Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Complete Sequence Analysis of a Korean Isolate of Chinese Yam Necrotic Mosaic Virus and Generation of the Virus Specific Primers for Molecular Detection

  • Kwon, Sun-Jung (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cho, In-Sook (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Seung-Kook (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Yoon, Ju-Yeon (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Gug-Seoun (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • Received : 2016.06.08
  • Accepted : 2016.09.08
  • Published : 2016.09.30
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Abstract

Chinese yam necrotic mosaic virus (CYNMV) is one of the most widespread viruses in Chinese yam (Dioscorea opposita Thunb.) and causes serious yield losses. Currently, genetic information of CYNMV is very restricted and complete genome sequences of only two isolates (one from Japan and another from China) have been reported. In this study, we determined complete genome sequence of the CYNMV isolate AD collected from Andong, Korea. Genetic analysis of the polyprotein amino acid sequence revealed that the Korean isolate AD has high similarity with the Japanese isolate PES3 (97%) but relatively low similarity with the Chinese isolate FX1 (78%). Phylogenetic analysis using the CYNMV 3' proximal nucleotide sequences harboring the coat protein and 3' untranslated region further supported genetic relationship among the CYNMV isolates. Based on comparative analysis of the CYNMV genome sequences determined in this study and other previous studies, we generated molecular detection primers that are highly specific and efficient for CYNMV diagnosis.

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References

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