Journal of Nutrition and Health

  • 제48권4호
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  • Pages.299-309
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  • 2015
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  • 2288-3886(pISSN)
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  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
권호 표지
DOI QR코드

DOI QR Code

Streptozotocin 유도 당뇨쥐에서 C3G 및 D3G 안토시안이 풍부한 검정콩 CJ-3호 종피 추출물의 혈중지질 개선효과

Effects of the C3G/D3G anthocyanins-rich black soybean testa extracts on improvement of lipid profiles in STZ-induced diabetic rats

  • 박소영 (성신여자대학교 비만과학연구소) ;
  • 박수정 (성신여자대학교 비만과학연구소) ;
  • 강송주 (성신여자대학교 식품영양학과) ;
  • 김나영 (성신여자대학교 식품영양학과) ;
  • 김다샘 (성신여자대학교 식품영양학과) ;
  • 김민진 (성신여자대학교 식품영양학과) ;
  • 김선아 (성신여자대학교 식품영양학과) ;
  • 김지영 (성신여자대학교 식품영양학과) ;
  • 박소연 (성신여자대학교 식품영양학과) ;
  • 박소현 (성신여자대학교 식품영양학과) ;
  • 윤초롱 (성신여자대학교 식품영양학과) ;
  • 이보람 (성신여자대학교 식품영양학과) ;
  • 이효은 (성신여자대학교 식품영양학과) ;
  • 최소영 (성신여자대학교 식품영양학과) ;
  • 최희원 (성신여자대학교 식품영양학과) ;
  • 허진여 (성신여자대학교 식품영양학과) ;
  • 황아영 (성신여자대학교 식품영양학과) ;
  • 이명숙 (성신여자대학교 비만과학연구소)
  • Park, So Young (Research Institute of Obesity Science, Sungshin Women's University) ;
  • Pak, Sujeong (Research Institute of Obesity Science, Sungshin Women's University) ;
  • Kang, Song Joo (Department of Food and Nutrition, Sungshin Women's University) ;
  • Kim, Na Young (Department of Food and Nutrition, Sungshin Women's University) ;
  • Kim, Da Saem (Department of Food and Nutrition, Sungshin Women's University) ;
  • Kim, Min Jin (Department of Food and Nutrition, Sungshin Women's University) ;
  • Kim, Seon Ah (Department of Food and Nutrition, Sungshin Women's University) ;
  • Kim, Ji Young (Department of Food and Nutrition, Sungshin Women's University) ;
  • Park, So Yeon (Department of Food and Nutrition, Sungshin Women's University) ;
  • Park, So Hyun (Department of Food and Nutrition, Sungshin Women's University) ;
  • Youn, Cho Rong (Department of Food and Nutrition, Sungshin Women's University) ;
  • Lee, Bo Ram (Department of Food and Nutrition, Sungshin Women's University) ;
  • Lee, Hyo Eun (Department of Food and Nutrition, Sungshin Women's University) ;
  • Choi, So Young (Department of Food and Nutrition, Sungshin Women's University) ;
  • Choi, Hee Won (Department of Food and Nutrition, Sungshin Women's University) ;
  • Heo, Jin Yeo (Department of Food and Nutrition, Sungshin Women's University) ;
  • Hwang, A Yeong (Department of Food and Nutrition, Sungshin Women's University) ;
  • Lee, Myoung Sook (Research Institute of Obesity Science, Sungshin Women's University)
  • 투고 : 2015.08.17
  • 심사 : 2015.08.27
  • 발행 : 2015.08.31
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초록

기존 연구에서 주로 사용하는 기타 재래종 검정콩 종피추출물 (콩단백질 혹은 총 안토시안)의 효과를 연구하는 경우와 달리, 본 연구에서는 재래종 서리태보다 C3G와 D3G 안토시안이 2배 이상 풍부한 검정콩 CJ-3호 종피추출물을 이용하였다. STZ로 유도된 당뇨쥐에 4주간 검정콩 CJ-3호 종피추출물을 처치한 결과 비록 체중감소, 혈당강하, 인슐린저항성 완화 등의 효과는 미비하지만 근육량이 유의적으로 증가하였는데 이는 일반적으로 항비만 식이요법에 따라 나타나는 근육소모를 보완하는 중요한 효과이다. STZ유도 당뇨 쥐에서 억제된 글리코겐이용률이 검정콩 CJ-3호 종피추출물에 의하여 증가한 것은 포도당이 에너지원으로 사용되면서 지방조직의 지질이용률을 억제하여 혈중 TG를 개선시켰고 간에서 HMG-CoA reductase 활성을 조절함으로써 혈청 TC를 낮추는데 기여하였다. 4주간의 투여기간을 연장한다면 향후 당뇨에서 발생하는 인슐린저항성 및 당화적 산화성억제에 따른 지질의 과산화물의 생성을 억제할 수 있는 가능성도 기대할 수 있겠다. 또한 검정콩 CJ-3호는 병 저항성이 높고 종실특성이 우수한 녹자엽 검정콩으로 생산성이 높아서 기능성에 대한 반복 실험을 확인한다면 기능성식품시장의 산업화도 가능하다. 따라서 C3G와 D3G 안토시안이 풍부한 기능성 식품의 경우 당뇨, 비만 및 암 등 난치병질환에서 나타나는 혈중지질 개선을 위한 임상적 효과 또한 기대해 볼 수 있겠다.

Purpose: The purpose of this study was to examine the effect of black soybean (CJ-3) testa extracts on lipid profiles in streptozotocin (STZ)-induced diabetic rats. Methods: One control group and four STZ-induced diabetic groups with different doses of black soybean (CJ-3) testa extracts treatment [0 mg/kg (diabetic control, EX), 250 mg/kg (EX-250), 500 mg/kg (EX-500), 1,000 mg/kg (EX-1000)] were orally administered for 4 weeks. Results: All CJ-3 treatment groups had remarkably lower serum triglyceride (TG) levels than that of EX group (p < 0.05) whereas hepatic TG contents did not show any differences. Results from serum total cholesterol (TC) concentrations of EX-250 and EX-1000 groups were decreased compared to EX group (p < 0.05). Furthermore, protein levels of 3-hydroxy-3-methyl-glutaryl-Coenzyme A (HMG-CoA) reductase from the liver decreased in all treatment groups (p < 0.05). However, significant differences were not observed in serum glucose and insulin, and glucose transporter 4 (GLUT-4) protein expression in skeletal muscle tissue. Conclusion: These results suggest that black soybean testa extracts could be useful for improvement of hyperlipidemia and hypercholesteremia in diabetes.

키워드

참고문헌

  1. Statistics Korea. Diabetes : ${\geq}$30 years, by sex. Daejeon: Statistics Korea; 2013.
  2. Jung SH. Health and medicine. Seoul: Shinil books; 2007.
  3. Park KS. Insulin resistance and insulin resistance syndrome. Hanyang Med Rev 2009; 29(2): 130-133.
  4. Park HR, Cho JS. Effects of a natural medicinal multi-plant extract on blood glucose, insulin levels, and serum malondialdehyde concentrations in streptozotocin-induced diabetic rats. J East Asian Soc Diet Life 2007; 17(2): 205-212.
  5. Hong JH, Park MR, Rhee SJ. Effects of YK-209 mulberry leaves on HMG-CoA reductase and lipid composition of liver on streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 2002; 31(5): 826-833. https://doi.org/10.3746/jkfn.2002.31.5.826
  6. Urano S, Hoshi-Hashizume M, Tochigi N, Matsuo M, Shiraki M, Ito H. Vitamin E and the susceptibility of erythrocytes and reconstituted liposomes to oxidative stress in aged diabetics. Lipids 1991; 26(1): 58-61. https://doi.org/10.1007/BF02544025
  7. Bae EH, Moon GS. A study on the antioxidative activities of Korean soybeans. J Korean Soc Food Sci Nutr 1997; 26(2): 203-208.
  8. Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000; 63(7): 1035-1042. https://doi.org/10.1021/np9904509
  9. Cui CB, Choi HT, Lee HJ, Moon SY, Kim SH, Lee BG, Lee DS, Ham SS. Hypoglycemic effect of the functional food manufactured by fermented soybean as main materials in streptozotosininduced diabetic rats. J Korean Soc Food Sci Nutr 2004; 33(7): 1126-1132. https://doi.org/10.3746/jkfn.2004.33.7.1126
  10. Byun JS, Han YS, Lee SS. The effects of yellow soybean, black soybean, and sword bean on lipid levels and oxidative stress in ovariectomized rats. Int J Vitam Nutr Res 2010; 80(2): 97-106. https://doi.org/10.1024/0300-9831/a000010
  11. United States Department of Health & Human Services; United States Department of Agriculture. Scientific report of the 2015 dietary guidelines advisory committee [Internet]. Washington, D.C.: United States Department of Health & Human Services; United States Department of Agriculture; 2015 [cited 2015 Jan 28]. Available from: http://health.gov/dietaryguidelines/2015-scientific-report/PDFs/Scientific-Report-of-the-2015-Dietary-Guidelines-Advisory-Committee.pdf.
  12. Ranilla LG, Genovese MI, Lajolo FM. Polyphenols and antioxidant capacity of seed coat and cotyledon from Brazilian and Peruvian bean cultivars (Phaseolus vulgaris L.). J Agric Food Chem 2007; 55(1): 90-98. https://doi.org/10.1021/jf062785j
  13. Kim SY, Wi HR, Choi S, Ha TJ, Lee BW, Lee M. Inhibitory effect of anthocyanin-rich black soybean testa (Glycine max (L.) Merr.) on the inflammation-induced adipogenesis in a DIO mouse model. J Funct Foods 2015; 14: 623-633. https://doi.org/10.1016/j.jff.2015.02.030
  14. Kim YH, Kim DS, Woo SS, Kim HH, Lee YS, Kim HS, Ko KO, Lee SK. Antioxidant activity and cytotoxicity on human cancer cells of anthocyanin extracted from black soybean. Korean J Crop Sci 2008; 53(4): 407-412.
  15. Kim NY, Lee YD, Cho SC, Shin YC, Lee HY. Enhancement of anti-inflammation effect by fermentation process in Aronia melanocarpa (Michx.) Elliott extract. Korean J Med Crop Sci 2014;22(6): 475-482. https://doi.org/10.7783/KJMCS.2014.22.6.475
  16. Choung MG, Lim JD. Antioxidant, anticancer and immune activation of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Korean J Med Crop Sci 2012; 20(4): 259-269. https://doi.org/10.7783/KJMCS.2012.20.4.259
  17. Kim HB. Quantification of cyanidin-3-glucoside (C3G) in mulberry fruits and grapes. Korean J Seric Sci 2003; 45(1): 1-5.
  18. Lee JH, Kang NS, Shin SO, Shin SH, Lim SG, Suh DY, Baek IY, Park KY, Ha TJ. Characterisation of anthocyanins in the black soybean (Glycine max L.) by HPLC-DAD-ESI/MS analysis. Food Chem 2009; 112(1): 226-231. https://doi.org/10.1016/j.foodchem.2008.05.056
  19. Ha TJ, Lee MH, Park CH, Pae SB, Shim KB, Ko JM, Shin SO, Baek IY, Park KY. Identification and characterization of anthocyanins in yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) by high-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (HPLCDAD-ESI/MS) analysis. J Agric Food Chem 2010; 58(4): 2571-2576. https://doi.org/10.1021/jf903883e
  20. Nader GA, Esser KA. Intracellular signaling specificity in skeletal muscle in response to different modes of exercise. J Appl Physiol (1985) 2001; 90(5): 1936-1942. https://doi.org/10.1152/jappl.2001.90.5.1936
  21. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957; 226(1): 497-509.
  22. Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med 1990; 9(6): 515-540. https://doi.org/10.1016/0891-5849(90)90131-2
  23. Wasowicz W, Neve J, Peretz A. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem 1993; 39(12): 2522-2526.
  24. Lee HJ, Hwang EH. Effects of Artemisia capillaris Thunberg on the plasma and liver lipid metabolism in rats. J Nutr Health 2002; 35(4): 421-430.
  25. Nizamutdinova IT, Jin YC, Chung JI, Shin SC, Lee SJ, Seo HG, Lee JH, Chang KC, Kim HJ. The anti-diabetic effect of anthocyanins in streptozotocin-induced diabetic rats through glucose transporter 4 regulation and prevention of insulin resistance and pancreatic apoptosis. Mol Nutr Food Res 2009; 53(11): 1419-1429. https://doi.org/10.1002/mnfr.200800526
  26. Bucci D, Rodriguez-Gil JE, Vallorani C, Spinaci M, Galeati G, Tamanini C. GLUTs and mammalian sperm metabolism. J Androl 2011; 32(4): 348-355. https://doi.org/10.2164/jandrol.110.011197
  27. Morgan BJ, Chai SY, Albiston AL. GLUT4 associated proteins as therapeutic targets for diabetes. Recent Pat Endocr Metab Immune Drug Discov 2011; 5(1): 25-32. https://doi.org/10.2174/187221411794351914
  28. Shin JH, Lee SJ, Seo JK, Lee HJ, Ju JC, Sung NJ. Effect of a combined extract of Orostachys japonicus with medicinal plants on the lipid composition of the liver and kidney from streptozotocininduced diabetic rats. J Korean Soc Food Sci Nutr 2012; 41(4): 510-518. https://doi.org/10.3746/jkfn.2012.41.4.510
  29. Cho HK. Diabetes mellitus and disorder of lipid metabolism. J Korean Soc Endocrinol 2006; 21(2): 101-105. https://doi.org/10.3803/jkes.2006.21.2.101
  30. Liao Z, Chen X, Wu M. Antidiabetic effect of flavones from Cirsium japonicum DC in diabetic rats. Arch Pharm Res 2010; 33(3): 353-362. https://doi.org/10.1007/s12272-010-0302-6
  31. Kwon SH, Ahn IS, Kim SO, Kong CS, Chung HY, Do MS, Park KY. Anti-obesity and hypolipidemic effects of black soybean anthocyanins. J Med Food 2007; 10(3): 552-556. https://doi.org/10.1089/jmf.2006.147
  32. Ko MK, Kwon TW, Song YS. Effects of yellow and black soybeans on plasma and hepatic lipid composition and fecal lipid excretion in rats. J Korean Soc Food Sci Nutr 1998; 27(1): 126-131.
  33. Lee JW, Lee SM, Gwak KS, Lee JY, Choi IG. Screening of edible mushrooms for the production of lovastatin and its HMG-CoA Reductase inhibitory activity. Korean J Microbiol 2006; 42(2): 83-88.
  34. Jung JH, Kim HS. The inhibitory effect of black soybean on hepatic cholesterol accumulation in high cholesterol and high fat diet-induced non-alcoholic fatty liver disease. Food Chem Toxicol 2013; 60: 404-412. https://doi.org/10.1016/j.fct.2013.07.048
  35. Yu RQ, Wu XY, Zhou X, Zhu J, Ma LY. Cyanidin-3-glucoside attenuates body weight gain, serum lipid concentrations and insulin resistance in high-fat diet-induced obese rats. Zhongguo Dang Dai Er Ke Za Zhi 2014; 16(5): 534-538.
  36. Shin SH, Park DS, Jang MJ, Jeon JH, O NG, Song JY, Lee JS, Kim BY, Joo SS, Hwang SY, Kim YB. Protective effect of anthocyanins against the hepatotoxicity of carbon tetrachloride in rats. Lab Anim Res 2008; 24(3): 355-360.

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  2. The Positive Effect of LuoHanGuo as Sugar Substitute on Blood Glucose and Metabolism in Streptozotocin-Induced Diabetic Mice vol.46, pp.3, 2015, https://doi.org/10.9729/am.2016.46.3.140
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  4. Anthocyanin fingerprinting and dynamics in differentially pigmented exotic soybean genotypes using modified HPLC-DAD method vol.14, pp.4, 2015, https://doi.org/10.1007/s11694-020-00443-y
  5. Black Soybean and Adzuki Bean Extracts Lower Blood Pressure by Modulating the Renin-Angiotensin System in Spontaneously Hypertensive Rats vol.10, pp.7, 2015, https://doi.org/10.3390/foods10071571