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Plasma Stress Responses in Juvenile Red-Spotted Grouper (Epinephelus akaara) exposed to Abrupt Salinity Decrease

  • Lee, Jang-Won (Dept. of Marine Biology, Pukyong National University) ;
  • Kim, Hyung Bae (Dept. of Marine Bio-resources, Gangwon Provincial College) ;
  • Baek, Hea Ja (Dept. of Marine Biology, Pukyong National University)
  • Received : 2016.07.01
  • Accepted : 2016.09.05
  • Published : 2016.09.30

Abstract

The objective of the current study was to determine acute plasma stress responses in two size groups of juvenile Epinephelus akaara (average body weight: $8.4{\pm}2.1$ and $3.3{\pm}0.6g$; 150 and 120 days after hatch, respectively) exposed to abrupt salinity drops (from 34 practical salinity unit, PSU seawater to 18, 10 PSU (experiment 1) or 26, 18, 10 PSU (experiment 2), respectively). Plasma glucose, glutamic oxalate transaminase, glutamic pyruvate transaminase, red blood cell counts, and gill histology were determined during 72 h exposure. Significantly increased plasma glucose, glutamic oxalate transaminase levels, and red blood cell counts were observed in fish exposed to 18 or 10 PSU. Histological changes, such as hyperplasia and lifting of epithelium in the gill secondary lamellae, were also observed in fish exposed to 18 or 10 PSU at 72 h post-drop. E. akaara exposed to sudden salinity drops to 18 or 10 PSU still seems to undergo the primary adjustment phase before fish reaches a new homeostasis, whereas fish exposed to 26 PSU seems to mount osmotic changes. Therefore, the no observed adverse effect levels for 72 h acute salinity challenge was 26 PSU in our study, and salinity drop to 18 PSU and below can possibly cause acute adverse effect, in which fish could be vulnerable to additional stresses such as a temperature changes or handling stress.

Keywords

References

  1. Akatsu S, Al-Abdul-Elah KM, Teng SK (1983) Effects of salinity and water temperature on the survival and growth of brown-spotted grouper larvae (Epinephelus tauvina, Serranidae). J World Aquacult Soc 14:624-635.
  2. Arjona FJ, Vargas-Chacoff L, Ruiz-Jarabo I, Goncalves O, Pascoa I, Martin Del Rio MP, Mancera JM (2009) Tertiary stress responses in Senegalese sole (Solea senegalensis Kaup, 1858) to osmotic challenge: Implications for osmoregulation, energy metabolism and growth. Aquaculture 287:419-426. https://doi.org/10.1016/j.aquaculture.2008.10.047
  3. Arjona FJ, Vargas-Chacoff L, Ruiz-Jarabo I, Martin Del Rio MP, Mancera JM (2007) Osmoregulatory response of Senegalese sole (Solea senegalensis) to changes in environmental salinity. Comp Biochem Physiol Part A 148:413-421. https://doi.org/10.1016/j.cbpa.2007.05.026
  4. Asztalos B, Nemcsok J, Benedeczky I, Gabriel R, Szabo A, Refaie OJ (1990) The effects of pesticides on some biochemical parameters of common carp (Cyprinus carpio). Arch Environ Contam Toxicol 19:275-282. https://doi.org/10.1007/BF01056097
  5. Bhattacharya H, Xiao Q, Lun L (2008) Toxicity study of nonylphenol on rosy barb (Puntius conchonious): A biochemical and histopathological evaluation. Tissue Cell 40:243-249. https://doi.org/10.1016/j.tice.2007.12.004
  6. Cho HC, Kim JE, Kim HB, Baek HJ (2015) Effects of water temperature change on the hematological responses and plasma cortisol levels in growing of red spotted grouper, Epinephelus akaara. Dev Reprod 19:19-24. https://doi.org/10.12717/DR.2015.19.1.019
  7. Cornish A (2003) Epinephelus akaara. The IUCN red list of threatened species. version 2015. 2; DOI: http://dx.doi.org/10.2305/IUCN.UK.2003.RLTS.T43974A10846282.en. accessed 28 October 2015.
  8. Datta S, Saha DR, Ghosh D, Majumdar T, Bhattacharya S, Mazumder S (2007) Sub-lethal concentration of arsenic interferes with the proliferation of hepatocytes and induces in vivo apoptosis in Clarias batrachus L. Comp Biochem Physiol C Toxicol Pharmacol 145:339-49. https://doi.org/10.1016/j.cbpc.2007.01.005
  9. Deng DF, Teh FC, Teh SJ (2008) Effect of dietary methylmercury and selenomethionine on Sacramento splittail larvae. Sci Total Environ 407:197-203. https://doi.org/10.1016/j.scitotenv.2008.08.028
  10. Gholami-Seyedkolaei SJ, Mirvaghefi A, Farahmand H, Kosari AA, Gholami-Seyedkolaei SJ, Gholami-Seyedkolaei SJ (2013) Optimization of recovery patterns in common carp exposed to roundup using resonse surface methodology: Evaluation of neurotoxicity and genotoxicity effects and biochemical parameters. Ecotox Environ Saf 98:152-161. https://doi.org/10.1016/j.ecoenv.2013.09.009
  11. Harper HA, Rodwell VW, Mayer PA (1978) Review of Physiological Chemistry, 17th ed. Lange Medical Publication, California.
  12. Jensen FB, Lecklin T, Busk M, Bury NR, Wilson RW, Wood CM, Grosell M (2002) Physiological impact of salinity increase at organism and red blood cell levels in the European flounder (Platichthys flesus). J Exp Mar Biol Ecol 247:159-174.
  13. Kindle KR, Whitmore DH (1986) Biochemical indicators of thermal stress in Tilapia aurea (Steindachner). J Fish Biol 29:243-255. https://doi.org/10.1111/j.1095-8649.1986.tb04942.x
  14. Krome CA (2014) Studies on the suitability of Jatropha curcas kernel meal as an alternative protein source in diets for carp (Cyprinus carpio) and tilapia (Oreochromis niloticus). Thesis. University of Stirling, Stirling, UK.
  15. Laiz-Carrion R, Guerreiro PM, Fuentes J, Canario AVM, Martin del Rio MP, Mancera JM (2005) Branchial osmoregulatory response to salinity in the gilthead sea bream, Sparus auratus. J Exp Zool 303:563-576.
  16. Laiz-Carrion R, Sangiao-Alvarellos S, Guzman JM, Martin del Rio MP, Minguez J, Soengas JL, Mancera JM (2002) Energy metabolism in fish tissues related to osmoregulation and cortisol action. Fish Physiol Biochem 27:179-188. https://doi.org/10.1023/B:FISH.0000032725.96481.b8
  17. Lee JW, Kim JW, De Riu N, Moniello G, Hung SSO (2012) Histopathological alterations of juvenile green (Acipenser medirostris) and white sturgeon (Acipenser transmontanus) exposed to graded levels of dietary methylmercury. Aquat Toxicol 109:90-99. https://doi.org/10.1016/j.aquatox.2011.12.008
  18. Lu W, Li W, Ke C, Wang H (2015) Reproductive success under the joint influences of temperature and salinity in noble scallop, Chlamys nobilis (Reeve). Aquacult Res, 1-11.
  19. Ming J, Xie J, Wu P, Ge X, Liu W, Ye J (2012) Effects of emodin and vitamin C on growth performance, biochemical parameters and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala Yih) under high temperature stress. Fish Shellfish Immun 32:651-661. https://doi.org/10.1016/j.fsi.2012.01.008
  20. Mommsen TP, Vijayan MM, Moon TW (1999) Cortisol in teleost: Dynamics, mechanisms of action, and metabolic regulation. Rev Fish Biol Fish 9:211-268. https://doi.org/10.1023/A:1008924418720
  21. Morris AV, Roberts C, Hawkins J (2000) The threatened status of groupers (Epinephelinae). Biodivers Conserv 9:919-942. https://doi.org/10.1023/A:1008996002822
  22. Moyle PB, Cech JJ (2004) An Introduction to Ichthyology. Fifth ed. Prentice Hall, Upper Saddle River, NJ, USA
  23. Park, JY, Cho, JK, Son, MH, Kim, KM, Han, KH, Park, JM (2016) Artificial spawning behavior and development of eggs, larvae and juveniles of the red spotted grouper, Epinephelus akaara in Korea. Dev Reprod 20:31-40.
  24. Randall JE (1987) A preliminary synopsis of the groupers (Perciformes: Serranidae: Epinephelinae) of the Indo-Pacific region. In: Polovina JJ and Ralston S (ed.), Tropical Snappers and Groupers: Biology and Fisheries Management. Westview Press, Boulder, Colorado, USA, pp 89-189.
  25. Rodwell VW (1988) Metabolism of proteins and amino acids. In: Rodwell VW (ed.), Review of Biochemistry. Lange Medical Publications, California, USA, pp 265-319.
  26. Sangiao-Alvarellos S, Arjona FJ, Martin del Rio MP, Miguez JM, Mancera JM, Soengas JL (2005) Time course of osmoregulatory and metabolic changes during osmotic acclimation in Sparus auratus. J Exp Biol 208:4291-4304. https://doi.org/10.1242/jeb.01900
  27. Shahkar E, Kim D-J, Mohseni M, Yun H, Bai SC (2015) Effects of salinity changes on hematological responses in juvenile ship sturgeon Acipenser nudiventris. Fish Aquat Sci 18:45-50.
  28. Teh SJ, Adams SM, Hinton DE (1997) Histopathologic biomarkers in feral freshwater fish populations exposed to different types of contaminant stress. Aquat Toxicol 37:51-70. https://doi.org/10.1016/S0166-445X(96)00808-9
  29. Teh SJ, Deng X, Deng DF, Teh FC, Hung SSO (2004) Chronic effects of dietary selenium on juvenile Sacramento splittail (Pogonichthys macrolepidotus). Environ Sci Technol 38:6085-6093. https://doi.org/10.1021/es049545+
  30. Vargas-Chacoff L, Arjona FJ, Polakof S, Martin del Rio MP, Soengas JL, Mancera JM (2009) Interactive effects of environmental salinity and temperature on metabolic responses of gilthead sea bream Sparus aurata. Comp Biochem Physiol Part A 154:417-424. https://doi.org/10.1016/j.cbpa.2009.07.015
  31. Wang YL, Xiong K, Yang J, Wu Z, Sheng XM, Tang HF (2005) Effect of beta-cypermethrin on GPT and GOT activities of crucian serum. Agr Sci Technol 6:20-23.