Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of Diagnostic PCR System for Three Seedtransmitted Quarantine Viruses Associated with Cucurbitaceae

박과류 관련 종자전염 검역바이러스 3종의 PCR 진단시스템 개발

  • Lee, Siwon (Environmental Infrastructure Research Department, National Institute of Environmental Research) ;
  • Min, Byung-Dae (Environmental Infrastructure Research Department, National Institute of Environmental Research) ;
  • Lee, Jin-Young (Incheon International Airport Regional Office, Animal and Plant Quarantine Agency) ;
  • Shin, Yong-Gil (Incheon International Airport Regional Office, Animal and Plant Quarantine Agency) ;
  • Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University) ;
  • Jheong, Weon Hwa (Environmental Infrastructure Research Department, National Institute of Environmental Research)
  • 이시원 (국립환경과학원 환경기반연구부) ;
  • 민병대 (국립환경과학원 환경기반연구부) ;
  • 이진영 (농림축산검역본부 인천공항지역본부) ;
  • 신용길 (농림축산검역본부 인천공항지역본부) ;
  • 이수헌 (경북대학교 응용생명과학부) ;
  • 정원화 (국립환경과학원 환경기반연구부)
  • Received : 2014.10.31
  • Accepted : 2014.12.12
  • Published : 2015.03.28
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Abstract

The Cucurbitaceae are a plant family that consist of over a hundred genera, the most important of which are squash, pumpkin, zucchini, some gourds, cucumber and watermelon. These are among the top imported seeds in Korea. At the time of their import, the Squash mosaic virus (SqMV), the Cucumber green mottle mosaic virus (CGMMV) and the Kyuri green mottle mosaic virus (KGMMV) are designated as regulated viruses for quarantine in Korea. This study was conducted to develop specific primer sets for easy and rapid detection of SqMV, CGMMV and KGMMV. RT-PCR with the nested PCR primer sets and modified positive control plasmids were capable of highly sensitive detection and verification of such viruses. In addition, developed diagnostic PCR systems applied to quarantine sites detected 47 cases of SqMV, 67 cases of CGMMV and 17 cases of KGMMV between 2010 and the first half of 2014.

박과는 스쿼시, 호박, 애호박, 조롱박, 오이 및 수박 등 100여 속이 넘는 속이 포함된 식물과이며, 종자의 수입량이 매우 많은 작물이다. 이들이 우리나라로 수입될 때, 규제바이러스인 Squash mosaic virus (SqMV), Cucumber green mottle mosaic virus (CGMMV) 및 Kyuri green mottle mosaic virus (KGMMV)에 대하여 검역을 수행한다. 본 연구에서는 SqMV, CGMMV 및 KGMMV를 신속·높은 감도로 검출할 수 있는 특이적 RT-PCR 및 nested PCR 프라이머 조합과 유전자변형-양성대조구 플라스미드를 개발하였다. 또한 본 연구에서 개발한 진단시스템을 2010년부터 2014년 상반기까지 현장적용하여, SqMV 47건, CGMMV 67건 및 KGMMV 17건을 검출하였다.

Keywords

References

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