Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Components from Glycosmis stenocarpa on Pepper Mild Mottle Virus

  • Kim, Jang Hoon (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoon, Ju-Yeon (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kwon, Sun Jung (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA) ;
  • Cho, In Sook (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA) ;
  • Nguyen, Manh Cuong (Department of Bioactive Products, Vietnam Academy of Science and Technology) ;
  • Choi, Seung-Kook (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kim, Young Ho (College of Pharmacy, Chungnam National University) ;
  • Choi, Gug Seoun (Department of Horticultural and Crop Environment, National Institute of Horticultural and Herbal Science, RDA)
  • Received : 2016.07.14
  • Accepted : 2016.08.26
  • Published : 2016.12.28
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Abstract

The goal of this study was to identify a source of natural plant compounds with inhibitory activity against pepper mild mottle virus (PMMoV). We showed, using a half-leaf assay, that murrayafoline-A (1) and isomahanine (2) isolated from the aerial parts of Glycosmis stenocarpa have inhibitory activity against PMMoV through curative, inactivation, and protection effects. Using a leaf-disk assay, we confirmed that 2 inhibited virus replication in Nicotiana benthamiana. Using electron microscopy, we found that a mixture of the virus with 2 resulted in damage to the rod-shaped virus.

Keywords

References

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