Asian-Australasian Journal of Animal Sciences

  • 제29권1호
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  • Pages.134-141
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  • 2016
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effect of Polysaccharides from Acanthopanax senticosus on Intestinal Mucosal Barrier of Escherichia coli Lipopolysaccharide Challenged Mice

  • Han, Jie (Institute of Animal Husbandry and Veterinary, Shenyang Agricultural University) ;
  • Xu, Yunhe (College of Animal Husbandry and Veterinary, Liaoning Medical University) ;
  • Yang, Di (Institute of Animal Husbandry and Veterinary, Shenyang Agricultural University) ;
  • Yu, Ning (Institute of Biotechnology Research, Liaoning Academy of Agricultural Sciences) ;
  • Bai, Zishan (Institute of Animal Husbandry and Veterinary, Shenyang Agricultural University) ;
  • Bian, Lianquan (Institute of Animal Husbandry and Veterinary, Shenyang Agricultural University)
  • 투고 : 2015.06.24
  • 심사 : 2015.08.16
  • 발행 : 2016.01.01
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초록

To investigate the role of polysaccharide from Acanthopanax senticosus (ASPS) in preventing lipopolysaccharide (LPS)-induced intestinal injury, 18 mice (at 5 wk of age) were assigned to three groups with 6 replicates of one mouse each. Mice were administrated by oral gavage with or without ASPS (300 mg/kg body weight) for 14 days and were injected with saline or LPS at 15 days. Intestinal samples were collected at 4 h post-challenge. The results showed that ASPS ameliorated LPS-induced deterioration of digestive ability of LPS-challenged mice, indicated by an increase in intestinal lactase activity (45%, p<0.05), and the intestinal morphology, as proved by improved villus height (20.84%, p<0.05) and villus height:crypt depth ratio (42%, p<0.05), and lower crypt depth in jejunum (15.55%, p<0.05), as well as enhanced intestinal tight junction proteins expression involving occludin-1 (71.43%, p<0.05). ASPS also prevented intestinal inflammation response, supported by decrease in intestinal inflammatory mediators including tumor necrosis factor ${\alpha}$ (22.28%, p<0.05) and heat shock protein (HSP70) (77.42%, p<0.05). In addition, intestinal mucus layers were also improved by ASPS, as indicated by the increase in number of goblet cells (24.89%, p<0.05) and intestinal trefoil peptide (17.75%, p<0.05). Finally, ASPS facilitated mRNA expression of epidermal growth factor (100%, p<0.05) and its receptor (200%, p<0.05) gene. These results indicate that ASPS can prevent intestinal mucosal barrier injury under inflammatory conditions, which may be associated with up-regulating gene mRNA expression of epidermal growth factor and its receptor.

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참고문헌

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