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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Development of a Validated Determination of Methylsulfonylmethane in Dietary Supplement by Gas Chromatography

기체크로마토그래피를 이용한 식이보충제에서 메틸설포닐메탄의 검증된 분석법 개발

  • Park, Sang-Wook (Hazardous Substances Analysis Division, Gwangju Regional Food & Drug Administration) ;
  • Lee, Wonjae (College of Pharmacy, Chosun University)
  • 박상욱 (광주지방식품의약품안전청 유해물질분석과) ;
  • 이원재 (조선대학교 약학대학 약학과)
  • Received : 2015.04.30
  • Accepted : 2015.07.27
  • Published : 2015.08.27
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Abstract

The convenient determination of methylsulfonylmethane (MSM) for a commercially available dietary supplement was developed using gas chromatography (GC)-flame ionization detector (FID). Chromatography was performed on a capillary column ($0.32mm\;I.D{\times}30m$, $0.25{\mu}m$) coated with dimethylpolysiloxane using diethylene glycol methyl ether as an internal standard. The performance characteristics of GC were evaluated in terms of selectivity, linearity, precision, accuracy, recovery, limit of detection (LOD) and limit of quantification (LOQ). The calibration curve was highly linear (the coefficient of determination: 0.9979) within the concentration range of $10.0{\sim}800.0{\mu}g/mL$ for MSM. The recoveries for three fortified concentrations were 96.7~97.1%, 96.6~97.3% and 96.8~97.2%, respectively. The LOD and LOQ of the method were $0.29{\mu}g/mL$ and $0.97{\mu}g/mL$, respectively. All obtained results were acceptable according to the guidelines of the Association of Official Analytical Chemists for dietary supplements. Thus, the validated analytical method using the GC-FID system is suitable for the determination of MSM in dietary supplement formulations for quality control.

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References

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