Journal of Plant Biotechnology

  • 제43권4호
  • /
  • Pages.466-472
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  • 2016
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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SSR 마커를 이용한 배 유전자원의 유연관계

Genetic relationships of pear germplasms using simple sequence repeat marker

  • 천재안 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 조강희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김세희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 이한찬 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 최인명 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 박서준 (농촌진흥청 국립원예특작과학원 과수과)
  • Chun, Jae An (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Han-Chan (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Choi, In Myong (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Seo Jun (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 투고 : 2016.10.19
  • 심사 : 2016.11.14
  • 발행 : 2016.12.31
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초록

본 연구는 15개의 SSR 마커를 이용하여 배 유전자원 115점에 대한 유전적 다양성을 분석하였다. 분석된 마커당 대립유전자수는 3 ~ 41개로 평균 16개였으며, 마커의 이용도는 평균 0.966이었다. $H_{obs}$는 0.140 ~ 0.929로 평균 0.603이었으며 $H_{exp}$는 0.463~0.904로 평균 0.718로 분석되었다. PIC 값은 0.403 ~ 0.897의 범위에 속하였으며 평균값은 0.692 이었다. 배 유전자원의 유전적 거리에 따라 계통간 유연관계를 분석한 결과 지리적 분포와 유전적 특성에 의해 크게 2개의 그룹으로 구분되었다. 첫 번째 그룹은 유럽종 P. communis에 속하는 품종으로 구성되었으며 두 번째 그룹은 동양배 그룹으로 P. pyrifolia, P. ussuriensis, P. bretschneideri, P. betulaefolia, P. calleryana와 교잡종 및 종의 분류가 명확하지 않은 유전자원이 포함되었다. 본 연구는 배 유전자원의 유전자형 분석을 통하여 유전적 다양성을 가지는 품종들을 분류하였으며, 육종을 위한 기초자료로 활용 가능할 것으로 사료된다.

This study analyzed the genetic diversity of 115 pear germplasms using 15 SSR markers. Three to forty-one SSR alleles were detected for each locus with an average of 16 alleles per locus. The average availability of markers was 0.966. The average observed heterozygosity ($H_{obs}$) was 0.603 (range: 0.140 to 0.929). The average expected heterozygosity ($H_{exp}$) was 0.718 (range: 0.463 to 0.904). The average polymorphism information content (PIC) was 0.692 (range: 0.403 to 0.897). The genetic relationships of pear germplasms were classified into two major groups by geographic origins and genetic characteristics according to genetic distance. The first group was composed of European pear belonging to Pyrus communis. The second group consisted of P. pyrifolia, P. ussuriensis, P. bretschneideri, P. betulaefolia, P. calleryana, interspecific hybrids, and unclear germplasms. The results of this study suggest that genotype analysis of pear germplasms using SSR markers can identify the genetic diversity of germplasms, and can be used to provide basic information for pear breeding.

키워드

참고문헌

  1. Barkley NA, Roose ML, Krueger RR, Federici CT (2006) Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor Appl Genet 112:1519-1531 https://doi.org/10.1007/s00122-006-0255-9
  2. Bell RL (1990) Pears (Pyrus). In: Moore JN, Ballington JR Jr (eds) Genetic resources of temperate fruit and nut Crops I. International Society for Horticultural Science, Wageningen, the Netherlands, pp 655-697
  3. Bell RL, Quamme HA, Layne REC, Skirvin RM (1996) Pears. In: Janick, Moore JN (eds), Fruit breeding, Vol I : Tree and tropical fruits. John Willey & Sons, London, pp 441-514
  4. Cao Y, Tian L, Gao Y, Liu F (2012) Genetic diversity of cultivated and wild Ussurian Pear (Pyrus ussuriensis Maxim.) in China evaluated with M13-tailed SSR markers. Genet Resour Crop Evol 59:9-17 https://doi.org/10.1007/s10722-011-9661-1
  5. Dirlewanger E, Cosson P, Tavaud M, Aranzana M, Poizat C, Zanetto A, Arus P, Laigret F (2002) Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.). Theor Appl Genet 105:127-138 https://doi.org/10.1007/s00122-002-0867-7
  6. Emanuelli F, Lorenzi S, Grzeskowiak L, Catalano V, Stefanini M, Troggio M, Myles S, Martinez-Zapater JM, Zyprian E, Moreira FM (2013) Genetic diversity and population structure assessed by SSR and SNP markers in a large germplasm collection of grape. BMC Plant Biol 13:39 https://doi.org/10.1186/1471-2229-13-39
  7. Hokanson, S., W. Lamboy, A. Szewc-McFadden, and J. McFerson. 2001. Microsatellite (SSR) variation in a collection of Malus (apple) species and hybrids. Euphytica 118:281-294 https://doi.org/10.1023/A:1017591202215
  8. Kim DI (1998) Taxonomy of oriental pear (Pyrus sp.) based on multivariate and RAPD analysis. Ph.D. Thesis. Seoul Natl Univ pp 81-82
  9. Kim MS, Kim SH, Han JG, Song JH, Kim HS, Kim DH, Kwon YS (2015) Genetic diversity of wild pear accessions collected in Korea. Korean J Breed Sci 47:45-53 https://doi.org/10.9787/KJBS.2015.47.1.045
  10. Kimura T, Shi YZ, Shoda M, Kotobuki K, Matsuta N, Hayashi T, Ban Y, Yamamoto T (2002) Identification of Asian pear varieties by SSR analysis. Breed Sci 52:115-121 https://doi.org/10.1270/jsbbs.52.115
  11. Liebhard R, Gianfranceschi L, Koller B, Ryder C, Tarchini R, Van de Weg E, Gessler C (2002) Development and characterisation of 140 new microsatellites in apple (Malus ${\times}$ domestica Borkh.). Mol Breed 10:217-241 https://doi.org/10.1023/A:1020525906332
  12. Lui K, Muse S (2005) PowerMarker: integrated analysis environment for genetic marker data. Bioinformatics 21:2128-2129 https://doi.org/10.1093/bioinformatics/bti282
  13. Nishitani C, Terakami S, Sawamura Y, Takada N, Yamamoto T (2009) Development of novel EST-SSR markers derived from Japanese pear(Pyrus pyrufolia). Breed Sci 59:391-400 https://doi.org/10.1270/jsbbs.59.391
  14. Phillips RL, Vasil IK (2001) DNA-based markers in plants. Vol. 6. Springer Science & Business Media
  15. Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2:225-238 https://doi.org/10.1007/BF00564200
  16. Sawamura Y, Takada N, Yamamoto T, Saito T, Kimura T, Kotobuki K (2008) Identification of Parent-offspring Relationships in 55 Japanese Pear Cultivars Using S-RNase Allele and SSR markers. J Jpn Soc Hortic Sci 77:364-373 https://doi.org/10.2503/jjshs1.77.364
  17. Sehic J, Garkava-Gustavsson L, Fernandez-Fernandez F, Nybom H (2012) Genetic diversity in a collection of European pear (Pyrus communis) cultivars determined with SSR markers chosen by ECPGR. Sci Hortic 145:39-45 https://doi.org/10.1016/j.scienta.2012.07.023
  18. Silfverberg-Dilworth E, Matasci CL, Van de Weg WE, Van Kaauwen MPW, Walser M, Kodde LP, Soglio V, Gianfranceschi L, Durel CE, Costa F, Yamamoto T, Koller B, Gessler C, Patocchi A (2006) Microsatellite markers spanning the apple (Malus x domestica Borkh.) genome. Tree Genet. Genomes 2:202-224 https://doi.org/10.1007/s11295-006-0045-1
  19. Sneath PHA, Sokal RR (1973) Numerical taxonomy : The principles and practice of numerical classification, W. H. Freeman, San Francisco
  20. Teng Y, Tanabe K, Tamura F, Itai A (2002) Genetic relationships of Pyrus species and cultivars native to East Asia revealed by randomly amplified polymorphic DNA markers. J Am Soc Hortic Sci 127:262-270
  21. Wunsch A, Hormaza J (2007) Characterization of variability and genetic similarity of European pear using microsatellite loci developed in apple. Sci Hortic 113:37-43 https://doi.org/10.1016/j.scienta.2007.02.002
  22. Weber JL, May PE (1989) Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet 44:388-396
  23. Yamamoto T, Kimura T, Sawamura Y, Kotobuki K, Ban Y, Hayashi T, Matsuta N (2001) SSRs isolated from apple can identify polymorphism and genetic diversity in pear. Thero Appl Genet 102:865-870 https://doi.org/10.1007/s001220000524
  24. Yamamoto T, Kimura T, Sawamura Y, Manabe T, Kotobuki K, Hayashi T, Ban Y, Matsuta N (2002a) Simple sequence repeats for genetic analysis in pear. Euphytica 124:129-137 https://doi.org/10.1023/A:1015677505602
  25. Yamamoto T, Kimura T, Shoda M, Ban Y, Hayashi T, Matsuta N (2002b) Development of microsatellite markers in the Japanese pear (Pyrus pyrifolia Nakai). Mol Ecol Not 2:14-16 https://doi.org/10.1046/j.1471-8286.2002.00128.x