Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Fermented Water Extracts from Ligularia fischeri on Hepatotoxicity Induced by D-Galactosamine in Rats

D-Galactosamine 투여 랫트에서 곰취 열수 추출 발효물이 간 독성 저하에 미치는 영향

  • Yu, Keun-Hyung (Department of Technical Development Chuncheon Bioindustry Foundation) ;
  • Lee, Sun-Yeop (Department of Technical Development Chuncheon Bioindustry Foundation) ;
  • Yang, Hyun-Mo (Department of Technical Development Chuncheon Bioindustry Foundation) ;
  • Ham, Young-Ahn (Department of Technical Development Chuncheon Bioindustry Foundation) ;
  • Lee, Soo-Ung (Department of Technical Development Chuncheon Bioindustry Foundation) ;
  • Chae, Seoung-Wan (Department of Pathology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine) ;
  • Lee, Yong-Jin (Department of Technical Development Chuncheon Bioindustry Foundation)
  • 유근형 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 이선엽 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 양현모 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 함영안 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 이수응 ((재)춘천바이오산업진흥원 기술개발실) ;
  • 채승완 (성균관대학교 의과대학 강북삼성병원 병리과) ;
  • 이용진 ((재)춘천바이오산업진흥원 기술개발실)
  • Received : 2015.06.19
  • Accepted : 2015.08.10
  • Published : 2015.10.31
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Abstract

This study was conducted to determine the effect of fermented water extracts from Ligularia fischeri (LAF) on reduction of hepatotoxicity induced by D-galactosamine (D-GalN) in rats. In this experiment, male Sprague-Dawley rats were used as experimental animals, which were divided into eight groups: normal group, D-GalN-treated group (control), D-GalN and non-fermented water extracts from Ligularia fischeri (LA)-treated groups [100, 200, and 400 mg/kg BW (body weight)], and D-GalN and LAF-treated groups (100, 200, and 400 mg/kg BW). ${\gamma}$-Glutamyl transferase, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase activities in serum of the D-GalN and LAF-treated groups decreased significantly compared to those of the control group (P<0.05). The high density lipoprotein-cholesterol levels of the D-GalN and LAF-treated groups increased significantly compared to those of the control group (P<0.05). The low density lipoprotein-cholesterol and triglyceride levels of the D-GalN and LAF-treated groups decreased significantly compared to those of the control group (P<0.05). The atherogenic index values of the D-GalN and LAF-treated groups decreased significantly compared to those of the control group (P<0.05), and their high density lipoprotein cholesterol by total cholesterol ratio increased significantly in these groups (P<0.05). Superoxide dismutase activity of liver tissues were enhanced significantly (P<0.05) in the D-GalN and LAF-treated groups compared to that of the control group (P<0.05), whereas their malondialdehyde content decreased significantly in these groups (P<0.05). The histopathological observations revealed apoptotic cells and mild portal inflammation in liver tissues of the D-GalN and LAF-treated groups. Taken together, these results demonstrate that LAF may improve plasma lipid profile and alleviate hepatic damage.

본 연구는 산채류 중 널리 섭취되고 있는 곰취의 활용가치를 높이고 건강기능식품 소재로써의 가능성을 확인고자 DGalN 동물모델을 통해 곰취 열수 추출 발효물의 간 기능 개선 효과를 연구하였다. 실험기간 동안 실험동물의 체중 증가량은 처리군 간 유의적인 차이가 없었으며(P>0.05), 간 무게는 곰취 열수 추출물 투여군에서 유의하게 증가하는 것으로 나타났다(P<0.05). 혈청 중 GGT 분석 결과 곰취 열수 추출물과 발효물 투여군에서 대조군과 비교하여 유의하게 감소하였다(P<0.05). 특히 발효물 투여군의 경우 GGT, AST, ALT 및 LDH의 효소 활성이 대조군과 비교하여 40% 이상 감소하였다(P<0.05). 총 콜레스테롤의 함량을 측정한 결과 정상식이군과 비교하여 모든 투여군에서 유의하게 감소하였으며(P<0.05), HDL-콜레스테롤의 경우 발효물 투여군에서 대조군과 비교하여 유의하게 증가하였다(P<0.05). LDL-콜레스테롤은 농도 의존적으로 유의하게 감소하였다(P<0.05). 중성지방의 함량은 발효물 투여군에서 대조군과 비교하여 유의하게 감소하였으며(P<0.05), 총 콜레스테롤 함량 중 HDL-콜레스테롤 함량의 비율은 발효물 투여군에서 대조군과 비교하여 유의하게 증가하는 것으로 나타났고(P<0.05), 동맥경화지수의 경우 발효물 투여군에서 대조군과 비교하여 유의하게 감소하였다(P<0.05). 발효물의 경우 SOD 활성이 유의하게 증가하였으며(P<0.05), 간 조직 내 과산화지질의 분해산물인 MDA 함량은 곰취 열수 추출물 및 발효물 투여군에서 대조군과 비교하여 MDA 함량이 유의하게 감소하였다(P<0.05). 간 조직 병리학적 분석 결과 곰취 열수 추출물 및 발효물 투여군에서 대조군과 비교하여 세포사멸사 지수, 문맥엽 염증 지수 등이 개선되는 경향을 보였으나 유의적인 차이는 없는 것으로 나타났다(P>0.05). 이상의 결과로 보아 곰취 열수 추출 발효물이 간 기능 효소의 활성 억제, 혈청지질 대사 개선, 간 조직 내 SOD 활성증가 및 과산화지질을 감소시켜 D-GalN으로 인한 간 손상을 완화시키는 데 효과적일 것으로 사료되며, 향후 건강기능 식품 소재로 이용 가능할 것으로 판단된다.

Keywords

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