References
- Altman JD, Moss PA, Goulder PJ, Barouch DH, McHeyzerWilliams MG, Bell JI, et al. 1996. Phenotypic analysis of antigen-specific T lymphocytes. Science 274: 94-96. https://doi.org/10.1126/science.274.5284.94
- Bryksin AV, Matsumura I. 2010. Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids. Biotechniques 48: 463-465. https://doi.org/10.2144/000113418
- Chen GJ, Qiu N, Karrer C, Caspers P, Page MG. 2000. Restriction site-free insertion of PCR products directionally into vectors. Biotechniques 28: 498-500.
-
Grozdanov PN, MacDonald CC. 2015. Generation of plasmid vectors expressing FLAG-tagged proteins under the regulation of human elongation factor-1
${\alpha}$ promoter using Gibson assembly. J. Vis. Exp. 96: e52235. - Hansen T, Yu YY, Fremont DH. 2009. Current Protocols in Immunology, pp. 17.5.1-17.5.17. John Wiley & Sons, Inc. New Jersey.
- Heckman KL, Pease LR. 2007. Gene splicing and mutagenesis by PCR-driven overlap extension. Nat. Protoc. 2: 924-932. https://doi.org/10.1038/nprot.2007.132
- Luo WG, Liu HZ, Lin WH, Kabir MH, Su Y. 2013. Simultaneous splicing of multiple DNA fragments in one PCR reaction. Biol. Proced. Online 15: 1-9. https://doi.org/10.1186/1480-9222-15-1
- Meng F, Shen C, He Y, Miao F, Zhang J. 2009. Cloning and expression of three peptide-linked beta2-microglobulin molecules in Escherichia coli with an isocaudamer technique. Biotechnol. Lett. 31: 831-836. https://doi.org/10.1007/s10529-009-9950-3
- Mitaksov V, Truscott SM, Lybarger L, Connolly JM, Hansen TH, Fremont DH. 2007. Structural engineering of pMHC reagents for T cell vaccines and diagnostics. Chem. Biol. 14: 909-922. https://doi.org/10.1016/j.chembiol.2007.07.010
- Reddy TR, Fevat LM, Munson SE, Stewart AF, Cowley SM. 2015. Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coli. PLoS One 10: e0120681. https://doi.org/10.1371/journal.pone.0120681
- Shao H, Fan Z, Wan Z, Tian X, Chen H, Perez DR, et al. 2015. An efficient and rapid influenza gene cloning strategy for reverse genetics system. J. Virol. Methods 222: 91-94. https://doi.org/10.1016/j.jviromet.2015.06.001
- Shen C, He Y, Meng F, Cheng K, Zhang D, Miao F, Zhang J. 2011. Characterization of MHC/peptide complexes refolded by a one-step ion-exchange chromatography. J. Immunol. Methods 369: 81-90. https://doi.org/10.1016/j.jim.2011.04.014
- Spiliotis M. 2012. Inverse fusion PCR cloning. PLoS One 7: e35407. https://doi.org/10.1371/journal.pone.0035407
- Van den Ent F, Löwe J. 2006. RF cloning: a restriction-free method for inserting target genes into plasmids. J. Biochem. Biophys. Methods 67: 67-74. https://doi.org/10.1016/j.jbbm.2005.12.008
- Yang JK, Chen FY, Yan XX, Miao LH, Dai JH. 2012. A simple and accurate two-step long DNA sequences synthesis strategy to improve heterologous gene expression in Pichia. PLoS One 7: e36607. https://doi.org/10.1371/journal.pone.0036607
- Yu YY, Netuschil N, Lybarger L, Connolly JM, Hansen TH. 2002. Cutting edge: single-chain trimers of MHC class I molecules form stable structures that potently stimulate antigen-specific T cells and B cells. J. Immunol. 168: 3145-3149. https://doi.org/10.4049/jimmunol.168.7.3145
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