대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제42권1호
  • /
  • Pages.57-64
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  • 2016
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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두날리엘라 살리나 추출물의 피부 열노화 억제 효과

Inhibitory Effects of Dunaliella salina Extracts on Thermally-Induced Skin Aging

  • 주지혜 ((주)그린솔루션스 기업부설연구소) ;
  • 석지현 ((주)래디안 기업부설연구소) ;
  • 홍인기 ((주)래디안 기업부설연구소) ;
  • 김남경 ((주)그린솔루션스 기업부설연구소) ;
  • 최은미 ((주)그린솔루션스 기업부설연구소)
  • 투고 : 2015.12.17
  • 심사 : 2016.02.24
  • 발행 : 2016.03.30
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초록

자외선과 유사하게 열에 의한 콜라겐 분해와 비정상적인 탄력섬유의 축적을 증가시키는 현상을 열노화라 칭한다. 두날리엘라 살리나는 녹조류로 베타카로틴을 많이 함유하고 있어 건강식품으로 많이 이용되고 있으나 열에 의해 유도된 피부노화에서의 효능은 알려진 바 없다. 본 연구에서는 두날리엘라 살리나 에탄올 추출물의 항-열노화 효능을 확인하였다. 열을 가한 피부섬유아세포를 이용하여 MMP-1과 type I procollagen 발현을 ELISA를 이용해 확인하였다. 두날리엘라 살리나 추출물이 열에 의해 증가된 MMP-1 단백질 발현량을 감소시키며, type I procollagen 단백질 발현량은 증가시킨다는 사실을 확인하였다. 추가적으로, 두날리엘라 살리나 추출물에 의해 콜라겐 합성 과정에 관여하는 것으로 알려진 HSP47 mRNA의 발현이 증가함을 확인하였다. 또한, 두날리엘라 살리나 추출물이 염증매개인자(TGF-${\beta}$, IL-12 등)의 발현을 감소시킴을 확인하였다. 다음으로 두날리엘라 살리나 추출물이 탄력섬유의 구성성분인 tropoelastin과 fibrillin-1 단백질 발현과 MMP-12 발현 조절을 통해 열에 의해 유도된 일광탄력섬유증을 조절하는 효능을 확인하였다. 이 결과를 통해 두날리엘라 살리나 추출물이 열에 의해 유도된 피부열노화를 효과적으로 예방함을 확인하였다.

Just like UV radiation, heat increases collagen degradation and accumulation of abnormal elastin fiber and this is termed thermal skin aging. Dunaliella salina (DS), a green alga, is known for its beta-carotene accumulation, having various applications in the health and nutritional products. However, the effects of DS on heat-induced skin aging remain unexplored. In this study, we performed anti-thermal aging tests of the ethanol extract of DS (DSE). We measured the cellular levels of type I procollagen and MMP-1 using ELISA in human dermal fibroblast cells after heat shock. DSE reduced the expression of MMP-1 protein and increased the expression of type I procollagen. In addition, DSE upregulated the mRNA expression of HSP47 reduced by heat shock, which is involved in collagen synthesis. Also, DSE reduced the expression of inflammation mediator (TGF-${\beta}$, IL-12, etc). We demonstrate that DSE regulates the heat-induced solar elastosis through the regulation of tropoelastin and fibrillin-1, two major proteins of elastic fibers, and MMP-12 expression. These results suggest that DSE may be effective for preventing thermally induced skin aging.

키워드

참고문헌

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피인용 문헌

  1. Ehrh.) in Human Dermal Fibroblasts vol.2018, pp.1741-4288, 2018, https://doi.org/10.1155/2018/4595982