DOI QR코드

DOI QR Code

Upregulation and Clinicopathological Significance of Long Non-coding NEAT1 RNA in NSCLC Tissues

  • Pan, Lin-Jiang (Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical University) ;
  • Zhong, Teng-Fei (Department of Pathology, First Affiliated Hospital of Guangxi Medical University) ;
  • Tang, Rui-Xue (Department of Pathology, First Affiliated Hospital of Guangxi Medical University) ;
  • Li, Ping (Department of Pathology, First Affiliated Hospital of Guangxi Medical University) ;
  • Dang, Yi-Wu (Department of Pathology, First Affiliated Hospital of Guangxi Medical University) ;
  • Huang, Su-Ning (Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical University) ;
  • Chen, Gang (Department of Pathology, First Affiliated Hospital of Guangxi Medical University)
  • 발행 : 2015.04.14

초록

Background: Recent reports have shown that nuclear enriched abundant transcript 1 (NEAT1), a long noncoding RNA (lncRNA), contributes to the precise control of gene expression and is related to several human malignancies. However, limited data are available on the expression and function of NEAT1 in lung cancer. The major objective of the current study was to profile the expression and clinicopathological significance of NEAT1 in non-small cell lung cancers (NSCLCs). Materials and Methods: NEAT1 expression in 125 NSCLC cases and paired adjacent non-cancer tissues was assessed by real-time quantitative reverse transcription-PCR (qRT-PCR). Relationships between NEAT1 and clinicopathological factors were also investigated. Results: The relative level of NEAT1 was $6.98{\pm}3.74$ in NSCLC tissues, significantly elevated as compared to that of the adjacent non-cancer lung tissues ($4.83{\pm}2.98$, p<0.001). The area under curve (AUC) of high expression of NEAT1 to diagnose NSCLC was 0.684 (95% CI: 0.619~0.750, p<0.001). NEAT1 expression was positively correlated with patient age (r=-2.007, p=0.047), lymphatic metastasis (r=-2.731, p=0.007), vascular invasion (r=-3.617, p=0.001) and clinical TNM stage (r=-4.134, p<0.001). Conclusions: This study indicates that NEAT1 might be associated with oncogenesis and progression in NSCLC, and suggests application in molecular targeted therapy.

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참고문헌

  1. Barrow TM, Michels KB (2014). Epigenetic epidemiology of cancer. Biochem Biophys Res Commun. 455, 70-83. https://doi.org/10.1016/j.bbrc.2014.08.002
  2. Brothers JF, Hijazi K, Mascaux C, et al (2013). Bridging the clinical gaps: genetic, epigenetic and transcriptomic biomarkers for the early detection of lung cancer in the post-National Lung Screening Trial era. BMC Med, 11, 168. https://doi.org/10.1186/1741-7015-11-168
  3. Cao J (2014). The functional role of long non-coding RNAs and epigenetics. Biol Proced Online, 16, 11. https://doi.org/10.1186/1480-9222-16-11
  4. Chen G, Kronenberger P, Teugels E, et al (2011). Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells. Biol Proced Online, 13, 1. https://doi.org/10.1186/1480-9222-13-1
  5. Chen G, Kronenberger P, Teugels E, et al (2012). Targeting the epidermal growth factor receptor in non-small cell lung cancer cells: the effect of combining RNA interference with tyrosine kinase inhibitors or cetuximab. BMC Med, 10, 28. https://doi.org/10.1186/1741-7015-10-28
  6. Chen G, Kronenberger P, Teugels E, et al (2013a). Effect of siRNAs targeting the EGFR T790M mutation in a non-small cell lung cancer cell line resistant to EGFR tyrosine kinase inhibitors and combination with various agents. Biochem Biophys Res Commun, 431, 623-9. https://doi.org/10.1016/j.bbrc.2012.12.070
  7. Chen G, Kronenberger P, Umelo IA, et al (2010). Quantification of epidermal growth factor receptor T790M mutant transcripts in lung cancer cells by real-time reverse transcriptase-quantitative polymerase chain reaction. Anal Biochem, 398, 266-8. https://doi.org/10.1016/j.ab.2009.11.034
  8. Chen G, Noor A, Kronenberger P, et al (2013b). Synergistic effect of afatinib with su11274 in non-small cell lung cancer cells resistant to gefitinib or erlotinib. PLoS One, 8, e59708. https://doi.org/10.1371/journal.pone.0059708
  9. Chen G, Umelo IA, Lv S, et al (2013c). miR-146a inhibits cell growth, cell migration and induces apoptosis in non-small cell lung cancer cells. PLoS One, 8, 60317. https://doi.org/10.1371/journal.pone.0060317
  10. Chen LL, Carmichael GG (2009). Altered nuclear retention of mRNAs containing inverted repeats in human embryonic stem cells: functional role of a nuclear noncoding RNA. Mol Cell, 35, 467-78. https://doi.org/10.1016/j.molcel.2009.06.027
  11. Chen X, Liu Y, Roe OD, et al (2013d). Gefitinib or erlotinib as maintenance therapy in patients with advanced stage non-small cell lung cancer: a systematic review. PLoS One, 8, e59314. https://doi.org/10.1371/journal.pone.0059314
  12. Dang YW, Zeng J, He RQ, et al (2014). Effects of miR-152 on cell growth inhibition, motility suppression and apoptosis induction in hepatocellular carcinoma cells. Asian Pac J Cancer Prev, 15, 4969-76. https://doi.org/10.7314/APJCP.2014.15.12.4969
  13. Gupta RA, Shah N, Wang KC, et al (2010). Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature, 464, 1071-6. https://doi.org/10.1038/nature08975
  14. Kim YS, Hwan JD, Bae S, et al (2010). Identification of differentially expressed genes using an annealing control primer system in stage III serous ovarian carcinoma. BMC Cancer, 10, 576. https://doi.org/10.1186/1471-2407-10-576
  15. Lee PN, Forey BA (2013). Indirectly estimated absolute lung cancer mortality rates by smoking status and histological type based on a systematic review. BMC Cancer, 13, 189. https://doi.org/10.1186/1471-2407-13-189
  16. Li G, Zhang H, Wan X, et al (2014). Long noncoding RNA plays a key role in metastasis and prognosis of hepatocellular carcinoma. Biomed Res Int, 2014, 780521.
  17. Li SG, Chen HY, Ou-Yang CS, et al (2013). The efficacy of Chinese herbal medicine as an adjunctive therapy for advanced non-small cell lung cancer: a systematic review and meta-analysis. PLoS One, 8, 57604. https://doi.org/10.1371/journal.pone.0057604
  18. Liu JH, Chen G, Dang YW, et al (2014). Expression and prognostic significance of lncRNA MALAT1 in pancreatic cancer tissues. Asian Pac J Cancer Prev, 15, 2971-7. https://doi.org/10.7314/APJCP.2014.15.7.2971
  19. Ma H, Hao Y, Dong X, et al (2012). Molecular mechanisms and function prediction of long noncoding RNA. ScientificWorld J, 2012, 541786.
  20. Naganuma T, Hirose T (2013). Paraspeckle formation during the biogenesis of long non-coding RNAs. RNA Biol, 10, 456-61. https://doi.org/10.4161/rna.23547
  21. Ramshankar V, Krishnamurthy A (2013). Lung cancer detection by screening - presenting circulating miRNAs as a promising next generation biomarker breakthrough. Asian Pac J Cancer Prev, 14, 2167-72. https://doi.org/10.7314/APJCP.2013.14.4.2167
  22. Rong M, He R, Dang Y, et al (2014). Expression and clinicopathological significance of miR-146a in hepatocellular carcinoma tissues. Ups J Med Sci, 119, 19-24. https://doi.org/10.3109/03009734.2013.856970
  23. Rosell R, Karachaliou N (2013). Lung cancer: Maintenance therapy and precision medicine in NSCLC. Nat Rev Clin Oncol, 10, 549-50. https://doi.org/10.1038/nrclinonc.2013.152
  24. Sen R, Ghosal S, Das S, et al (2014). Competing endogenous RNA: the key to posttranscriptional regulation. ScientificWorld J, 2014, 896206.
  25. Siegel R, Ma J, Zou Z, et al (2014). Cancer statistics. CA Cancer J Clin, 64, 9-29. https://doi.org/10.3322/caac.21208
  26. Tsai MC, Manor O, Wan Y, et al (2010). Long noncoding RNA as modular scaffold of histone modification complexes. Science, 329, 689-93. https://doi.org/10.1126/science.1192002
  27. Zeng C, Xu Y, Xu L, et al (2014). Inhibition of long non-coding RNA NEAT1 impairs myeloid differentiation in acute promyelocytic leukemia cells. BMC Cancer, 14, 693. https://doi.org/10.1186/1471-2407-14-693
  28. Zhao W, An Y, Liang Y, et al (2014). Role of HOTAIR long noncoding RNA in metastatic progression of lung cancer. Eur Rev Med Pharmacol Sci, 18, 1930-6.

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  1. Aberrant NEAT1 expression is associated with clinical outcome in high grade glioma patients vol.124, pp.3, 2015, https://doi.org/10.1111/apm.12480
  2. From junk to master regulators of invasion: lncRNA functions in migration, EMT and metastasis vol.139, pp.2, 2016, https://doi.org/10.1002/ijc.30039
  3. Enhanced expression of long non-coding RNA NEAT1 is associated with the progression of gastric adenocarcinomas vol.14, pp.1, 2016, https://doi.org/10.1186/s12957-016-0799-3
  4. Long noncoding RNA NEAT1 is an unfavorable prognostic factor and regulates migration and invasion in gastric cancer vol.142, pp.7, 2016, https://doi.org/10.1007/s00432-016-2152-1
  5. p53 induces formation of NEAT1 lncRNA-containing paraspeckles that modulate replication stress response and chemosensitivity vol.22, pp.8, 2016, https://doi.org/10.1038/nm.4135
  6. Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway vol.35, pp.1, 2016, https://doi.org/10.1186/s13046-016-0297-z
  7. The plasma lncRNA acting as fingerprint in non-small-cell lung cancer vol.37, pp.3, 2016, https://doi.org/10.1007/s13277-015-4023-9
  8. Long non-coding RNA NEAT1 decreases the chemosensitivity of gastric cancer cells via regulating P-glycoprotein expression vol.13, pp.3, 2017, https://doi.org/10.1007/s13273-017-0035-4
  9. Regulation of NEAT1/miR-214-3p on the growth, migration and invasion of endometrial carcinoma cells vol.295, pp.6, 2017, https://doi.org/10.1007/s00404-017-4365-1
  10. Long noncoding RNA TCF7 promotes invasiveness and self-renewal of human non-small cell lung cancer cells vol.30, pp.1, 2017, https://doi.org/10.1007/s13577-016-0147-5
  11. Clinical value of miR-145-5p in NSCLC and potential molecular mechanism exploration: A retrospective study based on GEO, qRT-PCR, and TCGA data vol.39, pp.3, 2017, https://doi.org/10.1177/1010428317691683
  12. NEAT1: A novel cancer-related long non-coding RNA vol.50, pp.2, 2017, https://doi.org/10.1111/cpr.12329
  13. Long non-coding RNA NEAT1 contributes to docetaxel resistance of prostate cancer through inducing RET expression by sponging miR-34a vol.7, pp.68, 2017, https://doi.org/10.1039/C7RA06107B
  14. Overexpression of lncRNA NEAT1 mitigates multidrug resistance by inhibiting ABCG2 in leukemia vol.12, pp.2, 2016, https://doi.org/10.3892/ol.2016.4738
  15. Long non-coding RNA NEAT1 regulates E2F3 expression by competitively binding to miR-377 in non-small cell lung cancer vol.14, pp.4, 2017, https://doi.org/10.3892/ol.2017.6769
  16. HIF-2α activated lncRNA NEAT1 promotes hepatocellular carcinoma cell invasion and metastasis by affecting the epithelial-mesenchymal transition vol.119, pp.4, 2018, https://doi.org/10.1002/jcb.26481
  17. Development of a pipeline for automated, high-throughput analysis of paraspeckle proteins reveals specific roles for importin α proteins vol.7, pp.1, 2017, https://doi.org/10.1038/srep43323
  18. Long non-coding RNA NEAT1 promotes hepatocellular carcinoma cell proliferation through the regulation of miR-129-5p-VCP-IκB vol.313, pp.2, 2017, https://doi.org/10.1152/ajpgi.00426.2016
  19. NEAT1_2 functions as a competing endogenous RNA to regulate ATAD2 expression by sponging microRNA-106b-5p in papillary thyroid cancer vol.9, pp.3, 2018, https://doi.org/10.1038/s41419-018-0418-z
  20. Long non-coding RNA implicated in the invasion and metastasis of head and neck cancer: possible function and mechanisms vol.17, pp.1, 2018, https://doi.org/10.1186/s12943-018-0763-7
  21. Long Non-coding RNA NEAT1: A Novel Target for Diagnosis and Therapy in Human Tumors vol.9, pp.1664-8021, 2018, https://doi.org/10.3389/fgene.2018.00471
  22. Galectin-3 activates TLR4/NF-κB signaling to promote lung adenocarcinoma cell proliferation through activating lncRNA-NEAT1 expression vol.18, pp.1, 2018, https://doi.org/10.1186/s12885-018-4461-z
  23. Up-regulation of CTD-2547G23.4 in hepatocellular carcinoma tissues and its prospective molecular regulatory mechanism: a novel qRT-PCR and bioinformatics analysis study vol.18, pp.1, 2018, https://doi.org/10.1186/s12935-018-0566-3