Journal of Dairy Science and Biotechnology

  • 제34권4호
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  • Pages.231-237
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  • 2016
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  • 2733-4554(pISSN)
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  • 2733-4562(eISSN)
한국낙농식품응용생물학회 (Korean Society of Dairy Science and Biotechnology)
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Evaluation and Determination of Lactase Activity on Various Lactic Acid Bacteria isolated from Kefir by using HPLC

  • Jeong, Dana (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Dong-Hyeon (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Chon, Jung-Whan (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Hyunsook (Dept. of Food & Nutrition, College of Human Ecology, Hanyang University) ;
  • Lee, Soo-Kyung (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Hong-Seok (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Song, Kwang-Young (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Kang, Il-Byung (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Young-Ji (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Park, Jin-Hyeong (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Chang, Ho-Seok (Center for One Health, College of Veterinary Medicine, Konkuk University) ;
  • Seo, Kun-Ho (Center for One Health, College of Veterinary Medicine, Konkuk University)
  • 투고 : 2016.10.18
  • 심사 : 2016.11.01
  • 발행 : 2016.12.30
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초록

Kefir is a probiotic food. Probiotics have shown to be beneficial to health, and are currently of great interest to the food industry. Hence, this study was carried out to evaluate the lactase activity of kefir-isolated lactic acid bacteria. Three strains, Lactobacillu kefiri DH5 isolated from the kefir grains and Bifidobacterium animalis subsp. Lactis and Bifidobacteria longum 720, commercial probiotic LAB, were fermented in 10% reconstituted nonfat dry milk suspensions and incubated at $37^{\circ}C$ for 48 h, and then analyzed for various saccarides by HPLC. The results showed that changes in the concentrations of lactose and galactose were significantly decreased and increased, respectively (p<0.05), but all 3 probiotic strains tested in this study showed no increase in glucose concentration during 48 h of incubation. Both DH5 and BL720 showed high lactase activities (p<0.05), whereas BLC exhibited the lowest activity. Additionally, all three lactic acid bacteria showed high tagatose, but did not show high xylose and sedoheptulose. Finally, DH5, a kefir-isolated LAB, may have similar characteristics and properties to typical Bifidobacterium spp. and showed higher lactase activity than commercial Bifidobacterium spp.

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참고문헌

  1. Ahmed, Z., Wang, Y., Ahmad, A., Khan, S. T., Nisa, M., Ahmad, H. and Afreen, A. 2013. Kefir and health: A contemporary perspective. Crit. Rev. Food Sci. Nutr. 53:422-434. https://doi.org/10.1080/10408398.2010.540360
  2. Del Carmen, S., de Moreno de LeBlanc, A. and LeBlanc, J. G. 2016. Development of a potential probiotic yoghurt using selected anti-inflammatory lactic acid bacteria for prevention of colitis and carcinogenesis in mice. J. Appl. Microbiol.doi:10.1111/jam.13213.
  3. Frengova, G. I., Simova, E. D., Beshkova, D. M. and Simov, Z. I. 2000. Production and monomer composition of exopolysaccharides by yogurt starter cultures. Can. J. Microbiol. 46:1123-1127. https://doi.org/10.1139/w00-103
  4. Gamba, R. R., De Antoni, G. and Pelaez, A. L. 2016. Whey permeate fermented with kefir grains shows antifungal effect against Fusarium graminearum. J. Dairy Res. 83:249-255. https://doi.org/10.1017/S0022029916000121
  5. Gobinath, D. and Prapulla, S. G. 2014. Permeabilized probiotic Lactobacillus plantarum as a source of ${\beta}$-galactosidase for the synthesis of prebiotic galactooligosaccharides. Biotechnol. Lett. 36:153-157. https://doi.org/10.1007/s10529-013-1345-9
  6. Guzel-Seydim, Z., Kok-Tas, T. and Greene, A. K. 2011. Review: Functional properties of kefir. Crit. Rev. Food Sci. 51:261-268. https://doi.org/10.1080/10408390903579029
  7. Khoder, G., Al-Menhali, A. A., Al-Yassir, F. and Karam, S. M. 2016. Potential role of probiotics in the management of gastric ulcer. Exp. Ther. Med. 12:3-17. https://doi.org/10.3892/etm.2016.3293
  8. Kim, D. H., Chon, J. W., Kim, H., Kim, H. S., Choi, D., Hwang, D. G. and Seo, K. H. 2015. Detection and enumeration of lactic acid bacteria, acetic acid bacteria and yeast in kefir grain and milk using quantitative real-time PCR. J. Food Safety 35:102-107. https://doi.org/10.1111/jfs.12153
  9. Kim, D. H., Hwang, D. G., Chon, J. W., Kim, H., Kim, H. S., Song, K. Y., Yim, J. H., Kim, Y. J., Kang, I. B., Lee, S. K. and Seo, K. H. 2015. Simultaneous determination of carbohydrates and organic acids in various cultured dairy foods by high-performance liquid chromatography: A Preliminary Study. J. Milk Sci. Biotechnol. 33:263-269.
  10. Ianeva, O. D., Sichkar', S. V., Voronina, A. A. and Podgorskii, V. S. 2012. Selection of thermophilic lactose-fermenting yeast strains. Mikrobiol. Z. 74:65-70.
  11. Liu, G. X., Kong, J., Lu, W. W., Kong, W. T., Tian, H., Tian, X. Y. and Huo, G. C. 2011. ${\beta}$-Galactosidase with transgalactosylation activity from Lactobacillus fermentum K4. J. Dairy Sci. 94:5811-5820. https://doi.org/10.3168/jds.2011-4479
  12. O'Brien, K. V., Aryana, K. J., Prinyawiwatkul, W., Ordonez, K. M. and Boeneke, C. A. 2016. Short communication: The effects of frozen storage on the survival of probiotic microorganisms found in traditionally and commercially manufactured kefir. J. Dairy Sci. doi:10.3168/jds.2015-10284.
  13. Pidoux, M., Marshall, V. M., Zanoni, P. and Brooker, B. 1990. Lactobacilli isolated from sugary kefir grains capable of polysaccharide production and minicell formation. J. Appl. Bacteriol. 69:311-320. https://doi.org/10.1111/j.1365-2672.1990.tb01521.x
  14. Prado, M. R., Boller, C., Zibetti, R. G., de Souza, D., Pedroso, L. L. and Soccol, C. R. 2016. Anti-inflammatory and angiogenic activity of polysaccharide extract obtained from Tibetan kefir. Microvasc. Res. 108:29-33. https://doi.org/10.1016/j.mvr.2016.07.004
  15. Rababah, T. M., Awaisheh, S. S., Al-Tamimi, H. J. and Brewer, S. 2015. The hypocholesterolemic and hormone modulation effects of isoflavones alone or co-fermented with probiotic bacteria in hyper cholesterolemic rats model. Int. J. Food Sci. Nutr. 66:546-552. https://doi.org/10.3109/09637486.2015.1028908
  16. Rahimzadeh, G., Bahar, M. A. and Mozaffari, N. A. 2012. Antimicrobial activity Kefir on different time fermentation. Iran J. Med. Microbiol. 5:35-41.
  17. Samah, S., Ramasamy, K., Lim, S. M. and Neoh, C. F. 2016. Probiotics for the management of type 2 diabetes mellitus: A systematic review and meta-analysis. Diabetes Res. Clin. Pract. 18:172-182.
  18. Shin, S. M., Song, K. Y., Seo, K. H. and Yoon, Y. C. 2012. Study on the quality of yoghurt made from soymilk during storage. J. Korean Dairy Technol. and Sci. 30:83-92.
  19. Splechtna, B., Nguyen, T. H., Steinbock, M., Kulbe, K. D., Lorenz, W. and Haltrich, D. 2006. Production of prebiotic galacto-oligosaccharides from lactose using beta-galactosidases from Lactobacillus reuteri. J. Agric. Food Chem. 54: 4999-5006. https://doi.org/10.1021/jf053127m
  20. Yoon, Y. C., Song, K. Y., Jeong, D. K. and Jeon, I. J. 2000. Assessment of potential ${\beta}$-galactosidase activities of Bifidobacteria cultures by high performance liquid chromatography. Food Sci. Biotechnol. 9:43-47.
  21. Yu, J., Wang, H. M., Zha, M. S., Qing, Y. T., Bai, N., Ren, Y., Xi, X. X. and Liu, W. J. 2015. Molecular identification and quantification of lactic acid bacteria in traditional fermented dairy foods of Russia. J. Dairy Sci. 98:5143-5154. https://doi.org/10.3168/jds.2015-9460
  22. Zavala, L., Golowczyc, M. A., van Hoorde, K., Medrano, M., Huys, G., Vandamme, P. and Abraham, A. G. 2016. Selected Lactobacillus strains isolated from sugary and milk kefir reduce Salmonella infection of epithelial cells in vitro. Benef. Microbes. 13:1-12.