Molecules and Cells

  • 제39권2호
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  • Pages.141-148
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  • 2016
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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De Novo Transcriptome Analysis of Cucumis melo L. var. makuwa

  • Kim, Hyun A (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Shin, Ah-Young (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Min-Seon (Nongwoo Bio Co., Ltd.) ;
  • Lee, Hee-Jeong (Nongwoo Bio Co., Ltd.) ;
  • Lee, Heung-Ryul (Nongwoo Bio Co., Ltd.) ;
  • Ahn, Jongmoon (Nongwoo Bio Co., Ltd.) ;
  • Nahm, Seokhyeon (Nongwoo Bio Co., Ltd.) ;
  • Jo, Sung-Hwan (SEEDERS, Daeduk Industry Academic Cooperation Building) ;
  • Park, Jeong Mee (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Suk-Yoon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 투고 : 2015.10.02
  • 심사 : 2015.11.02
  • 발행 : 2016.02.29
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초록

Oriental melon (Cucumis melo L. var. makuwa) is one of six subspecies of melon and is cultivated widely in East Asia, including China, Japan, and Korea. Although oriental melon is economically valuable in Asia and is genetically distinct from other subspecies, few reports of genome-scale research on oriental melon have been published. We generated 30.5 and 36.8 Gb of raw RNA sequence data from the female and male flowers, leaves, roots, and fruit of two oriental melon varieties, Korean landrace (KM) and Breeding line of NongWoo Bio Co. (NW), respectively. From the raw reads, 64,998 transcripts from KM and 100,234 transcripts from NW were de novo assembled. The assembled transcripts were used to identify molecular markers (e.g., single-nucleotide polymorphisms and simple sequence repeats), detect tissue-specific expressed genes, and construct a genetic linkage map. In total, 234 single-nucleotide polymorphisms and 25 simple sequence repeats were screened from 7,871 and 8,052 candidates, respectively, between the KM and NW varieties and used for construction of a genetic map with 94 F2 population specimens. The genetic linkage map consisted of 12 linkage groups, and 248 markers were assigned. These transcriptome and molecular marker data provide information useful for molecular breeding of oriental melon and further comparative studies of the Cucurbitaceae family.

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피인용 문헌

  1. Transcriptome analysis of the oriental melon (Cucumis meloL. var.makuwa) during fruit development vol.5, 2017, https://doi.org/10.7717/peerj.2834
  2. An improved assembly and annotation of the melon (Cucumis melo L.) reference genome vol.8, pp.1, 2018, https://doi.org/10.1038/s41598-018-26416-2
  3. Metabolomic analysis of the occurrence of bitter fruits on grafted oriental melon plants vol.14, pp.10, 2019, https://doi.org/10.1371/journal.pone.0223707