Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Peptide H reduces Il-6 expression in human breast cancer MDA-MB-231 cells

인간유방암 MDA-MB-231세포에서 peptide H에 의한 TNFα 발현 억제

  • Sung, Dae Il (Department of Biotechnology, The Research Institute for Basic Sciences, Hoseo University) ;
  • Park, Jameon (Department of Biotechnology, The Research Institute for Basic Sciences, Hoseo University) ;
  • Kang, Choong Kyung (Department of Nanobiotronics, Bioconvergence Lab, Hoseo University) ;
  • Kim, Han Bok (Department of Biotechnology, The Research Institute for Basic Sciences, Hoseo University)
  • 성대일 (호서대학교 생명공학과 기초과학연구소) ;
  • 박잠언 (호서대학교 생명공학과 기초과학연구소) ;
  • 강충경 (호서대학교 나노바이오트로닉스학과 바이오융합연구소) ;
  • 김한복 (호서대학교 생명공학과 기초과학연구소)
  • Received : 2015.07.28
  • Accepted : 2015.09.12
  • Published : 2015.09.30
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Abstract

Chungkookjang (fermented soybeans) contains diverse peptides. Human breast cancer MDA-MB-231 cells were treated with peptide H derived from Chungkookjang, and $TNF{\alpha}$ expression in the cells was conspicuously repressed, suggesting peptide H's regulation of IL6 since IL6 is induced by $TNF{\alpha}$. The structure of peptide H was different from those of glucocorticoid and dexamethasone, suggesting different mechanisms of $TNF{\alpha}$ expression suppression. Peptide H which reduces $TNF{\alpha}$ expression can be developed as drugs for cancer, rheumatoid arthritis and Crohn's disease, after more investigation.

청국장은 다양한 peptide류를 포함한다. 청국장 유래의 peptide H를 인간유방암 MDA-MB-231 세포에 처리했을 때, $TNF{\alpha}$ 발현은 뚜렷하게 억제되었다. $TNF{\alpha}$에 의해 유도되는 IL6 발현 역시, peptide H에 의해 감소될 수 있음을 시사해 준다. Peptide H구조는 glucocorticoid, dexamethasone과 전혀 유사하지 않아 그들과 다른 기작으로 $TNF{\alpha}$ 발현억제에 작용할 것을 시사해 준다. Peptide H는 $TNF{\alpha}$ 발현 억제 효과가 있으므로 보다 깊이 있는 연구를 바탕으로, 류마티스 관절염, 크론병 등의 치료제로 개발될 수 있기를 기대해 본다.

Keywords

References

  1. Berman, H.M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T.N., Weissig, H., Shindyalov, I.N., and Bourne, P.E. 2000. The protein data bank. Nucleic Acids Res. 28, 235-242. https://doi.org/10.1093/nar/28.1.235
  2. Hwang, J.S., Yoo, H.J., Song, H.J., Kim, K.K., Chun, Y.J., Matsui, T., and Kim, H.B. 2011. Inflammation-related signaling pathways implicating $TGF{\beta}$ are revealed in the expression profiling of MCF7 cell treated with fermented soybean, Chungkookjang. Nutr. Cancer 63, 645-652. https://doi.org/10.1080/01635581.2011.551987
  3. Landskron, G., De la Fuente, M., Thuwajit, P., Thuwajit, C., and Hermoso, M.A. 2014. Chronic inflammation and cytokines in the tumor microenvironment. J. Immunol. Res. http://dx.doi.org/10.1155/2014/149185.
  4. Lee, J.J., Lee, D.S., and Kim, H.B. 1999. Fermentation patterns of Chungkookjang and Kanjang by Bacillus licheniformis B1. Kor. J. Microbiol. 35, 296-301.
  5. Lee, W.H., Wu, H.M., Lee, C.G., Sung, D.I., Song, H.J., Matsui, T., Kim, H.B., and Kim, S.G. 2014. Specific oligopeptides in fermented soybean extract inhibit NF-${\kappa}B$-dependent iNOS and cytokine induction by toll like receptor ligands. J. Med. Food 17, 1239-1246. https://doi.org/10.1089/jmf.2013.3070
  6. Lin, H., Lin, T., Wu, M., Chiu, Y., Tang, C., Hour, M., Liou, H., Tu, H., Yang, R., and Fu, W. 2014. 5-Lipoxigenase inhibitors attenuate TNF-${\alpha}$-induced inflammation in human synovial fibroblasts. PLoS One 9, e107890. https://doi.org/10.1371/journal.pone.0107890
  7. Matsui, T., Yoo, H.J., Hwang, J.S., Lee, D.S., and Kim, H.B. 2004. Isolation of angiotensin-1-converting enzyme inhibitory peptide from Chungkookjang. Kor. J. Microbiol. 40, 355-358.
  8. McInners, I.B., Leung, B.P., Sturock, R.D., Field, M., and Liew, F.Y. 1997. Interleukin-15 mediates T cell-dependent regulation of tumor necrosis factor-${\alpha}$ production in rheumatoid arthritis. Nature Med. 3, 189-195. https://doi.org/10.1038/nm0297-189
  9. Ndlovu, M.N., Lint, C.V., Wesmael, K.V., Callebert, P., Chalbos, D., Haegeman, G., and Berghe, W.V. 2009. Hyperactivated NF-${\kappa}B$ and AP-1 transcription factors promote highly accessible chromatin and constitutive transcription across the interleukin-6 gene promoter in metastatic breast cancer cells. Mol. Cell Biol. 29, 5488-5504. https://doi.org/10.1128/MCB.01657-08
  10. Sawada, M., Suzumura, A., and Marunouchi, T. 1992. TNF${\alpha}$ induces IL-6 production by astrocytes but not by microglia. Brain Res. 583, 296-299. https://doi.org/10.1016/S0006-8993(10)80037-X
  11. Somvanshi, R.K., Singh, A.K., Saxena, M., Mishra, B., and Dey, S. 2008. Development of novel peptide inhibitor of lipoxygenase based on biochemical and BlAcore evidences. Biochim. Biophys. Acta 1784, 1812-1817. https://doi.org/10.1016/j.bbapap.2008.07.004
  12. Sung, D.I., Park, J., and Kim, H.B. 2014. Peptide H reduces IL-6 expression in human breast cancer MDA-MB-231 cells. Kor. J. Microbiol. 50, 261-263. https://doi.org/10.7845/kjm.2014.4048
  13. Swantek, J.L., Cobb, M.H., and Geppert, T.D. 1997. Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) is required for lipopolysaccharide stimulation of tumor necrosis factor alpha (TNF${\alpha}$) translation: glucocorticoids inhibit TNF${\alpha}$translation by blocking JNK/SAPK. Mol. Cell. Biol. 17, 6274-6282. https://doi.org/10.1128/MCB.17.11.6274
  14. Tanabe, K., Matsushima-Nishiwaki, R., Yamaguchi S., Iida, H., Dohi, S., and Kozawa, O. 2010. Mecanisms of tumor necrosis factor-alpha-induced interleukin-6 synthesis in glioma cells. J. Neuroinflam. 7, 16. https://doi.org/10.1186/1742-2094-7-16
  15. Trabuco, L.G., Lise, S., Petsalaki, E., and Russell, R.B. 2012. PepSite: prediction of peptide-binding sites from protein surfaces. Nucleic Acids Res. 40, 423-427. https://doi.org/10.1093/nar/gks398
  16. Van Dullemen, H.M., Deventer, S.J.H., Homes, D.W., Bijl, H.A., Jansen, J., Tytgat, G.N.J., and Woody, J. 1995. Treatment of Crohn's disease with anti-tumer neucrosis factor chimeric monoclonal antibody (cA2). Gastroenterol. 109, 129-135. https://doi.org/10.1016/0016-5085(95)90277-5
  17. Wenzel, M., Chiriac, A.L., Otto, A., zweytick, D., May, C., Schumacher, C., Gust, R., Albada, B., Penkova, M., Kramer U., et al. 2014. Small cationic antimicrobial peptides delocalize peripheral membrane proteins. Proc. Natl. Acad. Sci. USA 111, 1409-1418. https://doi.org/10.1073/pnas.1323697111

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