterenol and Melatonin Supplementation on In Vitro Development of Parthenogenetic Activated Oocytes in Pig

  • 정다운 (경남과학기술대학교 동물생명과학과) ;
  • 윤윤진 (경남과학기술대학교 동물생명과학과) ;
  • 박희성 (경남과학기술대학교 동물생명과학과)
  • Jeong, Da-Un (Dept. of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Yun, Yun-Jin (Dept. of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Park, Hee-Sung (Dept. of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology)
  • 투고 : 2016.02.15
  • 심사 : 2016.03.01
  • 발행 : 2016.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, to improve the in vitro development of various cells including cloned embryos, the effects that isoproterenol and melatonin have on in vitro development of porcine parthenogenetic oocytes were investigated. Parthenogenetic activation was induced with electrical stimulation, BSA and 6-DMAP treatment. $10^{-7}M$ of melatonin and isoproterenol ($10^{-10}$, $10^{-12}$ and $10^{-14}M$) were supplemented for in vitro maturation (IVM) and in vitro culture (IVC) medium, with different concentrations. When isoproterenol and melatonin were supplemented in IVM medium with different concentrations, there was no significant (P<0.05) difference of maturation rate in the treatment groups as well as in that of only melatonin. As isoproterenol and melatonin were supplemented in IVM medium with different concentrations, blastocyst rates of isoproterenol $10^{-12}M$ treatment group (37.1%) were significantly (P<0.05) higher than control group (26.0%). Isoproterenol and melatonin were supplemented in IVC medium with different concentrations, then the cleavage rate of $10^{-12}M$ isoproterenol treatment group (82.2%) was significantly (P<0.05) higher than the group that melatonin was only supplemented (70.9%). There was no difference of blastocyst rate between the treatment groups. When isoproterenol and melatonin were supplemented for IVM+IVC medium with different concentrations, the cleavage rate of $10^{-12}M$ isoproterenol treatment group (92.5%) was significantly (P<0.05) higher than the control group (82.8%) and the group that melatonin was only treated (81.6%). The blastocyst rate of $10^{-12}M$ as 45.6% was significantly (P<0.05) higher than control group (25.2%) and melatonin treatment group (31.2%). The cell number of blastocyst in $10^{-12}M$ isoproterenol treatment group $35.5{\pm}3.4$ was significantly (P<0.05) highest. The results of this study showed that the development rate of IVC when both isoproterenol and melatonin were supplemented was higher than when melatonin was only supplemented. Therefore, it is concluded that isoproterenol is rather effective in the activation of melatonin. $10^{-7}M$ melatonin and $10^{-12}M$ isoproterenol were considered suitable concentration.

키워드

참고문헌

  1. Cikos S, Vesela J, Il'kova G, Rehak P, Czikkova S and Koppel J. 2005. Expression of beta adrenergic receptors in mouse oocytes and preimplantation embryos. Molecular Reproduction and Development 71:145-153. https://doi.org/10.1002/mrd.20256
  2. Farahavar A, Shahne AZ, Kohram H and Vahedi V. 2010. Effect of melatonin on in vitro maturation of bovine oocytes. African J. Biotechnology 9:2579-2583.
  3. Garcia JJ, Lopez-Pingarron L, Almeida-Souza P, Tres A, Escudero P, Garcia-Gil FA, Tan DX, Reiter RJ, Ramirez JM and Bernal-Perez M. 2014. Protective effects of melatonin in reducing oxidative stress and in preserving the fluidity of biological membranes: A review. Rev. J. Pineal Research 56:225-237. https://doi.org/10.1111/jpi.12128
  4. Goud AP, Goud PT, Diamond MP, Gonik B and Abu-Soud HM. 2008. Reactive oxygen species and oocyte aging: Role of superoxide, hydrogen peroxide, and hypochlorous acid. Free Radical Biology and Medicine 44:1295-1304. https://doi.org/10.1016/j.freeradbiomed.2007.11.014
  5. Gupta BB, Spessert R and Vollrath L. 2005. Molecular components and mechanism of adrenergic signal transduction in mammalian pineal gland: regulation of melatonin synthesis. Rev. Indian J. Experimental Biology 43:115-149.
  6. Kennaway DJ and Rowe SA. 2000. Effect of stimulation of endogenous melatonin secretion during constant light exposure on 6 sulphatoxymelatonin rhythmicity in rats. J. Pineal Research 28:16-25. https://doi.org/10.1034/j.1600-079x.2000.280103.x
  7. Lord T and Aitken RJ. 2013. Oxidative stress and ageing of the post-ovulatory oocyte. Rev. Reproduction 146:217-227. https://doi.org/10.1530/REP-13-0111
  8. Manjunatha BM, Devaraj M, Gupta PSP, Ravindra JP and Nandi S. 2009. Effect of taurine and melatonin in the culture medium on buffalo in vitro embryo development. Reproduction in Domestic Animals 44:12-16. https://doi.org/10.1111/j.1439-0531.2007.00982.x
  9. Nakano M, Kato Y and Tsunoda Y. 2012. Effect of melatonin treatment on the developmental potential of parthenogenetic and somatic cell nuclear-transferred porcine oocytes in vitro. Zygote 20:199-207. https://doi.org/10.1017/S0967199411000190
  10. Pang YW, An L, Wang P, Yu Y, Yin QD, Wang XH, Xin-Zhang, Yang ML, Min-Guo, Wu Z H and Tian JH. 2013. Treatment of porcine donor cells and reconstructed embryos with the antioxidant melatonin enhances cloning efficiency. J. Pineal Research 54:389-397. https://doi.org/10.1111/jpi.12024
  11. Reiter RJ, Tan DX, Manchester LC, Paredes SD, Mayo JC and Sainz RM. 2009. Melatonin and reproduction revisited. Rev. Biology of Reproduction 81:445-456.
  12. Shi JM, Tian XZ, Zhou GB, Wang L, Gao C, Zhu SE, Zeng SM, Tian JH and Liu GS. 2009. Melatonin exists in porcine follicular fluid and improves in vitro maturation and parthenogenetic development of porcine oocytes. J. Pineal Research 47:318-323. https://doi.org/10.1111/j.1600-079X.2009.00717.x
  13. Takada L, Martins-Junior A, Mingoti GZ, Balieiro JCC and Coelho LA. 2010. Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos. Scientia Agricola 67:393-398. https://doi.org/10.1590/S0103-90162010000400003
  14. Tamura H, Takasaki A, Taketani T, Tanabe M, Kizuka F, Lee L, Tamura I, Maekawa R, Aasada H, Yamagata Y and Sugino N. 2012. The role of melatonin as an antioxidant in the follicle. Rev. J. Ovarian Research 5:5. https://doi.org/10.1186/1757-2215-5-5
  15. Tengattini S, Reiter RJ, Tan DX, Terron MP, Rodella LF and Rezzani R. 2008. Cardiovascular diseases: Protective effects of melatonin. Rev. J. Pineal Research 44:16-25.
  16. 정다운, 윤윤진, 안한솔, 박희성. 2014. Melatonin의 첨가가 돼지 단위발생란의 체외 발달에 미치는 영향. 제 14회 발생 공학 국제심포지엄. Abst. pp111.
  17. 정다운, 윤윤진, 안한솔, 박희성. 2015a. 난포란의 등급에 따른 Melatonin의 첨가가 돼지 단위발생란의 체외 발달에 미치는 영향. 제 15회 발생공학 국제심포지엄. Abst. pp52.
  18. 정다운, 윤윤진, 안한솔, 박희성. 2015b. Isoproterenol의 첨가가 돼지 단위발생란의 체외 발달에 미치는 영향. Reproductive and Developmental Biology. Abst. 39:115.