KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Anthocyanin Analysis of Pressure-extracted Korean Blueberry Juice and in vitro Anti-inflammatory in RAW267.4 Cell line

국산 블루베리 착즙액의 안토시아닌 분석 및 RAW267.4 세포주에서의 항염효과

  • Choi, Moon-Hee (Major in Cosmetic Engineering, Department of Chemical Engineering, Graduate School of Engineering, Chosun University) ;
  • Jeon, Young-Jin (Department of Pharmacology, Chosun University) ;
  • Shin, Hyun-Jae (Major in Cosmetic Engineering, Department of Chemical Engineering, Graduate School of Engineering, Chosun University)
  • 최문희 (조선대학교 대학원 화학공학과 향장공학) ;
  • 전영진 (조선대학교 의과대학, 약리학) ;
  • 신현재 (조선대학교 대학원 화학공학과 향장공학)
  • Received : 2015.06.15
  • Accepted : 2015.08.15
  • Published : 2015.08.27
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Abstract

Blueberry juice possesses rich-procyanidins and - anthocyanidin, comprised a group of with numerous health benefits such as protection against coronary heart disease, detoxification, and obesity. Blueberry (Vaccinium virgatum) juice extracts were analyzed and separated by an HPLC method for the purpose of the separation and quantification in polyphenolic groups. In specific HPLC conditions, a binary mobile phase consisting of formic acid: water (10:90, v/v, solvent A) and formic acid: water: acetonitrile (10:60:30, v/v/v, solvent B) was utilized and it is detected at 546 nm wavelength. The phenolic contents of the extracts are determined using Folin-Ciocalteu phenol reagent. In order to test anti-inflammation activity assay, after producing nitric oxide (NO) in lipopolysaccharide activated RAW 264.7 cells, at concentration of $20-500{\mu}g/mL$ it reduced to NO production at a dose-dependent manner. Importantly, cytotoxicity assay with up to $500{\mu}g/mL$ of the extract from blueberry juice showed ~100% cell viability for RAW264.7 cell line. Therefore, Korean blueberry juice might have potential as anti-oxidant and antiinflammation agents.

Keywords

References

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