농약과학회지 (The Korean Journal of Pesticide Science)

  • 제19권3호
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  • Pages.230-240
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  • 2015
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지
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농작업자의 Kresoxim-methyl과 fenthion에 대한 호흡노출량 측정을 위한 IOM 채집기의 효율성 평가

Evaluation for Application of IOM Sampler for Agricultural Farmer's Inhalation Exposure to Kresoxim-methyl and Fenthion

  • 이지호 (서울대학교 농생명공학부) ;
  • 김은혜 (서울대학교 농생명공학부) ;
  • 이종화 (서울대학교 농생명공학부) ;
  • 신용호 (서울대학교 농생명공학부) ;
  • ;
  • 최훈 (원광대학교 식품.환경학부) ;
  • 문준관 (한경대학교 식물생명환경과학과) ;
  • 이혜리 (서울대학교 농생명공학부) ;
  • 김정한 (서울대학교 농생명공학부)
  • Lee, Jiho (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Eunhye (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Jonghwa (Department of Agricultural Biotechnology, Seoul National University) ;
  • Shin, Yongho (Department of Agricultural Biotechnology, Seoul National University) ;
  • Maasfeld, Wolfgang (European Crop Protection Association (ECPA)) ;
  • Choi, Hoon (Division of Food and Environmental Sciences, Wonkwang University) ;
  • Moon, Joon-Kwan (Department of Plant Life and Environmental Sciences, Hankyong National University) ;
  • Lee, Hyeri (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jeong-Han (Department of Agricultural Biotechnology, Seoul National University)
  • 투고 : 2015.08.21
  • 심사 : 2015.09.17
  • 발행 : 2015.09.30
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초록

본 연구는 농작업자의 호흡노출 측정에 사용되어오던 고체흡착제 외에 최근에 사용되는 유리섬유여과지가 장착된 IOM 채집기에 대해서, 고체 제제인 kresoxim-methyl 입상수화제와 액상 제제인 fenthion 유제를 이용하여 포집효율과 파과율을 측정하여 효율성을 검증하고자 하였다. LC-MS/MS 기기상 최소검출량은 12.5 pg, 분석법상 검출한계는 5.0 ng/mL이었고, 각 노출 시료의 matrix matched standard의 직선성은 $R^2$ 값이 0.999 이상이었다. 사용된 유리섬유여과지와 고체흡착제에서의 두 가지 농약의 회수율은 유리섬유여과지는 kresoxim-methyl 102-109%, fenthion 97-104%, XAD-2 resin 고체흡착제는 각각 94-98%, 93-100%이었다. 포집효율은 IOM 채집기(유리섬유여과지 장착)와 고체흡착제(XAD-2 resin)를 연결한 후 개인용 공기펌프에 연결하여 측정하였다. 고체 및 액상 2가지 제제를 표준배율로 희석하여 IOM 채집기에 분무하여 포집효율을 본 결과 두 제제 모두 유리섬유여과지에 포집되었고 고체흡착제로 통과되지 않았다. 파과시험은 IOM 채집기의 유리섬유여과지에 농약 표준 용액을 가하고 고체흡착제를 연결한 후 개인용 공기펌프에 연결하여 측정하였다. 파과시험 결과, kresoxim-methyl은 87-101%, fenthion은 96-105%가 첫번째 유리섬유여과지에 흡착/보유되어 있었고 두 번째 유리섬유여과지나 고체흡착제로 파과되지 않았다. 따라서 유리섬유여과지가 장착된 IOM 채집기는 고체 제제나 액상 제제나 상관없이 포집효율과 흡착/보유 능력이 뛰어난 것으로 검증되어 농약 호흡노출 연구에 적극 활용될 것으로 판단한다.

An IOM sampler equipped with glass fiber filter has been recently utilized instead of solid adsorbent, which was used to measure the inhalation exposure of agricultural operator to pesticides. The aim of this study is to validate the efficacy of an IOM sampler by measuring the trapping efficiency and breakthrough using kresoxim-methyl water-dispersible granule and fenthion emulsifiable concentrate. On LC-MS/ MS, minimum detection level was 12.5 pg and method limit of detection was 5.0 ng/mL. Good linearity ($R^2$ > 0.999) for matrix matched standards was obtained. Recoveries of pesticides from glass fiber filter were 102-109% (kresoxim-methyl) and 97-104% (fenthion) while those from XAD-2 resin were 94-98% (kresoxim methyl) and 93-100% (fenthion). Trapping efficiency test was performed with personal air pumps and IOM sampler (glass fiber filter) connected with solid adsorbent (XAD-2 resin) with two types of formulation (solid and liquid) which were diluted by standard rate and sprayed to IOM sampler. Those pesticides were trapped only in glass fiber filter without any breakthrough to solid adsorbent. After spiking of pesticides to glass fiber filter, breakthrough test was carried out with IOM sampler (glass fiber filter) which was connected with solid adsorbent. As a results, 87-101% of kresoxim-methyl and 96-105% of fenthion remained in spiked glass fiber filter, however, no pesticides were detected in second glass fiber filter and solid adsorbent. In conclusion, IOM sampler which equipped with glass fiber filter can be applied widely for pesticide inhalation exposure study since it has good trapping efficiency and adsorption capacity, regardless of the solid or liquid formulation.

키워드

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  1. Validation protocol for whole-body dosimetry in an agricultural exposure study vol.61, pp.1, 2015, https://doi.org/10.1007/s13765-017-0330-8