Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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The Anti-Inflammatory Effects of Picea wilsonii Mast on HaCaT Cells

HaCaT 세포주에서 Picea wilsonii Mast의 항염효과

  • 차경재 (서해대학교 임상병리과)
  • Received : 2016.10.28
  • Accepted : 2016.12.05
  • Published : 2016.12.31
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Abstract

Atopic dermatitis refers to a chronic, recurrent, skin condition, typically typified by itching, inflamed skin. It precedes other allergic diseases, such as asthma, food allergies, and allergic rhinitis, and is usually accompanied by various other immune disorders and secondary symptoms. In this study, we discovered that when treating TNF-${\alpha}$ and IFN-${\gamma}$-stimulated HaCaT cells with various concentrations of Picea wilsonii Mast (PwM) extracts, the cell viability was excellent. In addition, we measured the inflammatory cytokines associated with atopic dermatitis, including IL-6, IL-8, IL-13, and MCP-1. The production of IL-6, IL-13, and MCP-1 decreased in the presence of PwM extracts, whereas there was no significant difference in the production of IL-8. Further studies are necessary to develop an effective cure for atopic dermatitis and inflammation using foreign plant extracts, and PwM efficacy should be determined with an in-depth, objective verification process using protein and mechanism analysis.

아토피피부염은 가려움증을 보이는 만성의, 재발성의 염증성 피부상태를 말하며 천식, 식품 알레르기, 알레르기 비염 등과 같은 다른 알레르기 질환보다 먼저 발생하고 다양한 면역학적 이상과 이로 인한 다양한 속발 증상도 갖고 있는 만성 재발성의 습관성 질환이다. 본 연구는 TNF-${\alpha}$와 IFN-${\gamma}$로 유도된 HaCaT 세포주를 이용하여 농도별로 PwM 천연물을 처리하였을 때 세포생존율이 우수한 것을 확인하였으며 아토피피부염과 관련된 염증성 사이토카인 즉, IL-6, IL-8, IL-13, MCP-1을 측정하였다. 그 결과 IL-6, IL-13, MCP-1의 생산량은 PwM 천연물에 의해 감소하였고 IL-8은 유의한 변화가 없었다. 앞으로의 연구는 해외생물소재를 활용한 아토피피부염 및 염증에 효과적인 치료제 후보물질을 개발하는 것이며 단백질 분석과 기전 연구를 통해 보다 객관적인 검증이 더 필요하다고 사료된다.

Keywords

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