생약학회지 (Korean Journal of Pharmacognosy)

  • 제47권1호
  • /
  • Pages.73-78
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  • 2016
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

초음파 처리에 의한 인삼꽃대 엑스의 진세노사이드 성분 변화

Changes in Ginsenosides Composition of Ginseng Flower Buds Extracts after an Ultrasonication Process

  • 남윤민 (세명대학교 한방식품영양학부) ;
  • 권주희 (세명대학교 한방식품영양학부) ;
  • 홍정태 (세명대학교 한방식품영양학부) ;
  • 양병욱 (고연 인삼연구소) ;
  • 고성권 (세명대학교 한방식품영양학부)
  • Nam, Yun Min (The Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Kwon, Jue Hee (The Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Hong, Jeong Tae (The Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Yang, Byung Wook (Ginseng Research Center) ;
  • Ko, Sung Kwon (The Department of Oriental Medical Food & Nutrition, Semyung University)
  • 투고 : 2015.12.31
  • 심사 : 2016.02.22
  • 발행 : 2016.03.31
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초록

The purpose of this study is to develop a new preparation process of ginseng (Panax ginseng) flower buds extracts featuring high concentration of ginsenosides Rg2, Rg3, Rg5, F4 and Rh1, red ginseng special components. Chemical transformation from ginseng saponin glycosides to prosapogenin was analyzed by the HPLC. Extracts of ginseng flower buds were processed under several treatment conditions of ultrasonication (at $100^{\circ}C$). The results showed that the quantity of ginsenoside Rg6 increased by over 8.8% at the 16 hours of ultrasonication. Ginseng flower buds ethanol extract compared with other process times. The result of UGF-16 indicates that the ultrasonication processed ginseng flower buds extracts (at $100^{\circ}C$) treated for 16 hours produced the highest amount of ginsenoside F4 (8.833%), Rg3 (2.230%), Rg5 (2.339%) and Rg2 (1.002%).

키워드

참고문헌

  1. Ko, S. K. and Im, B. O. (2009) The science of Korean ginseng, 47-52, Yakupsinmunsa, Seoul.
  2. Yokozawa, T., Kobayashi, T., Oura, H. and Kawashima, Y. (1985) Studies on the mechanism of the hypoglycemic activity of ginsenoside-Rb2 in streptozotocin-diabetic rats. Chem. Pharm. Bull. 33: 869-872. https://doi.org/10.1248/cpb.33.869
  3. Park, J. D. (1996) Recent studies on the chemical constituents of Korean ginseng. Korean J. Ginseng Sci. 20: 389-415.
  4. Ko, S. K., Cho, O. S., Bae, H. M., Im, B. O., Lee, O. H. and Lee, B. Y. (2011) Quantitative analysis of ginsenosides composition in flower buds of various ginseng. J. Appl. Biol. Chem. 54: 154-157.
  5. Tung, N. H., Song, G. Y., Nhiem, N. X., Ding, Y., Tai, B. H., Jin, L. G., Lim, C. M., Hyun, J. W., Park, C. J., Kang, H. K. and Kim, Y. H. (2010) Dammarane-type saponins from the flower buds of Panax ginseng and their intracellular radical scavenging capacity. J. Agric. Food Chem. 58: 868-874. https://doi.org/10.1021/jf903334g
  6. Nguyen, H. T., Song, G. Y., Kim, J. A., Hyun, J. H., Kang, H. K. and Kim, Y. H. (2010) Dammarane-type saponins from the flower buds of Panax ginseng and their effects on human leukemia cells. Bioorg. Med. Chem. Lett. 20: 309-314. https://doi.org/10.1016/j.bmcl.2009.10.110
  7. Yoshikawa, M. 1., Sugimoto, S., Nakamura, S., Sakumae, H. and Matsuda, H. (2007) Medicinal flowers. XVI. New dammarane- type triterpene tetraglycosides and gastroprotective principles from flower buds of Panax ginseng. Chem. Pharm. Bull. 55: 1034-1038. https://doi.org/10.1248/cpb.55.1034
  8. Gwak, H. H., Hong, J. T., Ahn, C. H., Kim, K. J., Kim, S. G., Yoon, S. S., Im, B. O., Cho, S. H., Nam, Y. M. and Ko, S. K. (2015) The change of ginsenoside composition in the ginseng (Panax ginseng) flower buds by the ultrasonication and vinegar process, Nat. Prod. Sci. 21: 1-5.
  9. Jo, H. K., Cho, S, H. and Ko, S. K. (2014) The change of ginsenoside composition in Notoginseng root(Panax notoginseng) extract by the microwave and vinegar process. Kor. J. Pharmacogn. 45: 320-325.
  10. Zheng, H., Jeong, Y., Song, J. and Ji, G. E. (2011) Oral administration of ginsenoside Rh1 inhibits the development of atopic dermatitis-like skin lesions induced by oxazolone in hairless mice. Int. Immunopharmacol. 11: 511-518. https://doi.org/10.1016/j.intimp.2010.12.022
  11. Keum, Y. S., Han, S. S., Chun, K. S., Park, K. K., Park, J. H., Lee, S. K. and Surh, Y. J. (2003) Inhibitory effects of the ginsenoside Rg3 on phorbol ester-induced cyclooxygenase-2 expression, NF-kappaB activation and tumor promotion. Mutat. Res. 523: 75-85.
  12. Kim, N. D., Kang, S. Y., Park, J. H. and Schini-Kerth, V. B. (1999) Ginsenoside Rg3 mediates endothelium-dependent relaxation in response to ginsenosides in rat aorta: role of K+ channels. Eur. J. Pharmacol. 367: 41-49. https://doi.org/10.1016/S0014-2999(98)00898-X
  13. Yang, L., Hao, J., Zhang, J., Xia, W., Dong, X., Hu, X., Kong, F. and Cui, X. (2009) Ginsenoside Rg3 promotes beta-amyloid peptide degradation by enhancing gene expression of neprilysin. J. Pharm. Pharmacol. 61: 375-380. https://doi.org/10.1211/jpp.61.03.0013
  14. Lee, W. M., Kim, S. D., Park, M. H., Cho, J. Y., Park, H. J., Seo, G. S. and Rhee, M. H. (2008) Inhibitory mechanisms of dihydroginsenoside Rg3 in platelet aggregation: critical roles of ERK2 and cAMP. J. Pharm. Pharmacol. 60: 1531-1536. https://doi.org/10.1211/jpp.60.11.0015
  15. Keum, Y. S., Park, K. K., Lee, J. M., Chun, K. S., Park, J. H., Lee, S. K., Kwon, H. and Surh, Y. J. (2000) Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Lett. 150: 41-48. https://doi.org/10.1016/S0304-3835(99)00369-9
  16. Jeong, S. J., Han, S. H., Kim, D. Y., Lee, J. C., Kim, H. S., Kim, B. H., Lee, J. S., Hwang, E. H. and Park, J. K. (2007) Effects of m Rg2, a mixture of ginsenosides containing 60% Rg2, on the ultraviolet B-induced DNA repair synthesis and apoptosis in NIH3T3 cells. Int. J. Toxicol. 26: 151-158. https://doi.org/10.1080/10915810701226370