Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic Prokaryotic Diversity in Boring Slime from the Development of a Groundwater Heat Pump System

지하수 히트펌프 시스템의 지중 환경관리를 위한 시추 슬라임의 원핵생물 유전자 다양성

  • Kim, Heejung (School of Earth and Environmental Sciences, Seoul National University) ;
  • Lee, Siwon (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Park, Junghee (School of Earth and Environmental Sciences, Seoul National University) ;
  • Joun, Won-Tak (School of Earth and Environmental Sciences, Seoul National University) ;
  • Kim, Jaeyeon (School of Earth and Environmental Sciences, Seoul National University) ;
  • Kim, Honghyun (School of Earth and Environmental Sciences, Seoul National University) ;
  • Lee, Kang-Kun (School of Earth and Environmental Sciences, Seoul National University)
  • 김희정 (서울대학교 지구환경과학부) ;
  • 이시원 (국립환경과학원 상하수도연구과) ;
  • 박정희 (서울대학교 지구환경과학부) ;
  • 전원탁 (서울대학교 지구환경과학부) ;
  • 김재연 (서울대학교 지구환경과학부) ;
  • 김홍현 (서울대학교 지구환경과학부) ;
  • 이강근 (서울대학교 지구환경과학부)
  • Received : 2016.06.27
  • Accepted : 2016.11.02
  • Published : 2016.12.28
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Abstract

Groundwater heat pump (GWHP) systems must consider phenomena such as clogging to improve system efficiency and maintenance. In this study, we evaluated the prokaryotic diversity in a boring slime sample obtained at a depth of 10 m, which represented an undisturbed sample not affected by aquifer drawdown. Bacteria belonging to the phyla Proteobacteria (20.8%), Acidobacteria (18.8%), Chloroflexi (16.9%), and Firmicutes (10.2%) were found. Additionally, 144 species were identified as belonging to the genus Koribacter. Archaeal phyla were detected including Thaumarchaeota (42.8%), Crenarchaeota (36.9%), and Euryarchaeota (17.4%) and the class level comprised the miscellaneous Crenarchaeota group (MCG), Finnish forest soil type B (FFSB), and Thermoplasmata, which collectively accounted for approximately 69.4% of the detected Archaea. Operational taxonomic units (OTUs) were analyzed to reveal 3,565 bacterial and 836 archaeal OTUs, with abundances of 7.81 and 6.68, and richnesses of 5.96E-4 and 2.86E-3, respectively. The distribution of the groundwater microbial community in the study area showed a higher proportion of non-classified or unidentified groups compared to typical communities in surface water and air. In addition, 135 (approx. 1.9%) reads were assigned to a bacterial candidate associated with clogging.

지하수의 열을 이용한 히트펌프 시스템에서는 열 효율 유지를 위한 클로깅 현상이 고려되어야 한다. 클로깅 현상은 토양 지하수 환경에서 이화학적 요인 외에도 미생물학적 요인으로 발생한다. 이번 연구에서는 안정적인 지하수 열원 냉난방 시스템 운영을 위하여, 대수층 수위강하의 영향을 받지 않는 지하 10 미터 지점에서 불교란 시추 슬라임의 초기상태 원핵생물 다양성을 조사하였다. 세균은 문 수준에서 Proteobacteria (20.8%), Acidobacteria (18.8%), Chloroflexi (16.9%) 및 Firmicutes (10.2%) 등으로 나타났고, 속 수준에서는 Koribacter속 등 144개속이 분석되었다. 고세균은 문 수준에서 Thaumarchaeota (42.8%), Crenarchaeota (36.9%) 및 Euryarchaeota (17.4%)이 나타났으며, 강 수준에서 약 69.4% 비율로 Miscellaneous Crenarchaeota Group (MCG), Finnish Forest Soil Type B (FFSB) 및 Thermoplasmata가 분석되었다. Operational taxonomic units (OTUs)는 세균 3,565 및 고세균 836 OTUs로 나타났고, 세균이 고세균에 비해 풍부하며 우점도가 낮게 나타났다. 또한, 관정 막힘 현상을 유발할 가능성 있는 세균 후보군 135개(1.9%) reads가 분석되었으며, 향후 클로깅 현상에 대한 연구에 자료로 활용할 수 있을 것으로 기대된다.

Keywords

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