Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Identification of Major Component from Roots of Potentilla chinensis

딱지꽃(Potentilla chinensis) 뿌리 추출물의 주요성분 분리동정

  • Jung, Hae Soo (Anti-aging Research Institute of BIO-FD&C Co., Ltd.) ;
  • Kim, Hyoung Shik (Anti-aging Research Institute of BIO-FD&C Co., Ltd.) ;
  • Lee, Jeong Hun (Anti-aging Research Institute of BIO-FD&C Co., Ltd.) ;
  • Moh, Seo Jin (Innoplant Agricultural Union Corp.) ;
  • Yeo, Jin Hui (Jeongseon Agricultural Technology & Extension Center) ;
  • Park, Gi won (Jeongseon Agricultural Technology & Extension Center) ;
  • Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co., Ltd.)
  • Received : 2015.10.01
  • Accepted : 2015.10.08
  • Published : 2016.03.31
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Abstract

One of oriental medicinal plants, Potentilla chinensis, has been used for anti-inflammation, hemostatic, decryption, and antipyretic. Especially, a root of Potentilla chinensis was used as important material for oriental medication. Although several kinds of bioactive component of Potentilla chinensis extract from stems and leaves were identified, the major component of Potentilla chinensis from roots is not well established. In this study, the root of Potentilla chinensis was extracted in different solvent system and analyzed by high performance liquid chromatography (HPLC). According to HPLC analysis, a major component was isolated and its physicochemical properties were evaluated by mass spectrometry and nuclear magnetic resonance. Based on these results, isolated compound was identified as 2,3,8-Tri-O-methylellagic acid. And quantification of 2,3,8-Tri-O-methylellagic acid with different extraction solvent system was performed for industrial application.

딱지꽃(Potentilla chinensis)은 약초식물의 하나로 항염, 지혈, 해독 그리고 해열 등의 효과가 알려져 있다. 특히 딱지꽃의 뿌리는 약제로써 중요한 가치를 지니고 있다. 그러나 기존에 딱지꽃의 줄기나 잎에 대한 유효성분에 대한 연구가 시도된 반면 딱지꽃의 뿌리의 주요성분을 분석한 사례가 없었다. 따라서 본 연구는 딱지꽃의 뿌리를 다양한 용매별로 추출하고 주요한 물질을 분리정제 하였다. 분리 정제된 물질을 NMR과 mass 분석을 통하여 물질을 동정하였고 그 결과 주요성분이 화합물 (1)임을 확인하였다. 또한, 동정된 물질의 산업적 응용을 위해 딱지꽃 뿌리에서 용매별로 화합물 (1) 추출양을 정량하였다.

Keywords

References

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