Molecules and Cells

  • 제39권2호
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  • Pages.163-168
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  • 2016
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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Caffeine Induces the Stress Response and Up-Regulates Heat Shock Proteins in Caenorhabditis elegans

  • Al-Amin, Mohammad (Department of Bioscience and Biotechnology and Institute of KU Biotechnology, Konkuk University) ;
  • Kawasaki, Ichiro (Department of Bioscience and Biotechnology and Institute of KU Biotechnology, Konkuk University) ;
  • Gong, Joomi (Department of Bioscience and Biotechnology and Institute of KU Biotechnology, Konkuk University) ;
  • Shim, Yhong-Hee (Department of Bioscience and Biotechnology and Institute of KU Biotechnology, Konkuk University)
  • 투고 : 2015.10.25
  • 심사 : 2015.10.30
  • 발행 : 2016.02.29
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초록

Caffeine has both positive and negative effects on physiological functions in a dose-dependent manner. C. elegans has been used as an animal model to investigate the effects of caffeine on development. Caffeine treatment at a high dose (30 mM) showed detrimental effects and caused early larval arrest. We performed a comparative proteomic analysis to investigate the mode of action of high-dose caffeine treatment in C. elegans and found that the stress response proteins, heat shock protein (HSP)-4 (endoplasmic reticulum [ER] chaperone), HSP-6 (mitochondrial chaperone), and HSP-16 (cytosolic chaperone), were induced and their expression was regulated at the transcriptional level. These findings suggest that high-dose caffeine intake causes a strong stress response and activates all three stress-response pathways in the worms, including the ER-, mitochondrial-, and cytosolic pathways. RNA interference of each hsp gene or in triple combination retarded growth. In addition, caffeine treatment stimulated a food-avoidance behavior (aversion phenotype), which was enhanced by RNAi depletion of the hsp-4 gene. Therefore, up-regulation of hsp genes after caffeine treatment appeared to be the major responses to alleviate stress and protect against developmental arrest.

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참고문헌

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