Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Melanogenesis Inhibitory Activities of Mulberry Seed Ethanol Extracts

오디씨 에탄올 추출물의 멜라닌 합성 억제효과

  • Jeong, Yong Tae (HK bio, business incubator, Daegu Haany University) ;
  • Kang, Min Ju (HK bio, business incubator, Daegu Haany University) ;
  • Kim, Jin Hee (College of herbal Bio-Industry, Daegu Haany University)
  • 정용태 (에이치케이(HK)바이오) ;
  • 강민주 (에이치케이(HK)바이오) ;
  • 김진희 (대구한의대학교 한방산업대학)
  • Received : 2015.06.19
  • Accepted : 2015.09.14
  • Published : 2015.09.30
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Abstract

The purpose of this study was to investigate anti-melanogenesis effects of mulberry seed extracts (MSE). MSE inhibited melanogenesis in melan-a cells at $10{\mu}g/mL$ without cytotoxicity. Also, MSE decreased tyrosinase, tyrosinase-related protein-1 (TRP-1) protein expression in the melan-a cells. To identify the signaling pathway of MSE, the ability of MSE to influence extracellular signal-regulated protein kinase (ERK) activation was investigated. MSE induced ERK protein expression in a dose-dependent manner. In addition, MSE presented inhibition of the body pigmentation in vivo zebrafish model. These results suggest that MSE may be an effective anti-melanogenesis agent regulating the expression of ERK protein and melanogenic enzymes.

본 연구는 오디씨 에탄올 추출물(MSE)의 멜라닌 합성 저해 효과를 밝히는 것이다. 먼저 MSE의 melan-a 세포를 이용한 멜라닌 합성 저해 실험결과, 독성을 보이지 않는 $10{\mu}g/mL$의 농도까지 멜라닌 합성 저해 효과를 나타내었다. 또한 tyrosinase, tyrosinase-related protein-1 (TRP-1) 단백질의 발현이 저해되었으며, extracelluar signal-regulated protein kinase (ERK)의 발현을 농도 의존적으로 증가시키는 MSE의 기전을 확인하였다. 뿐만 아니라, 제브라피쉬를 이용한 in vivo 모델의 실험에서도 색소 발생이 저해됨을 관찰하였다. 따라서 오디씨로 부터 획득한 에탄올 추출물이 ERK 단백질의 발현으로 인해 멜라닌 생합성을 억제할 수 있음을 밝혔다.

Keywords

References

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