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식물세포배양으로부터 파클리탁셀 회수를 위한 초음파를 이용한 액-액 추출

Ultrasound-Assisted Liquid-Liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures

  • 하건수 (공주대학교 화학공학부) ;
  • 김진현 (공주대학교 화학공학부)
  • Ha, Geon-Soo (Department of Chemical Engineering, Kongju National University) ;
  • Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
  • 투고 : 2015.09.18
  • 심사 : 2015.11.10
  • 발행 : 2016.04.01

초록

본 연구에서는 식물세포배양액으로부터 파클리탁셀을 효율적으로 회수하기 위하여 초음파를 이용한 액-액 추출 공정을 개발하였다. 액-액 추출을 위한 최적의 초음파 파워와 조업 시간은 주어진 하층(메틸렌 클로라이드 층)/상층(메탄올 농축액 층) 비(25%, v/v)에서 각각 250W와 15 min임을 알 수 있었다. 최적 조건 하에서 초음파를 이용한 액-액 추출 공정의 경우 단 1 회 추출로 대부분의 파클리탁셀을 하층(메틸렌 클로라이드 층)으로부터 회수(~92%) 가능하였다. 또한 무기염 첨가에 의한 초음파 상승효과로 인하여 액-액 추출을 위하여 적절한 무기염 농도와 초음파 파워가 요구됨을 알 수 있었다.

In this study, an efficient ultrasound-assisted liquid-liquid extraction process was developed for recovering of paclitaxel from plant cell cultures. The optimal ultrasonic power and operating time were 250 W and 15 min at fixed ratio of bottom phase, methylene chloride to top phase, MeOH (25%, v/v). Under the optimal conditions developed in the present method, most of the paclitaxel (~92%) was recovered from crude extract by a single extraction step. Due to the synergistic effect of ultrasound by the addition of inorganic salt, an appropriate inorganic salt concentration and the ultrasonic power were found to be required for the effective recovery of paclitaxel using ultrasound-assisted liquid-liquid extraction.

키워드

참고문헌

  1. Kim, J. H., "Paclitaxel : Recovery and Purification in Commercialization Step," Korean J. Biotechnol. Bioeng., 21, 1-10(2006).
  2. Kim, G. J. and Kim, J. H., "Enhancement of Extraction Efficiency of Paclitaxel From Biomass Using Ionic Liquid-methanol Cosolvents Under Acidic Conditions," Process Biochem., 50, 989- 996(2015). https://doi.org/10.1016/j.procbio.2015.03.009
  3. Hsiao, J. R., Leu, S. F. and Huang, B. M., "Apoptotic Mechanism of Paclitaxel-induced Cell Death in Human Head and Neck Tumor Cell Lines," J. Oral Pathol. Med., 38, 188-197(2009). https://doi.org/10.1111/j.1600-0714.2008.00732.x
  4. Rao, K., Hanuman, J., Alvarez, C., Stoy, M., Juchum, J., Davies, R. and Baxley, R., "A New Large-scale Process for Taxol and Related Taxanes from Taxus brevifolia," Pharm. Res., 12, 1003-1010(1995). https://doi.org/10.1023/A:1016206314225
  5. Choi, H. K., Son, J. S., Na, G. H., Hong, S. S., Park, Y. S. and Song, J. Y., "Mass Production of Paclitaxel by Plant Cell Culture," J. Plant Biotechnol., 29, 59-62(2002). https://doi.org/10.5010/JPB.2002.29.1.059
  6. Baloglu, E. and Kingston, D. G. I., "A New Semisynthesis of Paclitaxel from Baccatin III," J. Nat. Prod., 62, 1068-1071(1999). https://doi.org/10.1021/np990040k
  7. Lee, C. G. and Kim, J. H., "Optimization of Adsorbent Treatment Process for the Purification of Paclitaxel from Plat Cell Cultures of Taxus chinensis," Korean Chem. Eng. Res., 52, 497-502(2014). https://doi.org/10.9713/kcer.2014.52.4.497
  8. Kim, J. H., Lim, C. B., Kang, I. S., Hong, S. S. and Lee, H. S., "The Use of a Decanter for Harvesting Biomass from Plant Cell Cultures," Korean J. Biotechnol. Bioeng., 15, 337-341(2000).
  9. Kim, G. J. and Kim, J. H., "A Simultaneous Microwave-assisted Extraction and Adsorbent Treatment Process Under Acidic Conditions for Recovery and Separation of Paclitaxel from Plant Cell," Korean J. Chem. Eng., 32, 1023-1028(2015). https://doi.org/10.1007/s11814-015-0075-1
  10. Pyo, S. H., Song, B. K., Ju, C. H., Han, B. H. and Choi, H. J., "Effects of Adsorbent Treatment on the Purification of Paclitaxel From Cell Cultures of Taxus chinensis and Yew Tree," Process Biochem., 40, 1113-1117(2005). https://doi.org/10.1016/j.procbio.2004.03.004
  11. Kim, J. H., Kang, I. S., Choi, H. K., Hong, S. S. and Lee, H. S., "A Novel Prepurification for Paclitaxel from Plant Cell Cultures," Process Biochem., 37, 679-682(2002). https://doi.org/10.1016/S0032-9592(01)00247-3
  12. Kim, J. H. and Hong, S. S., "Optimization of Extraction Process for Mass Production of Paclitaxel from Plant Cell Cultures," Korean J. Biotechnol. Bioeng., 15, 346-351(2000).
  13. Hyun, J. E. and Kim, J. H., "Microwave-assisted Extraction of Paclitaxel from Plant Cell Cultures," Korean J. Biotechnol. Bioeng., 23, 281-284(2008).
  14. Pyo, S. H., Park, H. B., Song, B. K., Han, B. H. and Kim, J. H., "A Large-scale Purification of Paclitaxel from Cell Cultures of Taxus chinensis," Process Biochem., 39, 1985-1991(2004). https://doi.org/10.1016/j.procbio.2003.09.028
  15. Kim, J. H., "Optimization of Liquid-liquid Extraction Conditions for Paclitaxel Separation from Plant Cell Cultures," KSBB J., 24, 212-215(2009).
  16. Pachaiyappan, A., Varathan, S. and Venugopal, R., "Sonication Effect on the Reaction of 4-bromo-1-methylbenzene with Sodium Sulfide in Liquid-liquid Multi-site Phase-transfer Catalysis Condition - Kinetic Study," Ultrason. Sonochem., 23, 156-164(2015). https://doi.org/10.1016/j.ultsonch.2014.08.006
  17. Guo, Y. X., Han, J., Zhang, D. Y., Wang, L. H. and Zhou, L. L., "An Ammonium Sulfate/ethanol Aqueous Two-phase System Combined with Ultrasonication for the Separation and Purification of Lithospermic Acid B from Salvia miltiorrhiza Bunge," Ultrason. Sonochem., 19, 719-724(2012). https://doi.org/10.1016/j.ultsonch.2011.12.017
  18. Reza, R., Rouhollah, H. and Ahmad, L., "Ultrasound and Salt-assisted Liquid-liquid Extraction as an Efficient Method for Natural Product Extraction," Anal. Methods., 7, 3253(2015). https://doi.org/10.1039/C5AY00150A
  19. Ming, G., Huili, W., Meiping, M., Yuna, Z., Xiaohan, Y., Randy D., Dongli, D. and Xuedong, W., "Optimization of a Phase Separation Based Magnetic-stirring Salt-induced Liquid-liquid Microextraction Method for Determination of Fluoroquinolones in Food," Food Chem., 175, 181-188(2015). https://doi.org/10.1016/j.foodchem.2014.11.132
  20. Choi, H. K., Adams, T. L., Stahlhut, R. W., Kim, S. I., Yun, J. H., Song, B. K., Kim, J. H., Song, J. S., Hong, S. S. and Lee, H. S., "Method for Mass Production of Taxol by Semi-continuous Culture with Taxus chinensis Cell Culture," US. Patent No. 5,871,979(1999).
  21. Findik, S. and Gunduz, G., "Sonolytic Degradation of Acetic Acid in Aqueous Solutions," Ultrason. Sonochem., 14, 157-162(2007). https://doi.org/10.1016/j.ultsonch.2006.03.009
  22. Mukesh, G., Hu, H., Arun, S. M. and Madhumita, B, R., "Sonochemical Decomposition of Volatile and Non-volatile Organic Compounds a Comparative Study," Water Res., 38, 4247-4261(2004). https://doi.org/10.1016/j.watres.2004.08.008
  23. Jagannathan, T. K., Nagarajan, R. and Ramamurthi, K., "Effect of Ultrasound on Bubble Breakup Within the Mixing Chamber of An Effervescent Atomizer," Chem. Eng. Process., 50, 305-315 (2011). https://doi.org/10.1016/j.cep.2011.01.006
  24. Ha, G. S. and Kim, J. H., "Liquid-liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures by Adding Inorganic Salts," Korean Chem. Eng. Res., In press(2015).
  25. Eiji, I., Masako, N., Nobuyuki, K. and Kenji, K., "Synergy Effect of Ultrasonication and Salt Addition on Settling Behaviors of Activated Sludge," Sep. Purif. Technol., 144, 177-185(2015). https://doi.org/10.1016/j.seppur.2015.02.028
  26. Cesar, O., Ana, P., Jose, V. G., Antonio, M. and Juan, A. C., "Influence of High Intensity Ultrasound Application on Mass Transport, Microstructure and Textural Properties of Pork Meat (Longissimus dorsi) Brined at Different NaCl Concentrations," J. Food Eng., 119, 84-93(2013). https://doi.org/10.1016/j.jfoodeng.2013.05.016

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  4. Taxus chinensis 유래 파클리탁셀 정제를 위한 초음파를 이용한 마이셀 추출 vol.59, pp.1, 2016, https://doi.org/10.9713/kcer.2021.59.1.106