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제약회사 폐수처리장 방류수 중 항생제 내성 Enterobacteria에 관한 연구

Study on Antibiotic Resistant Enterobacteria in Pharmaceutical Effluent

  • 김재건 (신한대학교 에너지환경공학과) ;
  • 김영진 (동남보건대학교 환경보건과)
  • Kim, Jae-Gun (Department of Energy and Environment Engineering, Shinhan University) ;
  • Kim, Young Jin (Department of Environmental Health, Dongnam Health University)
  • 투고 : 2015.12.07
  • 심사 : 2016.02.15
  • 발행 : 2016.02.29

초록

Objectives: This study aims to examine the concentration, diversity, and antibiotic characteristics of penicillin G resistant enterobacteria present in pharmaceutical effluent. Methods: Water sampling was performed from a pharmaceutical company in Gyeonggi-do Province, Korea in March 2015. Water samples were plated in triplicate on tryptic soy agar plates with 32 mg/L of penicillin G. Penicillin G resistant enterobacteria were selected from the effluent and were subjected to 16S rRNA analysis for penicillin G resistant species determination. Identified resistant strains were tested for resistance to various antibiotics. Results: Penicillin G resistant enterobacteria were present at 6.2% as to culturable heterotrophic bacteria. Identified penicillin G resistant enterobacteria exhibited resistance to more than 10 of the antibiotics studied. These resistant bacteria are gram negative and are closely related to pathogenic species. Conclusion: Multi-antibiotic resistant bacteria in the effluent suggest a need for disinfection and advanced oxidation processes for pharmaceutical effluents.

키워드

참고문헌

  1. Available: http://en.wikipedia.org/wiki/Enterobactereaceae[accessed 19 March]
  2. Ministry of Environment. Available: http://www.law.go.kr/DRF/lawService.do?OC=jaa806&target=law&MST=166074&type=HTML&mobileYn=&efYd=20150101 ?accessed 19 May 2015?
  3. Kummerer K. Significance of antibiotics in the environment. Journal of Antimicrobial Chemotherapy. 2003; 52: 5-7. https://doi.org/10.1093/jac/dkg293
  4. Larsson DGK. Pollution from drug manufacturing: review and perspectives. Available: http://rstb.royalssocietypublishing.org [assessed 9 February 2015]
  5. National Institute of Environmental Research. Available: http://img.kisti.re.kr/originalView/originalView.jsp [accessed 20 May 2015]
  6. Andreozzi R, Raffaele M, Nicklas P. Pharmapeutical in STP effluents and their solar photodegradation in aquatic environment. CHEMOSPHERE. 2003; 50: 1319-1330. https://doi.org/10.1016/S0045-6535(02)00769-5
  7. Larsson DGK, Pedro CD, Paxcus N. Effluent from drug manufactures contains extremely high levels of pharmaceuticals. Journal of Hazardous materials. 2007; 148: 751-755. https://doi.org/10.1016/j.jhazmat.2007.07.008
  8. National Institute of Environmental Research. Available: http://qaqc.nier.go.kr/qaqcnew/standard/standardlist.do [accessed 20 May 2015]
  9. Available: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Fluka/Datasheet/70186dat.pdf [accessed 21 May 2015]
  10. Clinical Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing, 23rd informational supplement: M100-S23. Wayne, PA: Clinical Laboratory Standards Institute; 2013.
  11. Kim YJ, Kim JO. Study on Oxytetracycline Resistant Bacteria in the surface water Environment. J Environ Health Sci. 2015; 41(1): 44-48.
  12. QIAGEN. DNeasy${(R)}$ Blood & Tissue Handbook. Available: https://www.qiagen.com/kr/resources/resourcedetail?id=6b09dfb8-6319-464d-996c-79e8c7045a50&lang=en [assessed 12 February 2016]
  13. Giobannoni SJ, Rappe MS, Vergin KL, Adair NL. 16S rRNA genes reveal stratified open ocean bacterioplankton populations related to the Green Non-Sulfur bacteria. Proc. Natl. Acad. Sci. 1996; 93: 7979-7984. https://doi.org/10.1073/pnas.93.15.7979
  14. Sutter VL, Kwock YY, Finegold SM. Susceptibility of Bacteroides fragilis to Six Antibiotics Determined by Standardized Antimicrobial Disc Susceptibility Testing. Antimicrob. Agents Chemother. 1973; 3(2): 188-193. https://doi.org/10.1128/AAC.3.2.188
  15. Finlay JE, Miller LA, Poupard JA. Interpretive criteria for testing susceptibility of staphylococci to mupirocin. Antimicrob. Agents Chemother. 1997; 41(5): 1137-1139.
  16. Oh HK, Park JH. Characteristics of Antibiotic Resistant Bacteria in Urban Sewage and River. Journal of KSEE. 2009; 31(3): 232-239.
  17. Available: https://en.wikipedia.org/wiki/Enterobacter [accessed 8 July 2015].
  18. Enterobacter Infections. Available: http://emedicine.mediscape.com/article/216845-overviw [accessed 21 October 2015].
  19. Hossain A, Ferraro MJ, Pino RM, Dew RB, Moland ES, Lockhart TJ, et al. Plasmid-Mediated Carbapenem-Hydrolyzing Enzyme KPC-2 in an Enterobacter sp. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2004; 48(11): 4438?4440. https://doi.org/10.1128/AAC.48.11.4438-4440.2004
  20. Brisse S, Milatovie D, Fluit AC, Verhoef J, Schmitz FJ. Epidermiology of Quinolone Resistant of Klebsiella pneumoniae and Klebsiella oxytoca in Europe. Eur J. Clin Mircobio Infect Dis. 2000; 19: 64-68. https://doi.org/10.1007/s100960050014
  21. Brisse S, Grimont F, Grimont PAD. The Genus Klebsiella. Procaryotes. 2006; 6: 159-196.
  22. Yigit H, Queenan AM, Rasheed JK, Biddle JW, Domenech-Sanchez A, Alberti S, et al. Carbapenen-Resistnat Strain of Klebsiella oxytoca Harboring Carbapenem-Hydrolysing ${\beta}$-Latamase KPC-2. ANTIMICROB. AGENTS CHEMOTHER. 2003; 47(12): 3881-3889. https://doi.org/10.1128/AAC.47.12.3881-3889.2003
  23. Brisse S, Milatovic D, Fliit AD C, Verhoef J, Martin N, Sheuring S, et al. Compartive In Vitro Activities of Ciprofloxan, Clinafloxacin, Gatifloxacin, Levofloxacin, Moxifloxacin, and Trovafloxacin against Klebsiella pneumonia, Klebsiella oxytoca, Enterbacter cloacae, and Enterobacter aerogenes Clinical Isolates with Alterations in GyrA and ParC Proteins. ANTIMICROB. AGENTS CHEMOTHER. 1999; 43(8): 2051-2055.
  24. Kim YJ, Kim JG, Kim JO. Study on Antibiotic Resistant bacteria in Hospital Effluents. J. Korea Society of Environmental Administration. 2015; 21(3): 23-30.
  25. Available : http://news.joins.com/article/19094395 [accessed 20 November 2015]
  26. Larsson DGJ, Pedro CD, Paxeus N. Effluent from drug manufactures contains extremely high levels of pharmaceuticals. Journal of Hazardous Materials. 2007; 148: 751-755. https://doi.org/10.1016/j.jhazmat.2007.07.008
  27. Hernando MD, Mezcua M, Fernandez-Alba AR, Barcelo D. Enviornmental risk assessment of pharmaceutical residues in wastewater effluents, surface waters and sediments. Talanta. 2006; 69: 334-342. https://doi.org/10.1016/j.talanta.2005.09.037
  28. G. Hey G, Grabic R, Ledin A, Jansen JLC, Andeson HR. Oxidation of pharmaceuticals by chlorine dioxide in biologically treated wastewater. Chemical Engineering Journal. 2012; 185(5): 236-242.
  29. Santiago Esplugas S, Bila DM, Krause LGT, Dezotti M. Ozonation and advanced oxidation technologies to remove endocrine disrupting chemicals (EDCs) and pharmaceuticals and personal care products (PPCPs) in water effluents. Journal of Hazardous Materials. 2007; 149(3): 631-642. https://doi.org/10.1016/j.jhazmat.2007.07.073